Adult human CD133/1+ kidney cells isolated from papilla integrate into developing kidney tubules

Heather H. Ward, Elsa Romero, Angela Welford, Gavin Pickett, Robert Bacallao, Vincent H. Gattone, Scott A. Ness, Angela Wandinger-Ness, Tamara Roitbak

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Approximately 60,000 patients in the United States are waiting for a kidney transplant due to genetic, immunologic and environmentally caused kidney failure. Adult human renal stem cells could offer opportunities for autologous transplant and repair of damaged organs. Current data suggest that there are multiple progenitor types in the kidney with distinct localizations. In the present study, we characterize cells derived from human kidney papilla and show their capacity for tubulogenesis. In situ, nestin+ and CD133/1+ cells were found extensively intercalated between tubular epithelia in the loops of Henle of renal papilla, but not of the cortex. Populations of primary cells from the renal cortex and renal papilla were isolated by enzymatic digestion from human kidneys unsuited for transplant and immuno-enriched for CD133/1+ cells. Isolated CD133/1+ papillary cells were positive for nestin, as well as several human embryonic stem cell markers (SSEA4, Nanog, SOX2, and OCT4/POU5F1) and could be triggered to adopt tubular epithelial and neuronal-like phenotypes. Isolated papillary cells exhibited morphologic plasticity upon modulation of culture conditions and inhibition of asymmetric cell division. Labeled papillary cells readily associated with cortical tubular epithelia in co-culture and 3-dimensional collagen gel cultures. Heterologous organ culture demonstrated that CD133/1+ progenitors from the papilla and cortex became integrated into developing kidney tubules. Tubular epithelia did not participate in tubulogenesis. Human renal papilla harbor cells with the hallmarks of adult kidney stem/progenitor cells that can be amplified and phenotypically modulated in culture while retaining the capacity to form new kidney tubules.

Original languageEnglish
Pages (from-to)1344-1357
Number of pages14
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1812
Issue number10
DOIs
StatePublished - Oct 2011

Fingerprint

Kidney Tubules
Kidney
Nestin
Stem Cells
Epithelium
Asymmetric Cell Division
Kidney Medulla
Transplants
Loop of Henle
Organ Culture Techniques
Autografts
Coculture Techniques
Renal Insufficiency
Digestion
Collagen
Gels
Phenotype

Keywords

  • ADPKD
  • Kidney disease
  • Mesenchymal stem cell
  • Metanephric organ culture
  • Regenerative medicine
  • Renopoietic
  • Tamm-Horsfall/uromodulin
  • Xanthosine

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine

Cite this

Adult human CD133/1+ kidney cells isolated from papilla integrate into developing kidney tubules. / Ward, Heather H.; Romero, Elsa; Welford, Angela; Pickett, Gavin; Bacallao, Robert; Gattone, Vincent H.; Ness, Scott A.; Wandinger-Ness, Angela; Roitbak, Tamara.

In: Biochimica et Biophysica Acta - Molecular Basis of Disease, Vol. 1812, No. 10, 10.2011, p. 1344-1357.

Research output: Contribution to journalArticle

Ward, HH, Romero, E, Welford, A, Pickett, G, Bacallao, R, Gattone, VH, Ness, SA, Wandinger-Ness, A & Roitbak, T 2011, 'Adult human CD133/1+ kidney cells isolated from papilla integrate into developing kidney tubules', Biochimica et Biophysica Acta - Molecular Basis of Disease, vol. 1812, no. 10, pp. 1344-1357. https://doi.org/10.1016/j.bbadis.2011.01.010
Ward, Heather H. ; Romero, Elsa ; Welford, Angela ; Pickett, Gavin ; Bacallao, Robert ; Gattone, Vincent H. ; Ness, Scott A. ; Wandinger-Ness, Angela ; Roitbak, Tamara. / Adult human CD133/1+ kidney cells isolated from papilla integrate into developing kidney tubules. In: Biochimica et Biophysica Acta - Molecular Basis of Disease. 2011 ; Vol. 1812, No. 10. pp. 1344-1357.
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