Age-associated hydroxymethylation in human bone-marrow mesenchymal stem cells

Estela G. Toraño, Gustavo F. Bayón, Álvaro del Real, Marta I. Sierra, María G. García, Antonella Carella, Thalia Belmonte, Rocío G. Urdinguio, Isabel Cubillo, Javier García-Castro, Jesús Delgado-Calle, Flor M. Pérez-Campo, José A. Riancho, Mario F. Fraga, Agustín F. Fernández

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Background: Age-associated changes in genomic DNA methylation have been primarily attributed to 5-methylcytosine (5mC). However, the recent discovery of 5-hydroxymethylcytosine (5hmC) suggests that this epigenetic mark might also play a role in the process. Methods: Here, we analyzed the genome-wide profile of 5hmc in mesenchymal stem cells (MSCs) obtained from bone-marrow donors, aged 2-89 years. Results: We identified 10,685 frequently hydroxymethylated CpG sites in MSCs that were, as in other cell types, significantly associated with low density CpG regions, introns, the histone posttranslational modification H3k4me1 and enhancers. Study of the age-associated changes to 5hmC identified 785 hyper- and 846 hypo-hydroxymethylated CpG sites in the MSCs obtained from older individuals. Conclusions: DNA hyper-hydroxymethylation in the advanced-age group was associated with loss of 5mC, which suggests that, at specific CpG sites, this epigenetic modification might play a role in DNA methylation changes during lifetime. Since bone-marrow MSCs have many clinical applications, and the fact that the epigenomic alterations in this cell type associated with aging identified in this study could have associated functional effects, the age of donors should be taken into account in clinical settings.

Original languageEnglish (US)
Article number207
JournalJournal of Translational Medicine
Volume14
Issue number1
DOIs
StatePublished - 2016

Keywords

  • 5hmC
  • Aging
  • Bone-marrow
  • Epigenetics
  • MSCs

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Toraño, E. G., Bayón, G. F., del Real, Á., Sierra, M. I., García, M. G., Carella, A., Belmonte, T., Urdinguio, R. G., Cubillo, I., García-Castro, J., Delgado-Calle, J., Pérez-Campo, F. M., Riancho, J. A., Fraga, M. F., & Fernández, A. F. (2016). Age-associated hydroxymethylation in human bone-marrow mesenchymal stem cells. Journal of Translational Medicine, 14(1), [207]. https://doi.org/10.1186/s12967-016-0966-x