Adiponectin Receptor-1 Expression Is Decreased in the Pancreas of Obese Mice

Terence E. Wade, Abhishek Mathur, Debao Lu, Deborah A. Swartz-Basile, Henry A. Pitt, Nicholas Zyromski

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: Obesity is epidemic in the 21st century and has been shown to be a risk factor for developing severe acute pancreatitis. Adipose tissue produces small molecules called adipokines, which are important in modulating metabolism and inflammation. The anti-inflammatory adipokine adiponectin is decreased in obesity and inversely mirrors the severity of pancreatitis in a murine experimental model. Adiponectin acts through two receptors, AdipoR1 and AdipoR2; no data are currently available regarding adiponection receptor expression in the obese murine pancreas. Materials and methods: Immunohistochemical and reverse transcription-polymerase chain reaction analysis were undertaken to determine expression of adiponectin receptors AdipoR1 and AdipoR2 in the pancreas and liver of lean (C57BL/6J) and congenitally obese (LepOb and LepDb) mice. Results: Immunohistochemistry confirmed expression of both AdipoR1 and AdipoR2 in the pancreas of all three murine strains. Staining was positive in acinar cells and to a lesser extent in islet cells. Pancreatic gene expression of AdipoR2 was similar among lean and obese mice. AdipoR1 gene expression, however, was significantly (P < 0.001) decreased in the pancreas of both LepOb and LepDb mice compared to wild-type lean animals. Gene expression of both AdipoR1 and AdipoR2 was significantly less in the liver of obese (LepOb and LepDb) mice compared to wild-type lean animals (P < 0.001). Conclusions: These data show for the first time that the adiponectin receptors AdipoR1 and AdipoR2 are expressed in the obese murine pancreas. The paucity of AdipoR1 receptors may be important when considering the role played by adipokines in the genesis of severe pancreatitis in obesity.

Original languageEnglish
Pages (from-to)78-84
Number of pages7
JournalJournal of Surgical Research
Volume154
Issue number1
DOIs
StatePublished - Jun 1 2009

Fingerprint

Adiponectin Receptors
Obese Mice
Pancreas
Adipokines
Pancreatitis
Wild Animals
Obesity
Adiponectin
Gene Expression
Acinar Cells
Liver
Islets of Langerhans
Reverse Transcription
Adipose Tissue
Anti-Inflammatory Agents
Theoretical Models
Immunohistochemistry
Staining and Labeling
Inflammation
Polymerase Chain Reaction

Keywords

  • adiponectin
  • adiponectin receptors
  • obesity
  • pancreatitis

ASJC Scopus subject areas

  • Surgery

Cite this

Adiponectin Receptor-1 Expression Is Decreased in the Pancreas of Obese Mice. / Wade, Terence E.; Mathur, Abhishek; Lu, Debao; Swartz-Basile, Deborah A.; Pitt, Henry A.; Zyromski, Nicholas.

In: Journal of Surgical Research, Vol. 154, No. 1, 01.06.2009, p. 78-84.

Research output: Contribution to journalArticle

Wade, Terence E. ; Mathur, Abhishek ; Lu, Debao ; Swartz-Basile, Deborah A. ; Pitt, Henry A. ; Zyromski, Nicholas. / Adiponectin Receptor-1 Expression Is Decreased in the Pancreas of Obese Mice. In: Journal of Surgical Research. 2009 ; Vol. 154, No. 1. pp. 78-84.
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AU - Pitt, Henry A.

AU - Zyromski, Nicholas

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AB - Background: Obesity is epidemic in the 21st century and has been shown to be a risk factor for developing severe acute pancreatitis. Adipose tissue produces small molecules called adipokines, which are important in modulating metabolism and inflammation. The anti-inflammatory adipokine adiponectin is decreased in obesity and inversely mirrors the severity of pancreatitis in a murine experimental model. Adiponectin acts through two receptors, AdipoR1 and AdipoR2; no data are currently available regarding adiponection receptor expression in the obese murine pancreas. Materials and methods: Immunohistochemical and reverse transcription-polymerase chain reaction analysis were undertaken to determine expression of adiponectin receptors AdipoR1 and AdipoR2 in the pancreas and liver of lean (C57BL/6J) and congenitally obese (LepOb and LepDb) mice. Results: Immunohistochemistry confirmed expression of both AdipoR1 and AdipoR2 in the pancreas of all three murine strains. Staining was positive in acinar cells and to a lesser extent in islet cells. Pancreatic gene expression of AdipoR2 was similar among lean and obese mice. AdipoR1 gene expression, however, was significantly (P < 0.001) decreased in the pancreas of both LepOb and LepDb mice compared to wild-type lean animals. Gene expression of both AdipoR1 and AdipoR2 was significantly less in the liver of obese (LepOb and LepDb) mice compared to wild-type lean animals (P < 0.001). Conclusions: These data show for the first time that the adiponectin receptors AdipoR1 and AdipoR2 are expressed in the obese murine pancreas. The paucity of AdipoR1 receptors may be important when considering the role played by adipokines in the genesis of severe pancreatitis in obesity.

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