Aging in heterozygous Dnmt1-deficient mice: Effects on survival, the DNA methylation genes, and the development of amyloidosis

Donna Ray, Ailing Wu, J. Erby Wilkinson, Hedwig S. Murphy, Qianjin Lu, Barbara Kluve-Beckerman, Juris J. Liepnieks, Merrill Benson, Raymond Yung, Bruce Richardson

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

We previously reported that heterozygous DNA methyltransferase 1-deficient (Dnmt1+/-) mice maintain T-cell immune function and DNA methylation levels with aging, whereas controls develop autoimmunity, immune senescence, and DNA hypomethylation. We therefore compared survival, cause of death, and T-cell DNA methylation gene expression during aging in Dnmt+/- mice and controls. No difference in longevity was observed, but greater numbers of Dnmt1+/- mice developed jejunal apolipoprotein AII amyloidosis. Both groups showed decreased Dnmt1 expression with aging. However, expression of the de novo methyltransferases Dnmt3a and Dnmt3b increased with aging in stimulated T cells from control mice. MeCP2, a methylcytosine binding protein that participates in maintenance DNA methylation, increased with age in Dnmt1 +/- mice, suggesting a mechanism for the sustained DNA methylation levels. This model thus provides potential mechanisms for DNA methylation changes of aging, and suggests that changes in DNA methylation may contribute to some forms of amyloidosis that develop with aging.

Original languageEnglish (US)
Pages (from-to)115-124
Number of pages10
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume61
Issue number2
DOIs
StatePublished - Feb 2006

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

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