Adipose stem cells-conditioned medium blocks 6-hydroxydopamine-induced neurotoxicity via the IGF-1/PI3K/AKT pathway

Xianjun Wang, Zhenyu Zhao, Jian Gong, Shengnian Zhou, Hongjun Peng, Adam Shatara, Timmy Z. Zhu, Rebecca Meltzer, Yansheng Du, Huiying Gu

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Previous studies suggest that the delivery of neurotrophic factors secreted from adipose stromal cells (ASC) protect the brain from 6-hydroxydopamine (6-OHDA)-induced neurotoxicity. However, it remains unclear which secreted neurotrophic factor has an important role in protecting 6-OHDA-treated neurons. Through the use of antibodies in this study, we demonstrated that specific neutralization of IGF-1 activity in ASC conditioned media (ASC-CM) significantly blocks ASC-CM-induced neuroprotection against 6-OHDA neurotoxicity. Consistently, this neuroprotection was mostly attributed to the activation of the AKT-mediated signaling pathway. In contrast, brain derived neurotrophic factor (BDNF), glial-derived neurotrophic factor (GDNF), vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) in ASC-CM did not play a role in ASC-CM-induced neuroprotection against 6-OHDA.

Original languageEnglish (US)
Pages (from-to)98-102
Number of pages5
JournalNeuroscience Letters
Volume581
DOIs
StatePublished - Oct 3 2014

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Keywords

  • 6-hydroxydopamine
  • AKT
  • ASC-CM
  • IGF-1
  • Neuronal cell death

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

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