Adipokines and cytokines in human pancreatic juice

Unraveling the local pancreatic inflammatory milieu

Kathryn M. Dalbec, C. Schmidt, Terence E. Wade, Sue Wang, Deborah A. Swartz-Basile, Henry A. Pitt, Nicholas Zyromski

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Differential adipokine expression in obesity influences the inflammatory milieu, and may explain in part obesity's negative impact on pancreatic disease. Pancreatic juice analysis may provide a good means to evaluate the local pancreatic inflammatory milieu. The presence of adipokines in pancreatic juice is unknown. Aims: This proof-of-concept study was designed to determine the presence of adipokines and cytokines in human pancreatic juice. Methods: With institutional review board approval, pancreatic juice was obtained from ten patients with a broad range of diagnoses at the time of endoscopic retrograde cholangiopancreatography. Pancreatic juice was assayed using enzyme-linked immunosorbent assay (ELISA) for insulin, the proinflammatory adipokine leptin, the anti-inflammatory adipokine adiponectin, and the inflammatory mediators interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and monocyte chemoattractant protein-1 (MCP-1). Correlation between adipokine and inflammatory mediator expression was determined by linear regression analysis. Data are presented as mean ± standard error of the mean (SEM); P < 0.05 was considered statistically significant. Results: Leptin (0.16 ± 0.05 ng/ml) and adiponectin (0.63 ± 0.02 μg/ml) were both present, as were the inflammatory mediators IL-6 (112.6 ± 28.1 pg/ml), TNF-α (49.0 ± 18.8 pg/ml), and MCP-1 (32.2 ± 0.9 pg/ml). Paradoxically, the expression of the anti-inflammatory adipokine adiponectin correlated strongly with that of the proinflammatory cytokine IL-6 (R 2 = 0.98, P < 0.001). Conclusions: This report is the first to describe adipokine expression in human pancreatic juice. Human pancreatic juice inflammatory mediators and adipokines may provide an important measurement of the local pancreatic inflammatory milieu.

Original languageEnglish
Pages (from-to)2108-2112
Number of pages5
JournalDigestive Diseases and Sciences
Volume55
Issue number7
DOIs
StatePublished - Jul 2010

Fingerprint

Pancreatic Juice
Adipokines
Cytokines
Adiponectin
Interleukin-6
Chemokine CCL2
Leptin
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
Obesity
Pancreatic Diseases
Endoscopic Retrograde Cholangiopancreatography
Research Ethics Committees
Linear Models
Enzyme-Linked Immunosorbent Assay
Regression Analysis
Insulin

Keywords

  • Adipokines
  • Adiponectin
  • Inflammation
  • Leptin
  • Pancreatic juice

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Adipokines and cytokines in human pancreatic juice : Unraveling the local pancreatic inflammatory milieu. / Dalbec, Kathryn M.; Schmidt, C.; Wade, Terence E.; Wang, Sue; Swartz-Basile, Deborah A.; Pitt, Henry A.; Zyromski, Nicholas.

In: Digestive Diseases and Sciences, Vol. 55, No. 7, 07.2010, p. 2108-2112.

Research output: Contribution to journalArticle

Dalbec, Kathryn M. ; Schmidt, C. ; Wade, Terence E. ; Wang, Sue ; Swartz-Basile, Deborah A. ; Pitt, Henry A. ; Zyromski, Nicholas. / Adipokines and cytokines in human pancreatic juice : Unraveling the local pancreatic inflammatory milieu. In: Digestive Diseases and Sciences. 2010 ; Vol. 55, No. 7. pp. 2108-2112.
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T2 - Unraveling the local pancreatic inflammatory milieu

AU - Dalbec, Kathryn M.

AU - Schmidt, C.

AU - Wade, Terence E.

AU - Wang, Sue

AU - Swartz-Basile, Deborah A.

AU - Pitt, Henry A.

AU - Zyromski, Nicholas

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AB - Background: Differential adipokine expression in obesity influences the inflammatory milieu, and may explain in part obesity's negative impact on pancreatic disease. Pancreatic juice analysis may provide a good means to evaluate the local pancreatic inflammatory milieu. The presence of adipokines in pancreatic juice is unknown. Aims: This proof-of-concept study was designed to determine the presence of adipokines and cytokines in human pancreatic juice. Methods: With institutional review board approval, pancreatic juice was obtained from ten patients with a broad range of diagnoses at the time of endoscopic retrograde cholangiopancreatography. Pancreatic juice was assayed using enzyme-linked immunosorbent assay (ELISA) for insulin, the proinflammatory adipokine leptin, the anti-inflammatory adipokine adiponectin, and the inflammatory mediators interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and monocyte chemoattractant protein-1 (MCP-1). Correlation between adipokine and inflammatory mediator expression was determined by linear regression analysis. Data are presented as mean ± standard error of the mean (SEM); P < 0.05 was considered statistically significant. Results: Leptin (0.16 ± 0.05 ng/ml) and adiponectin (0.63 ± 0.02 μg/ml) were both present, as were the inflammatory mediators IL-6 (112.6 ± 28.1 pg/ml), TNF-α (49.0 ± 18.8 pg/ml), and MCP-1 (32.2 ± 0.9 pg/ml). Paradoxically, the expression of the anti-inflammatory adipokine adiponectin correlated strongly with that of the proinflammatory cytokine IL-6 (R 2 = 0.98, P < 0.001). Conclusions: This report is the first to describe adipokine expression in human pancreatic juice. Human pancreatic juice inflammatory mediators and adipokines may provide an important measurement of the local pancreatic inflammatory milieu.

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