Advanced age-related denervation and fiber-type grouping in skeletal muscle of SOD1 knockout mice

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In this study skeletal muscles from 1.5- and 10-month-old Cu/Zn superoxide dismutase (SOD1) homozygous knockout (JLSod1-/-) mice obtained from The Jackson Laboratory (C57Bl6/129SvEv background) were compared with muscles from age- and sex-matched heterozygous (JLSod1+/-) littermates. The results of this study were compared with previously published data on two different strains of Sod1-/- mice: one from Dr. Epstein's laboratory (ELSod1-/-; C57Bl6 background) and the other from Cephalon, Inc. (CSod1-/-; 129/CD-1 background). Grouping of succinate dehydrogenase-positive fibers characterized muscles of Sod1-/- mice from all three strains. The 10-month-old Sod1-/- C and JL mice displayed pronounced denervation of the gastrocnemius muscle, whereas the ELSod1-/- mice displayed a small degree of denervation at this age, but developed accelerated age-related denervation later on. Denervation markers were up-regulated in skeletal muscle of 10-month-old JLSod1-/- mice. This study is the first to show that metallothionein mRNA and protein expression was up-regulated in the skeletal muscle of 10-month-old JLSod1-/- mice and was mostly localized to the small atrophic muscle fibers. In conclusion, all three strains of Sod1-/- mice develop accelerated age-related muscle denervation, but the genetic background has significant influence on the progress of denervation.

Original languageEnglish (US)
Pages (from-to)1582-1593
Number of pages12
JournalFree Radical Biology and Medicine
Volume49
Issue number10
DOIs
StatePublished - Nov 30 2010

Fingerprint

Denervation
Knockout Mice
Muscle
Skeletal Muscle
Fibers
Muscles
Muscle Denervation
Succinate Dehydrogenase
Metallothionein
Messenger RNA
Proteins

Keywords

  • Aging
  • Denervation
  • Fiber-type grouping
  • Free radicals
  • Skeletal muscle
  • Superoxide dismutase

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Advanced age-related denervation and fiber-type grouping in skeletal muscle of SOD1 knockout mice. / Kostrominova, Tatiana.

In: Free Radical Biology and Medicine, Vol. 49, No. 10, 30.11.2010, p. 1582-1593.

Research output: Contribution to journalArticle

@article{da7fcdedd6d44fbc91680bb13b26b9b3,
title = "Advanced age-related denervation and fiber-type grouping in skeletal muscle of SOD1 knockout mice",
abstract = "In this study skeletal muscles from 1.5- and 10-month-old Cu/Zn superoxide dismutase (SOD1) homozygous knockout (JLSod1-/-) mice obtained from The Jackson Laboratory (C57Bl6/129SvEv background) were compared with muscles from age- and sex-matched heterozygous (JLSod1+/-) littermates. The results of this study were compared with previously published data on two different strains of Sod1-/- mice: one from Dr. Epstein's laboratory (ELSod1-/-; C57Bl6 background) and the other from Cephalon, Inc. (CSod1-/-; 129/CD-1 background). Grouping of succinate dehydrogenase-positive fibers characterized muscles of Sod1-/- mice from all three strains. The 10-month-old Sod1-/- C and JL mice displayed pronounced denervation of the gastrocnemius muscle, whereas the ELSod1-/- mice displayed a small degree of denervation at this age, but developed accelerated age-related denervation later on. Denervation markers were up-regulated in skeletal muscle of 10-month-old JLSod1-/- mice. This study is the first to show that metallothionein mRNA and protein expression was up-regulated in the skeletal muscle of 10-month-old JLSod1-/- mice and was mostly localized to the small atrophic muscle fibers. In conclusion, all three strains of Sod1-/- mice develop accelerated age-related muscle denervation, but the genetic background has significant influence on the progress of denervation.",
keywords = "Aging, Denervation, Fiber-type grouping, Free radicals, Skeletal muscle, Superoxide dismutase",
author = "Tatiana Kostrominova",
year = "2010",
month = "11",
day = "30",
doi = "10.1016/j.freeradbiomed.2010.08.022",
language = "English (US)",
volume = "49",
pages = "1582--1593",
journal = "Free Radical Biology and Medicine",
issn = "0891-5849",
publisher = "Elsevier Inc.",
number = "10",

}

TY - JOUR

T1 - Advanced age-related denervation and fiber-type grouping in skeletal muscle of SOD1 knockout mice

AU - Kostrominova, Tatiana

PY - 2010/11/30

Y1 - 2010/11/30

N2 - In this study skeletal muscles from 1.5- and 10-month-old Cu/Zn superoxide dismutase (SOD1) homozygous knockout (JLSod1-/-) mice obtained from The Jackson Laboratory (C57Bl6/129SvEv background) were compared with muscles from age- and sex-matched heterozygous (JLSod1+/-) littermates. The results of this study were compared with previously published data on two different strains of Sod1-/- mice: one from Dr. Epstein's laboratory (ELSod1-/-; C57Bl6 background) and the other from Cephalon, Inc. (CSod1-/-; 129/CD-1 background). Grouping of succinate dehydrogenase-positive fibers characterized muscles of Sod1-/- mice from all three strains. The 10-month-old Sod1-/- C and JL mice displayed pronounced denervation of the gastrocnemius muscle, whereas the ELSod1-/- mice displayed a small degree of denervation at this age, but developed accelerated age-related denervation later on. Denervation markers were up-regulated in skeletal muscle of 10-month-old JLSod1-/- mice. This study is the first to show that metallothionein mRNA and protein expression was up-regulated in the skeletal muscle of 10-month-old JLSod1-/- mice and was mostly localized to the small atrophic muscle fibers. In conclusion, all three strains of Sod1-/- mice develop accelerated age-related muscle denervation, but the genetic background has significant influence on the progress of denervation.

AB - In this study skeletal muscles from 1.5- and 10-month-old Cu/Zn superoxide dismutase (SOD1) homozygous knockout (JLSod1-/-) mice obtained from The Jackson Laboratory (C57Bl6/129SvEv background) were compared with muscles from age- and sex-matched heterozygous (JLSod1+/-) littermates. The results of this study were compared with previously published data on two different strains of Sod1-/- mice: one from Dr. Epstein's laboratory (ELSod1-/-; C57Bl6 background) and the other from Cephalon, Inc. (CSod1-/-; 129/CD-1 background). Grouping of succinate dehydrogenase-positive fibers characterized muscles of Sod1-/- mice from all three strains. The 10-month-old Sod1-/- C and JL mice displayed pronounced denervation of the gastrocnemius muscle, whereas the ELSod1-/- mice displayed a small degree of denervation at this age, but developed accelerated age-related denervation later on. Denervation markers were up-regulated in skeletal muscle of 10-month-old JLSod1-/- mice. This study is the first to show that metallothionein mRNA and protein expression was up-regulated in the skeletal muscle of 10-month-old JLSod1-/- mice and was mostly localized to the small atrophic muscle fibers. In conclusion, all three strains of Sod1-/- mice develop accelerated age-related muscle denervation, but the genetic background has significant influence on the progress of denervation.

KW - Aging

KW - Denervation

KW - Fiber-type grouping

KW - Free radicals

KW - Skeletal muscle

KW - Superoxide dismutase

UR - http://www.scopus.com/inward/record.url?scp=77957749827&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957749827&partnerID=8YFLogxK

U2 - 10.1016/j.freeradbiomed.2010.08.022

DO - 10.1016/j.freeradbiomed.2010.08.022

M3 - Article

VL - 49

SP - 1582

EP - 1593

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

SN - 0891-5849

IS - 10

ER -