AirSR, a [2Fe-2S] cluster-containing two-component system, mediates global oxygen sensing and redox signaling in staphylococcus aureus

Fei Sun, Quanjiang Ji, Marcus B. Jones, Xin Deng, Haihua Liang, Bryan Frank, Joshua Telser, Scott N. Peterson, Taeok Bae, Chuan He

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Oxygen sensing and redox signaling significantly affect bacterial physiology and host-pathogen interaction. Here we show that a Staphylococcus aureus two-component system, AirSR (anaerobic iron-sulfur cluster-containing redox sensor regulator, formerly YhcSR), responds to oxidation signals (O 2, H 2O 2, NO, etc) by using a redox-active [2Fe-2S] cluster in the sensor kinase AirS. Mutagenesis studies demonstrate that the [2Fe-2S] cluster is essential for the kinase activity of AirS. We have also discovered that a homologue of IscS (SA1450) in S. aureus is active as a cysteine desulfurase, which enables the in vitro reconstitution of the [2Fe-2S] cluster in AirS. Phosphorylation assays show that the oxidized AirS with a [2Fe-2S] 2+ cluster is the fully active form of the kinase but not the apo-AirS nor the reduced AirS possessing a [2Fe-2S] + cluster. Overoxidation by prolonged exposure to O 2 or contact with H 2O 2 or NO led to inactivation of AirS. Transcriptome analysis revealed that mutation of airR impacts the expression of ∼355 genes under anaerobic conditions. Moreover, the mutant strain displayed increased resistance toward H 2O 2, vancomycin, norfloxacin, and ciprofloxacin under anaerobic conditions. Together, our results show that S. aureus AirSR is a redox-dependent global regulatory system that plays important roles in gene regulation using a redox active Fe-S cluster under O 2-limited conditions.

Original languageEnglish
Pages (from-to)305-314
Number of pages10
JournalJournal of the American Chemical Society
Volume134
Issue number1
DOIs
StatePublished - Jan 11 2012

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Oxidation-Reduction
Staphylococcus aureus
Air
Oxygen
Phosphotransferases
Bacterial Physiological Phenomena
Host-Pathogen Interactions
Norfloxacin
Mutagenesis
Phosphorylation
Sensors
Physiology
Gene Expression Profiling
Pathogens
Vancomycin
Ciprofloxacin
Sulfur
Gene expression
Assays
Iron

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

AirSR, a [2Fe-2S] cluster-containing two-component system, mediates global oxygen sensing and redox signaling in staphylococcus aureus. / Sun, Fei; Ji, Quanjiang; Jones, Marcus B.; Deng, Xin; Liang, Haihua; Frank, Bryan; Telser, Joshua; Peterson, Scott N.; Bae, Taeok; He, Chuan.

In: Journal of the American Chemical Society, Vol. 134, No. 1, 11.01.2012, p. 305-314.

Research output: Contribution to journalArticle

Sun, Fei ; Ji, Quanjiang ; Jones, Marcus B. ; Deng, Xin ; Liang, Haihua ; Frank, Bryan ; Telser, Joshua ; Peterson, Scott N. ; Bae, Taeok ; He, Chuan. / AirSR, a [2Fe-2S] cluster-containing two-component system, mediates global oxygen sensing and redox signaling in staphylococcus aureus. In: Journal of the American Chemical Society. 2012 ; Vol. 134, No. 1. pp. 305-314.
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