Adipose stromal cells-secreted neuroprotective media against neuronal apoptosis

X. Wei, L. Zhao, J. Zhong, H. Gu, D. Feng, B. H. Johnstone, K. L. March, Martin Farlow, Yansheng Du

Research output: Contribution to journalArticle

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Abstract

Transplantation of pluripotent adipose stem/stromal cells (ASC) alleviates tissue damage and improves functional deficits in both stroke and cardiovascular disease animal models. Recent studies indicate that the primary mechanism of ASC-induced repair may not be directly related to tissue regeneration through differentiation, but rather through paracrine mechanisms provided by secreted pro-survival and repair-inducing trophic factors. In this study, we have found that ASC-conditioned medium (ASC-CM) potently protected cerebellar granule neurons (CGN) from apoptosis induced by serum and potassium deprivation. Neural cell protection was mostly attributable to activated caspase-3 and Akt-mediated neuroprotective pathway signaling. Specific neutralization of neurotrophic factor activity demonstrated that serum and potassium deprivation-induced Akt-mediated neuroprotection and caspase-3-dependent apoptosis were mainly modulated by IGF-1. These data suggest that of the many neuroprotective factors secreted by ASC, IGF-1 is the major factor that mediates protection against serum and potassium deprivation-induced CGN apoptosis. This study establishes a mechanistic basis supporting the therapeutic application of ASC for neurological disorders, specifically through paracrine support provided by trophic factor secretion.

Original languageEnglish
Pages (from-to)76-79
Number of pages4
JournalNeuroscience Letters
Volume462
Issue number1
DOIs
StatePublished - Sep 18 2009

Fingerprint

Stromal Cells
Stem Cells
Apoptosis
Potassium
Insulin-Like Growth Factor I
Caspase 3
Serum
Neurons
Cytoprotection
Nerve Growth Factors
Conditioned Culture Medium
Nervous System Diseases
Regeneration
Cardiovascular Diseases
Animal Models
Transplantation
Stroke
Neuroprotection
Therapeutics

Keywords

  • Adipose stromal cells
  • Apoptosis
  • Caspase-3
  • Cerebella granule neuron
  • Neuroprotection

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Adipose stromal cells-secreted neuroprotective media against neuronal apoptosis. / Wei, X.; Zhao, L.; Zhong, J.; Gu, H.; Feng, D.; Johnstone, B. H.; March, K. L.; Farlow, Martin; Du, Yansheng.

In: Neuroscience Letters, Vol. 462, No. 1, 18.09.2009, p. 76-79.

Research output: Contribution to journalArticle

Wei, X, Zhao, L, Zhong, J, Gu, H, Feng, D, Johnstone, BH, March, KL, Farlow, M & Du, Y 2009, 'Adipose stromal cells-secreted neuroprotective media against neuronal apoptosis', Neuroscience Letters, vol. 462, no. 1, pp. 76-79. https://doi.org/10.1016/j.neulet.2009.06.054
Wei, X. ; Zhao, L. ; Zhong, J. ; Gu, H. ; Feng, D. ; Johnstone, B. H. ; March, K. L. ; Farlow, Martin ; Du, Yansheng. / Adipose stromal cells-secreted neuroprotective media against neuronal apoptosis. In: Neuroscience Letters. 2009 ; Vol. 462, No. 1. pp. 76-79.
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