Age-related changes in structure and extracellular matrix protein expression levels in rat tendons

Tatiana Kostrominova, Susan V. Brooks

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The musculoskeletal system (muscle-tendon-bone) demonstrates numerous age-related changes, with modifications in tendons the least well studied, although increased predisposition to tendinopathy and rupture have been reported. In order to gain insights into the basis of age-associated increase in tendon injuries, we compared Achilles and tibialis anterior tendons and myotendinous junctions (MTJs) from 3- to 5- and 22-to 25-month-old rats for underlying structure and composition. Significant decreases were observed by qRTPCR for collagen I, III, and V mRNA expression in tendons of old rats, but immunostaining detected no apparent differences in collagen I and V expression on the protein level. Tendons of old compared with youngrats had decreased mRNA expression levels of proteoglycan 4 (PRG4) and elastin (Eln), but no differences in the mRNA expression of connective tissue growth factor, TGF-beta 1, or stromal cell-derived factor 1. For PRG4, immunostaining showed good correlation with qRT-PCR results. This is the first study to show reductions in PRG4 in tendons and MTJs of old rats. Decreased PRG4 expression in tendons could result in increased tendon stiffness and may be associated with decreased activity in the elderly. The diminished collagen mRNA expression in combination with decreased PRG4 and Eln mRNA expression may be associated with increased risk of tendon injury with aging.

Original languageEnglish (US)
Pages (from-to)2203-2214
Number of pages12
JournalAge
Volume35
Issue number6
DOIs
StatePublished - Dec 2013

Fingerprint

Extracellular Matrix Proteins
Tendons
Proteoglycans
Messenger RNA
Tendon Injuries
Collagen
Elastin
Connective Tissue Growth Factor
Chemokine CXCL12
Transforming Growth Factor beta1
Musculoskeletal System
Tendinopathy
Rupture
Bone and Bones
Muscles
Polymerase Chain Reaction

Keywords

  • Aging
  • Elastin
  • Myotendinous junctions
  • PRG4
  • Tendon

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

Cite this

Age-related changes in structure and extracellular matrix protein expression levels in rat tendons. / Kostrominova, Tatiana; Brooks, Susan V.

In: Age, Vol. 35, No. 6, 12.2013, p. 2203-2214.

Research output: Contribution to journalArticle

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