Administration of Ovalbumin Encapsulated in Alginate Microspheres to Mice

T. L. Bowersock, Harm HogenEsch, M. Suckow, John Turek, E. Davis-Snyder, D. Borie, R. Jackson, Haesun Park, Kinam Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Sodium alginate microparticles were tested as a delivery system for oral vaccines. Ovalbumin was incorporated into alginate microparticles. The release of ovalbumin from the microparticles was determined. Electron microscopy was performed to evaluate surface characteristics of the microparticles. Plain microspheres or ovalbumin incorporated within alginate microspheres were administered orally to groups of mice; another 2 groups were given either ovalbumin in microspheres or in Freund's adjuvant by subcutaneous injection. Antibodies specific to ovalbumin were measured in serum and antibody-secreting cells specific for ovalbumin were enumerated in spleen cells. Microparticles released ovalbumin within 5 days in vitro. Alginate microparticles were uniform in density and spherical in shape by electron microscopy. Mice had increased numbers of antibody secreting cells and serum antibody titers specific for ovalbumin regardless of the method of inoculation. These studies suggest that alginate microparticles could be used for the delivery of antigens. Further studies of this delivery system are warranted.

Original languageEnglish (US)
Pages (from-to)58-66
Number of pages9
JournalACS Symposium Series
Volume627
StatePublished - 1996
Externally publishedYes

Fingerprint

Alginate
Ovalbumin
Microspheres
Antibodies
Electron microscopy
Sodium alginate
Vaccines
Antigens
Freund's Adjuvant
alginic acid

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Bowersock, T. L., HogenEsch, H., Suckow, M., Turek, J., Davis-Snyder, E., Borie, D., ... Park, K. (1996). Administration of Ovalbumin Encapsulated in Alginate Microspheres to Mice. ACS Symposium Series, 627, 58-66.

Administration of Ovalbumin Encapsulated in Alginate Microspheres to Mice. / Bowersock, T. L.; HogenEsch, Harm; Suckow, M.; Turek, John; Davis-Snyder, E.; Borie, D.; Jackson, R.; Park, Haesun; Park, Kinam.

In: ACS Symposium Series, Vol. 627, 1996, p. 58-66.

Research output: Contribution to journalArticle

Bowersock, TL, HogenEsch, H, Suckow, M, Turek, J, Davis-Snyder, E, Borie, D, Jackson, R, Park, H & Park, K 1996, 'Administration of Ovalbumin Encapsulated in Alginate Microspheres to Mice', ACS Symposium Series, vol. 627, pp. 58-66.
Bowersock TL, HogenEsch H, Suckow M, Turek J, Davis-Snyder E, Borie D et al. Administration of Ovalbumin Encapsulated in Alginate Microspheres to Mice. ACS Symposium Series. 1996;627:58-66.
Bowersock, T. L. ; HogenEsch, Harm ; Suckow, M. ; Turek, John ; Davis-Snyder, E. ; Borie, D. ; Jackson, R. ; Park, Haesun ; Park, Kinam. / Administration of Ovalbumin Encapsulated in Alginate Microspheres to Mice. In: ACS Symposium Series. 1996 ; Vol. 627. pp. 58-66.
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