Adipose stromal cells-conditional medium protected glutamate-induced CGNs neuronal death by BDNF

L. Zhao, X. Wei, Z. Ma, D. Feng, P. Tu, B. H. Johnstone, K. L. March, Y. Du

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

The delivery of factors secreted by adipose stromal cells (ASC) to the brain may be a viable neuroprotective therapeutic option. In this study, we investigated the bioactivity of ASC-conditioned medium (ASC-CM) in glutamate-induced neurotoxicity and found that the ASC-CM significantly blocked glutamate neurotoxicity. We identified the brain-derived neurotrophic factor (BDNF) in the ASC-CM by using Western blot and demonstrated that this activity was critical for the neuroprotective effect of ASC-CM in excytotoxicity models. Furthermore, inactivating BDNF also attenuated the suppression by ASC-CM of glutamate-induced caspase-3 activity, but not p38 phosphorylation. These findings suggest that among ASC secrete a potent combination of factors, BDNF play a major role in neuroprotection against excytotoxicity.

Original languageEnglish (US)
Pages (from-to)238-240
Number of pages3
JournalNeuroscience Letters
Volume452
Issue number3
DOIs
StatePublished - Mar 20 2009

Keywords

  • Adipose stromal cells
  • Brain-derived neurotrophic factor
  • Excytotoxicity
  • Neuroprotection

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Adipose stromal cells-conditional medium protected glutamate-induced CGNs neuronal death by BDNF'. Together they form a unique fingerprint.

  • Cite this