Age-related changes in the mechanical properties of the epimysium in skeletal muscles of rats

Yingxin Gao, Tatiana Kostrominova, John A. Faulkner, Alan S. Wineman

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Skeletal muscle is composed of muscle fibers and an extracellular matrix (ECM). The collagen fiber network of the ECM is a major contributor to the passive force of skeletal muscles at high strain. We investigated the effect of aging on the biomechanical and structural properties of epimysium of the tibialis anterior muscles (TBA) of rats to understand the mechanisms responsible for the age-related changes. The biomechanical properties were tested directly in vitro by uniaxial extension of epimysium. The presence of age-related changes in the arrangement and size of the collagen fibrils in the epimysium was examined by scanning electron microscopy (SEM). A mathematical model was subsequently developed based on the structure-function relationships that predicted the compliance of the epimysium. Biomechanically, the epimysium from old rats was much stiffer than that of the young rats. No differences were found in the ultrastructure and thickness of the epimysium or size of the collagen fibrils between young and old rats. The changes in the arrangement and size of the collagen fibrils do not appear to be the principal cause of the increased stiffness of the epimysium from the old rats. Other changes in the structural composition of the epimysium from old rats likely has a strong effect on the increased stiffness. The age-related increase in the stiffness of the epimysium could play an important role in the impaired lateral force transmission in the muscles of the elderly.

Original languageEnglish (US)
Pages (from-to)465-469
Number of pages5
JournalJournal of Biomechanics
Volume41
Issue number2
DOIs
StatePublished - 2008

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Muscle
Rats
Skeletal Muscle
Collagen
Mechanical properties
Stiffness
Muscles
Extracellular Matrix
Fibers
Electron Scanning Microscopy
Compliance
Structural properties
Theoretical Models
Aging of materials
Mathematical models
Scanning electron microscopy
Chemical analysis

Keywords

  • Collagen fibrils
  • Epimysium
  • Mathematical modeling
  • Mechanical properties
  • Scanning electron microscopy (SEM)
  • Tibialis anterior muscle (TBA)

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Age-related changes in the mechanical properties of the epimysium in skeletal muscles of rats. / Gao, Yingxin; Kostrominova, Tatiana; Faulkner, John A.; Wineman, Alan S.

In: Journal of Biomechanics, Vol. 41, No. 2, 2008, p. 465-469.

Research output: Contribution to journalArticle

Gao, Yingxin ; Kostrominova, Tatiana ; Faulkner, John A. ; Wineman, Alan S. / Age-related changes in the mechanical properties of the epimysium in skeletal muscles of rats. In: Journal of Biomechanics. 2008 ; Vol. 41, No. 2. pp. 465-469.
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