Adipose tissue macrophages function as antigen-presenting cells and regulate adipose tissue cd4+ t cells in mice

David L. Morris, Kae Won Cho, Jennifer L. DelProposto, Kelsie E. Oatmen, Lynn M. Geletka, Gabriel Martinez-Santibanez, Kanakadurga Singer, Carey N. Lumeng

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

The proinflammatory activation of leukocytes in adipose tissue contributes to metabolic disease. How crosstalk between immune cells initiates and sustains adipose tissue inflammation remains an unresolved question. We have examined the hypothesis that adipose tissue macrophages (ATMs) interact with and regulate the function of T cells. Dietary obesity was shown to activate the proliferation of effector memory CD4+ T cells in adipose tissue. Our studies further demonstrate that ATMs are functional antigen-presenting cells that promote the proliferation of interferon-g-producing CD4+ T cells in adipose tissue. ATMs from lean and obese visceral fat process and present major histocompatibility complex (MHC) class II-restricted antigens. ATMs were sufficient to promote proliferation and interferon-β production from antigen-specific CD4+ T cells in vitro and in vivo. Diet-induced obesity increased the expression of MHC II and T-cell costimulatory molecules on ATMs in visceral fat, which correlated with an induction of T-cell proliferation in that depot. Collectively, these data indicate that ATMs provide a functional link between the innate and adaptive immune systems within visceral fat in mice.

Original languageEnglish (US)
Pages (from-to)2762-2772
Number of pages11
JournalDiabetes
Volume62
Issue number8
DOIs
StatePublished - Aug 2013
Externally publishedYes

Fingerprint

Antigen-Presenting Cells
Adipose Tissue
Macrophages
T-Lymphocytes
Intra-Abdominal Fat
Major Histocompatibility Complex
Interferons
Obesity
Cell Proliferation
CD4 Antigens
Histocompatibility Antigens Class II
Metabolic Diseases
Immune System
Leukocytes
Diet
Inflammation

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Morris, D. L., Cho, K. W., DelProposto, J. L., Oatmen, K. E., Geletka, L. M., Martinez-Santibanez, G., ... Lumeng, C. N. (2013). Adipose tissue macrophages function as antigen-presenting cells and regulate adipose tissue cd4+ t cells in mice. Diabetes, 62(8), 2762-2772. https://doi.org/10.2337/db12-1404/-/DC1

Adipose tissue macrophages function as antigen-presenting cells and regulate adipose tissue cd4+ t cells in mice. / Morris, David L.; Cho, Kae Won; DelProposto, Jennifer L.; Oatmen, Kelsie E.; Geletka, Lynn M.; Martinez-Santibanez, Gabriel; Singer, Kanakadurga; Lumeng, Carey N.

In: Diabetes, Vol. 62, No. 8, 08.2013, p. 2762-2772.

Research output: Contribution to journalArticle

Morris, DL, Cho, KW, DelProposto, JL, Oatmen, KE, Geletka, LM, Martinez-Santibanez, G, Singer, K & Lumeng, CN 2013, 'Adipose tissue macrophages function as antigen-presenting cells and regulate adipose tissue cd4+ t cells in mice', Diabetes, vol. 62, no. 8, pp. 2762-2772. https://doi.org/10.2337/db12-1404/-/DC1
Morris DL, Cho KW, DelProposto JL, Oatmen KE, Geletka LM, Martinez-Santibanez G et al. Adipose tissue macrophages function as antigen-presenting cells and regulate adipose tissue cd4+ t cells in mice. Diabetes. 2013 Aug;62(8):2762-2772. https://doi.org/10.2337/db12-1404/-/DC1
Morris, David L. ; Cho, Kae Won ; DelProposto, Jennifer L. ; Oatmen, Kelsie E. ; Geletka, Lynn M. ; Martinez-Santibanez, Gabriel ; Singer, Kanakadurga ; Lumeng, Carey N. / Adipose tissue macrophages function as antigen-presenting cells and regulate adipose tissue cd4+ t cells in mice. In: Diabetes. 2013 ; Vol. 62, No. 8. pp. 2762-2772.
@article{940ddbc4303347d8b5558621b194d156,
title = "Adipose tissue macrophages function as antigen-presenting cells and regulate adipose tissue cd4+ t cells in mice",
abstract = "The proinflammatory activation of leukocytes in adipose tissue contributes to metabolic disease. How crosstalk between immune cells initiates and sustains adipose tissue inflammation remains an unresolved question. We have examined the hypothesis that adipose tissue macrophages (ATMs) interact with and regulate the function of T cells. Dietary obesity was shown to activate the proliferation of effector memory CD4+ T cells in adipose tissue. Our studies further demonstrate that ATMs are functional antigen-presenting cells that promote the proliferation of interferon-g-producing CD4+ T cells in adipose tissue. ATMs from lean and obese visceral fat process and present major histocompatibility complex (MHC) class II-restricted antigens. ATMs were sufficient to promote proliferation and interferon-β production from antigen-specific CD4+ T cells in vitro and in vivo. Diet-induced obesity increased the expression of MHC II and T-cell costimulatory molecules on ATMs in visceral fat, which correlated with an induction of T-cell proliferation in that depot. Collectively, these data indicate that ATMs provide a functional link between the innate and adaptive immune systems within visceral fat in mice.",
author = "Morris, {David L.} and Cho, {Kae Won} and DelProposto, {Jennifer L.} and Oatmen, {Kelsie E.} and Geletka, {Lynn M.} and Gabriel Martinez-Santibanez and Kanakadurga Singer and Lumeng, {Carey N.}",
year = "2013",
month = "8",
doi = "10.2337/db12-1404/-/DC1",
language = "English (US)",
volume = "62",
pages = "2762--2772",
journal = "Diabetes",
issn = "0012-1797",
publisher = "American Diabetes Association Inc.",
number = "8",

