Adipose stromal cells-conditioned medium blocks 6-hydroxydopamine-induced neurotoxicity and reactive oxygen species

Huiying Gu, Jimmy Wang, Nicole Du, Jiangning Tan, Brian Johnstone, Yansheng Du

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A recent in vivo study suggested that the delivery of adipose stromal cells (ASCs) protected rat brains from 6-hydroxydopamine (6-OHDA)-induced neurotoxicity. However, the molecular mechanism that underlies this neuroprotection remains unknown. It was suggested that ASCs-induced neuroprotection possibly resulting from released factors from ASCs. In this study, we investigated whether and how cell-free conditioned media collected from ASCs (ASC-CM) protect neurons against neurotoxicity induced by 6-OHDA in cultured rat rostral mesencephalic neurons (RMN) and cerebellar granule neurons (CGN). We now report that ASC-CM protects both RMN and CGN against 6-OHDA neurotoxicity. Exposure of CGN to 6-OHDA resulted in a significant increases in neuronal ROS and cell death. As expected, pretreatments with ASC-CM dramatically block both 6-OHDA-induced ROS and neurotoxicity. Additionally, ASC-CM also directly attenuated H2O2-induced neuronal death. Our results suggest that ASC-CM could block 6-OHDA-induced neuronal death by inhibiting both 6-OHDA-induced ROS generation and ROS-induced neurotoxicity in neurons. Both antioxidative and neuroprotective effects of ASC-CM may be beneficial in the therapy for Parkinson's disease and other neurodegenerative diseases.

Original languageEnglish
Pages (from-to)15-19
Number of pages5
JournalNeuroscience Letters
Volume544
DOIs
StatePublished - Jun 7 2013

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Oxidopamine
Stromal Cells
Conditioned Culture Medium
Reactive Oxygen Species
Neurons
Neuroprotective Agents
Neurodegenerative Diseases
Parkinson Disease
Cell Death

Keywords

  • 6-OHDA
  • Antioxidant
  • Neuronal cell death
  • Neurons
  • ROS

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Adipose stromal cells-conditioned medium blocks 6-hydroxydopamine-induced neurotoxicity and reactive oxygen species. / Gu, Huiying; Wang, Jimmy; Du, Nicole; Tan, Jiangning; Johnstone, Brian; Du, Yansheng.

In: Neuroscience Letters, Vol. 544, 07.06.2013, p. 15-19.

Research output: Contribution to journalArticle

Gu, Huiying ; Wang, Jimmy ; Du, Nicole ; Tan, Jiangning ; Johnstone, Brian ; Du, Yansheng. / Adipose stromal cells-conditioned medium blocks 6-hydroxydopamine-induced neurotoxicity and reactive oxygen species. In: Neuroscience Letters. 2013 ; Vol. 544. pp. 15-19.
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