Age and sex-dependent decreases in ChAT in basal forebrain nuclei

Victoria N. Luine, Kenneth J. Renner, Sarah Heady, Kathryn Jones

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Microdissection techniques were utilized to measure the activity of choline acetyltransferase (ChAT) (enzyme responsible for synthesis of acetylcholine) in individual basal forebrain nuclei of aged (24 month) and young (4 month) male and female rats. Small but consistent decreases in the activity of ChAT in aged rats were found, and the location of the changes was dependent on the sex of the rat. Aged female rats showed approximately 30% lower ChAT and 40% lower acetylcholinesterase (AChE) activity in the ventral globus pallidus (vGP). Aged males did not show decreased ChAT in the vGP but activity in the medial aspect of the horizontal diagonal band nucleus was 50% lower than in the young males. ChAT activity in four other closely aligned basal forebrain nuclei was not different betwen the young and aged rats. Analysis of cell number, density and area in the vGP by AChE histochemistry showed no significant differences between aged and young females. In addition, age and sex-dependent changes were measured in pituitary glucose-6-phosphate dehydrogenase activity. The relationship of the changes to age-dependent decrements in memory, the possible influence of gonadal hormones on aging, and the mechanisms responsible for age-related declines in ChAT activity are discussed.

Original languageEnglish (US)
Pages (from-to)193-198
Number of pages6
JournalNeurobiology of Aging
Volume7
Issue number3
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Choline O-Acetyltransferase
Basal Ganglia
Globus Pallidus
Acetylcholinesterase
Cell Count
Gonadal Hormones
Septal Nuclei
Microdissection
Glucosephosphate Dehydrogenase
Acetylcholine
Basal Forebrain
Enzymes

Keywords

  • AChE
  • Aging
  • Basal forebrain
  • ChAT

ASJC Scopus subject areas

  • Clinical Neurology
  • Biological Psychiatry
  • Developmental Neuroscience
  • Neurology
  • Psychology(all)

Cite this

Age and sex-dependent decreases in ChAT in basal forebrain nuclei. / Luine, Victoria N.; Renner, Kenneth J.; Heady, Sarah; Jones, Kathryn.

In: Neurobiology of Aging, Vol. 7, No. 3, 1986, p. 193-198.

Research output: Contribution to journalArticle

Luine, Victoria N. ; Renner, Kenneth J. ; Heady, Sarah ; Jones, Kathryn. / Age and sex-dependent decreases in ChAT in basal forebrain nuclei. In: Neurobiology of Aging. 1986 ; Vol. 7, No. 3. pp. 193-198.
@article{dfeaba0539a941f8be227a753bf7f00f,
title = "Age and sex-dependent decreases in ChAT in basal forebrain nuclei",
abstract = "Microdissection techniques were utilized to measure the activity of choline acetyltransferase (ChAT) (enzyme responsible for synthesis of acetylcholine) in individual basal forebrain nuclei of aged (24 month) and young (4 month) male and female rats. Small but consistent decreases in the activity of ChAT in aged rats were found, and the location of the changes was dependent on the sex of the rat. Aged female rats showed approximately 30{\%} lower ChAT and 40{\%} lower acetylcholinesterase (AChE) activity in the ventral globus pallidus (vGP). Aged males did not show decreased ChAT in the vGP but activity in the medial aspect of the horizontal diagonal band nucleus was 50{\%} lower than in the young males. ChAT activity in four other closely aligned basal forebrain nuclei was not different betwen the young and aged rats. Analysis of cell number, density and area in the vGP by AChE histochemistry showed no significant differences between aged and young females. In addition, age and sex-dependent changes were measured in pituitary glucose-6-phosphate dehydrogenase activity. The relationship of the changes to age-dependent decrements in memory, the possible influence of gonadal hormones on aging, and the mechanisms responsible for age-related declines in ChAT activity are discussed.",
keywords = "AChE, Aging, Basal forebrain, ChAT",
author = "Luine, {Victoria N.} and Renner, {Kenneth J.} and Sarah Heady and Kathryn Jones",
year = "1986",
doi = "10.1016/0197-4580(86)90042-4",
language = "English (US)",
volume = "7",
pages = "193--198",
journal = "Neurobiology of Aging",
issn = "0197-4580",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Age and sex-dependent decreases in ChAT in basal forebrain nuclei

AU - Luine, Victoria N.

AU - Renner, Kenneth J.

AU - Heady, Sarah

AU - Jones, Kathryn

PY - 1986

Y1 - 1986

N2 - Microdissection techniques were utilized to measure the activity of choline acetyltransferase (ChAT) (enzyme responsible for synthesis of acetylcholine) in individual basal forebrain nuclei of aged (24 month) and young (4 month) male and female rats. Small but consistent decreases in the activity of ChAT in aged rats were found, and the location of the changes was dependent on the sex of the rat. Aged female rats showed approximately 30% lower ChAT and 40% lower acetylcholinesterase (AChE) activity in the ventral globus pallidus (vGP). Aged males did not show decreased ChAT in the vGP but activity in the medial aspect of the horizontal diagonal band nucleus was 50% lower than in the young males. ChAT activity in four other closely aligned basal forebrain nuclei was not different betwen the young and aged rats. Analysis of cell number, density and area in the vGP by AChE histochemistry showed no significant differences between aged and young females. In addition, age and sex-dependent changes were measured in pituitary glucose-6-phosphate dehydrogenase activity. The relationship of the changes to age-dependent decrements in memory, the possible influence of gonadal hormones on aging, and the mechanisms responsible for age-related declines in ChAT activity are discussed.

AB - Microdissection techniques were utilized to measure the activity of choline acetyltransferase (ChAT) (enzyme responsible for synthesis of acetylcholine) in individual basal forebrain nuclei of aged (24 month) and young (4 month) male and female rats. Small but consistent decreases in the activity of ChAT in aged rats were found, and the location of the changes was dependent on the sex of the rat. Aged female rats showed approximately 30% lower ChAT and 40% lower acetylcholinesterase (AChE) activity in the ventral globus pallidus (vGP). Aged males did not show decreased ChAT in the vGP but activity in the medial aspect of the horizontal diagonal band nucleus was 50% lower than in the young males. ChAT activity in four other closely aligned basal forebrain nuclei was not different betwen the young and aged rats. Analysis of cell number, density and area in the vGP by AChE histochemistry showed no significant differences between aged and young females. In addition, age and sex-dependent changes were measured in pituitary glucose-6-phosphate dehydrogenase activity. The relationship of the changes to age-dependent decrements in memory, the possible influence of gonadal hormones on aging, and the mechanisms responsible for age-related declines in ChAT activity are discussed.

KW - AChE

KW - Aging

KW - Basal forebrain

KW - ChAT

UR - http://www.scopus.com/inward/record.url?scp=0022922249&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022922249&partnerID=8YFLogxK

U2 - 10.1016/0197-4580(86)90042-4

DO - 10.1016/0197-4580(86)90042-4

M3 - Article

VL - 7

SP - 193

EP - 198

JO - Neurobiology of Aging

JF - Neurobiology of Aging

SN - 0197-4580

IS - 3

ER -