Agonist-driven development of CD4+CD25+Foxp3 + regulatory T cells requires a second signal mediated by Stat6

Vanesa Sanchez-Guajardo, Corinne Tanchot, John T. O'Malley, Mark Kaplan, Sylvie Garcia, Antonio A. Freitas

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The factors that induce Foxp3 expression and regulatory T (Treg) cell development remain unknown. In this study, we investigated the role of STAT4 and STAT6 in agonist-driven generation of Ag-specific Foxp3-expressing Treg cells. Our findings indicate that fully efficient induction of Foxp3 expression and development of Ag-specific Treg cells requires the synergistic action of two signals: a TCR-mediated signal and a second signal mediated by STAT6. Indeed, by comparing the development of wild-type and STAT4- and STAT6-deficient hemagglutinin-specific T cells in the presence of hemagglutinin Ag, we found that the absence of STAT6 impaired the generation of Ag-specific CD4 +CD25+Foxp3+ cells. Moreover, in transgenic mice expressing a constitutively active form of STAT6, we found that the fraction of CD4+Foxp3+ cells exceeds that of control wild-type littermates. Overall these findings support a role for the STAT6 pathway in Treg cell development and maintenance.

Original languageEnglish
Pages (from-to)7550-7556
Number of pages7
JournalJournal of Immunology
Volume178
Issue number12
StatePublished - Jun 15 2007

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Regulatory T-Lymphocytes
Hemagglutinins
Transgenic Mice
Maintenance
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Sanchez-Guajardo, V., Tanchot, C., O'Malley, J. T., Kaplan, M., Garcia, S., & Freitas, A. A. (2007). Agonist-driven development of CD4+CD25+Foxp3 + regulatory T cells requires a second signal mediated by Stat6. Journal of Immunology, 178(12), 7550-7556.

Agonist-driven development of CD4+CD25+Foxp3 + regulatory T cells requires a second signal mediated by Stat6. / Sanchez-Guajardo, Vanesa; Tanchot, Corinne; O'Malley, John T.; Kaplan, Mark; Garcia, Sylvie; Freitas, Antonio A.

In: Journal of Immunology, Vol. 178, No. 12, 15.06.2007, p. 7550-7556.

Research output: Contribution to journalArticle

Sanchez-Guajardo, V, Tanchot, C, O'Malley, JT, Kaplan, M, Garcia, S & Freitas, AA 2007, 'Agonist-driven development of CD4+CD25+Foxp3 + regulatory T cells requires a second signal mediated by Stat6', Journal of Immunology, vol. 178, no. 12, pp. 7550-7556.
Sanchez-Guajardo V, Tanchot C, O'Malley JT, Kaplan M, Garcia S, Freitas AA. Agonist-driven development of CD4+CD25+Foxp3 + regulatory T cells requires a second signal mediated by Stat6. Journal of Immunology. 2007 Jun 15;178(12):7550-7556.
Sanchez-Guajardo, Vanesa ; Tanchot, Corinne ; O'Malley, John T. ; Kaplan, Mark ; Garcia, Sylvie ; Freitas, Antonio A. / Agonist-driven development of CD4+CD25+Foxp3 + regulatory T cells requires a second signal mediated by Stat6. In: Journal of Immunology. 2007 ; Vol. 178, No. 12. pp. 7550-7556.
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