Adipose stem cell function maintained with age: An intra-subject study of long-term cryopreserved cells

Lauren E. Kokai, Dmitry Traktuev, Liyong Zhang, Stephanie Merfeld-Clauss, Gabriella Dibernardo, Hongyan Lu, Kacey G. Marra, Albert Donnenberg, Vera Donnenberg, E. Michael Meyer, Peter B. Fodor, Keith L. March, J. Peter Rubin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: The progressive decline in tissue mechanical strength that occurs with aging is hypothesized to be due to a loss of resident stem cell number and function. As such, there is concern regarding use of autologous adult stem cell therapy in older patients. To abrogate this, many patients elect to cryopreserve the adipose stromal-vascular fraction (SVF) of lipoaspirate, which contains resident adipose stem cells (ASC). However, it is not clear yet if there is any clinical benefit from banking cells at a younger age. Objectives: We performed a comparative analysis of SVF composition and ASC function from cells obtained under GMP conditions from the same three patients with time gap of 7 to 12 years. Methods: SVF, cryobanked under good manufacturing practice (GMP) conditions, was thawed and cell yield, viability, and cellular composition were assessed. In parallel, ASC proliferation and efficiency of tri-lineage differentiation were evaluated. Results: The results showed no significant differences existed in cell yield and SVF subpopulation composition within the same patient between harvest procedures 7 to 12 years apart. Further, no change in proliferation rates of cultured ASCs was found, and expanded cells from all patients were capable of tri-lineage differentiation. Conclusions: By harvesting fat from the same patient at two time points, we have shown that despite the natural human aging process, the prevalence and functional activity of ASCs in an adult mesenchymal stem cell, is highly preserved.

Original languageEnglish (US)
Pages (from-to)454-463
Number of pages10
JournalAesthetic Surgery Journal
Volume37
Issue number4
DOIs
StatePublished - Apr 1 2017

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Stem Cells
Blood Vessels
Adult Stem Cells
Stromal Cells
Cell- and Tissue-Based Therapy
Mesenchymal Stromal Cells
Cell Survival
Cell Count
Fats
Cell Proliferation

ASJC Scopus subject areas

  • Surgery
  • Medicine(all)

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Adipose stem cell function maintained with age : An intra-subject study of long-term cryopreserved cells. / Kokai, Lauren E.; Traktuev, Dmitry; Zhang, Liyong; Merfeld-Clauss, Stephanie; Dibernardo, Gabriella; Lu, Hongyan; Marra, Kacey G.; Donnenberg, Albert; Donnenberg, Vera; Meyer, E. Michael; Fodor, Peter B.; March, Keith L.; Rubin, J. Peter.

In: Aesthetic Surgery Journal, Vol. 37, No. 4, 01.04.2017, p. 454-463.

Research output: Contribution to journalArticle

Kokai, LE, Traktuev, D, Zhang, L, Merfeld-Clauss, S, Dibernardo, G, Lu, H, Marra, KG, Donnenberg, A, Donnenberg, V, Meyer, EM, Fodor, PB, March, KL & Rubin, JP 2017, 'Adipose stem cell function maintained with age: An intra-subject study of long-term cryopreserved cells', Aesthetic Surgery Journal, vol. 37, no. 4, pp. 454-463. https://doi.org/10.1093/asj/sjw197
Kokai, Lauren E. ; Traktuev, Dmitry ; Zhang, Liyong ; Merfeld-Clauss, Stephanie ; Dibernardo, Gabriella ; Lu, Hongyan ; Marra, Kacey G. ; Donnenberg, Albert ; Donnenberg, Vera ; Meyer, E. Michael ; Fodor, Peter B. ; March, Keith L. ; Rubin, J. Peter. / Adipose stem cell function maintained with age : An intra-subject study of long-term cryopreserved cells. In: Aesthetic Surgery Journal. 2017 ; Vol. 37, No. 4. pp. 454-463.
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