Adipose stromal cells-conditional medium protected glutamate-induced CGNs neuronal death by BDNF

L. Zhao, X. Wei, Z. Ma, D. Feng, P. Tu, B. H. Johnstone, K. L. March, Yansheng Du

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The delivery of factors secreted by adipose stromal cells (ASC) to the brain may be a viable neuroprotective therapeutic option. In this study, we investigated the bioactivity of ASC-conditioned medium (ASC-CM) in glutamate-induced neurotoxicity and found that the ASC-CM significantly blocked glutamate neurotoxicity. We identified the brain-derived neurotrophic factor (BDNF) in the ASC-CM by using Western blot and demonstrated that this activity was critical for the neuroprotective effect of ASC-CM in excytotoxicity models. Furthermore, inactivating BDNF also attenuated the suppression by ASC-CM of glutamate-induced caspase-3 activity, but not p38 phosphorylation. These findings suggest that among ASC secrete a potent combination of factors, BDNF play a major role in neuroprotection against excytotoxicity.

Original languageEnglish
Pages (from-to)238-240
Number of pages3
JournalNeuroscience Letters
Volume452
Issue number3
DOIs
StatePublished - Mar 20 2009

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Brain-Derived Neurotrophic Factor
Stromal Cells
Glutamic Acid
Neuroprotective Agents
Conditioned Culture Medium
Caspase 3
Western Blotting
Phosphorylation
Brain

Keywords

  • Adipose stromal cells
  • Brain-derived neurotrophic factor
  • Excytotoxicity
  • Neuroprotection

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Adipose stromal cells-conditional medium protected glutamate-induced CGNs neuronal death by BDNF. / Zhao, L.; Wei, X.; Ma, Z.; Feng, D.; Tu, P.; Johnstone, B. H.; March, K. L.; Du, Yansheng.

In: Neuroscience Letters, Vol. 452, No. 3, 20.03.2009, p. 238-240.

Research output: Contribution to journalArticle

Zhao, L. ; Wei, X. ; Ma, Z. ; Feng, D. ; Tu, P. ; Johnstone, B. H. ; March, K. L. ; Du, Yansheng. / Adipose stromal cells-conditional medium protected glutamate-induced CGNs neuronal death by BDNF. In: Neuroscience Letters. 2009 ; Vol. 452, No. 3. pp. 238-240.
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