Adult hematopoietic stem cells provide functional hemangioblast activity during retinal neovascularization

Maria B. Grant, W. Stratford May, Sergio Caballero, Gary A J Brown, Steven M. Guthrie, Robert N. Mames, Barry J. Byrne, Timothy Vaught, Polyxenie E. Spoerri, Ammon B. Peck, Edward W. Scott

Research output: Contribution to journalArticle

581 Citations (Scopus)

Abstract

Adults maintain a reservoir of hematopoietic stem cells that can enter the circulation to reach organs in need of regeneration. We developed a novel model of retinal neovascularization in adult mice to examine the role of hematopoietic stem cells in revascularizing ischemic retinas. Adult mice were durably engrafted with hematopoietic stem cells isolated from transgenic mice expressing green fluorescent protein. We performed serial long-term transplants, to ensure activity arose from self-renewing stem cells, and single hematopoietic stem-cell transplants to show clonality. After durable hematopoietic engraftment was established, retinal ischemia was induced to promote neovascularization. Our results indicate that self-renewing adult hematopoietic stem cells have functional hemangioblast activity, that is, they can clonally differentiate into all hematopoietic cell lineages as well as endothelial cells that revascularize adult retina. We also show that recruitment of endothelial precursors to sites of ischemic injury has a significant role in neovascularization.

Original languageEnglish (US)
Pages (from-to)607-612
Number of pages6
JournalNature Medicine
Volume8
Issue number6
DOIs
StatePublished - 2002
Externally publishedYes

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Hemangioblasts
Retinal Neovascularization
Adult Stem Cells
Hematopoietic Stem Cells
Stem cells
Transplants
Retina
Cell Lineage
Green Fluorescent Proteins
Endothelial cells
Transgenic Mice
Regeneration
Stem Cells
Ischemia
Endothelial Cells
Wounds and Injuries

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Grant, M. B., May, W. S., Caballero, S., Brown, G. A. J., Guthrie, S. M., Mames, R. N., ... Scott, E. W. (2002). Adult hematopoietic stem cells provide functional hemangioblast activity during retinal neovascularization. Nature Medicine, 8(6), 607-612. https://doi.org/10.1038/nm0602-607

Adult hematopoietic stem cells provide functional hemangioblast activity during retinal neovascularization. / Grant, Maria B.; May, W. Stratford; Caballero, Sergio; Brown, Gary A J; Guthrie, Steven M.; Mames, Robert N.; Byrne, Barry J.; Vaught, Timothy; Spoerri, Polyxenie E.; Peck, Ammon B.; Scott, Edward W.

In: Nature Medicine, Vol. 8, No. 6, 2002, p. 607-612.

Research output: Contribution to journalArticle

Grant, MB, May, WS, Caballero, S, Brown, GAJ, Guthrie, SM, Mames, RN, Byrne, BJ, Vaught, T, Spoerri, PE, Peck, AB & Scott, EW 2002, 'Adult hematopoietic stem cells provide functional hemangioblast activity during retinal neovascularization', Nature Medicine, vol. 8, no. 6, pp. 607-612. https://doi.org/10.1038/nm0602-607
Grant, Maria B. ; May, W. Stratford ; Caballero, Sergio ; Brown, Gary A J ; Guthrie, Steven M. ; Mames, Robert N. ; Byrne, Barry J. ; Vaught, Timothy ; Spoerri, Polyxenie E. ; Peck, Ammon B. ; Scott, Edward W. / Adult hematopoietic stem cells provide functional hemangioblast activity during retinal neovascularization. In: Nature Medicine. 2002 ; Vol. 8, No. 6. pp. 607-612.
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