ADF/cofilin mediates actin cytoskeletal alterations in LLC-PK cells during ATP depletion

Sharon L. Ashworth, Erica L. Southgate, Ruben M. Sandoval, Peter J. Meberg, James R. Bamburg, Bruce A. Molitoris

Research output: Contribution to journalArticle

45 Scopus citations


Ischemic injury induces actin cytoskeleton disruption and aggregation, but mechanisms affecting these changes remain unclear. To determine the role of actin-depolymerizing factor (ADF)/ cofilin participation in ischemic-induced actin cytoskeletal breakdown, we utilized porcine kidney cultured cells, LLC-PKA4.8, and adenovirus containing wild-type (wt), constitutively active, and inactive Xenopus ADF/cofilin linked to green fluorescence protein [XAC(wt)-GFP] in an ATP depletion model. High adenoviral infectivity (70%) in LLC-PKA4.8 cells resulted in linearly increasing XAC(wt)-GFP and phosphorylated (p)XAC(wt)-GFP (inactive) expression. ATP depletion rapidly induced dephosphorylation, and, therefore, activation, of endogenous pcofilin as well as pXAC(wt)-GFP in conjunction with the formation of fluorescent XAC(wt)-GFP/actin aggregates and rods. No significant actin cytoskeletal alterations occurred with short-term ATP depletion of LLC-PKA4.8 cells expressing GFP or the constitutively inactive mutant XAC(S3E)-GFP, but cells expressing the constitutively active mutant demonstrated nearly instantaneous actin disruption with aggregate and rod formation. Confocal image three-dimensional volume reconstructions of normal and ATP-depleted LLC-PKA4.8 cells demonstrated that 25 min of ATP depletion induced a rapid increase in XAC(wt)-GFP apical and basal signal in addition to XAC-GFP/actin aggregate formation. These data demonstrate XAC(wt)-GFP participates in ischemia-induced actin cytoskeletal alterations and determines the rate and extent of these ATP depletion-induced cellular alterations.

Original languageEnglish (US)
Pages (from-to)F852-F862
JournalAmerican Journal of Physiology - Renal Physiology
Issue number4 53-4
StatePublished - Apr 1 2003


  • Actin-depolymerizing factor
  • Ischemia
  • Microvilli

ASJC Scopus subject areas

  • Physiology

Fingerprint Dive into the research topics of 'ADF/cofilin mediates actin cytoskeletal alterations in LLC-PK cells during ATP depletion'. Together they form a unique fingerprint.

  • Cite this