}

TY - JOUR

T1 - Adipose tissue macrophages function as antigen-presenting cells and regulate adipose tissue cd4+ t cells in mice

AU - Morris, David L.

AU - Cho, Kae Won

AU - DelProposto, Jennifer L.

AU - Oatmen, Kelsie E.

AU - Geletka, Lynn M.

AU - Martinez-Santibanez, Gabriel

AU - Singer, Kanakadurga

AU - Lumeng, Carey N.

PY - 2013/8

Y1 - 2013/8

N2 - The proinflammatory activation of leukocytes in adipose tissue contributes to metabolic disease. How crosstalk between immune cells initiates and sustains adipose tissue inflammation remains an unresolved question. We have examined the hypothesis that adipose tissue macrophages (ATMs) interact with and regulate the function of T cells. Dietary obesity was shown to activate the proliferation of effector memory CD4+ T cells in adipose tissue. Our studies further demonstrate that ATMs are functional antigen-presenting cells that promote the proliferation of interferon-g-producing CD4+ T cells in adipose tissue. ATMs from lean and obese visceral fat process and present major histocompatibility complex (MHC) class II-restricted antigens. ATMs were sufficient to promote proliferation and interferon-β production from antigen-specific CD4+ T cells in vitro and in vivo. Diet-induced obesity increased the expression of MHC II and T-cell costimulatory molecules on ATMs in visceral fat, which correlated with an induction of T-cell proliferation in that depot. Collectively, these data indicate that ATMs provide a functional link between the innate and adaptive immune systems within visceral fat in mice.

AB - The proinflammatory activation of leukocytes in adipose tissue contributes to metabolic disease. How crosstalk between immune cells initiates and sustains adipose tissue inflammation remains an unresolved question. We have examined the hypothesis that adipose tissue macrophages (ATMs) interact with and regulate the function of T cells. Dietary obesity was shown to activate the proliferation of effector memory CD4+ T cells in adipose tissue. Our studies further demonstrate that ATMs are functional antigen-presenting cells that promote the proliferation of interferon-g-producing CD4+ T cells in adipose tissue. ATMs from lean and obese visceral fat process and present major histocompatibility complex (MHC) class II-restricted antigens. ATMs were sufficient to promote proliferation and interferon-β production from antigen-specific CD4+ T cells in vitro and in vivo. Diet-induced obesity increased the expression of MHC II and T-cell costimulatory molecules on ATMs in visceral fat, which correlated with an induction of T-cell proliferation in that depot. Collectively, these data indicate that ATMs provide a functional link between the innate and adaptive immune systems within visceral fat in mice.

UR - http://www.scopus.com/inward/record.url?scp=84878800136&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878800136&partnerID=8YFLogxK

U2 - 10.2337/db12-1404/-/DC1

DO - 10.2337/db12-1404/-/DC1

M3 - Article

VL - 62

SP - 2762

EP - 2772

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 8

ER -