AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon

M. J. Zinda, M. A. Johnson, J. D. Paul, C. Horn, B. W. Konicek, Hai Lu Zhao Hai Lu, George Sandusky, J. E. Thomas, B. L. Neubauer, M. T. Lai, J. R. Graff

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

Purpose: The AKT/PKB kinase controls many of the intracellular processes that are dysregulated in human cancer, including the suppression of apoptosis and anoikis and the induction of cell cycle progression. Three isoforms of AKT have been identified: AKT-1, -2, and -3. Selective up-regulation of AKT-3 RNA expression has been reported in hormone-independent breast and prostate cancer cell lines suggesting that AKT-3 expression may be increased with breast or prostate tumor progression. To determine whether AKT-3 RNA expression is selectively up-regulated in human cancers and whether the patterns of AKT RNA expression may change with tumor development, we examined AKT isoform expression by RT-PCR in human cancer cell lines, primary human cancers, and normal human tissues. Experimental Design: AKT-1, -2, and -3 RNA expression was examined by RT-PCR. Because up-regulated AKT-3 expression has been implicated in human breast and prostate cancer progression, we also examined AKT-3 expression levels by semiquantitative RT-PCR using matched normal/tumor first-strand cDNA pairs from colon, breast, prostate, and lung cancers. Results: Our data reveal that the overwhelming majority of both normal and tumor tissues express all three of the AKT isoforms. Moreover, semiquantitative RT-PCR of matched normal/tumor pairs confirmed similar AKT-3 RNA expression levels in both normal and tumor tissue. Conclusions: Our data show that both normal and tumor tissues express all three of the AKT isoforms and indicate that tumorigenesis does not involve a dramatic shift in the RNA expression patterns of the three AKT isoforms.

Original languageEnglish (US)
Pages (from-to)2475-2479
Number of pages5
JournalClinical Cancer Research
Volume7
Issue number8
StatePublished - 2001
Externally publishedYes

Fingerprint

Prostate
Colon
Breast
Lung
Neoplasms
RNA
Protein Isoforms
Polymerase Chain Reaction
Prostatic Neoplasms
Breast Neoplasms
Anoikis
Cell Line
Colonic Neoplasms
Lung Neoplasms
Cell Cycle
Carcinogenesis
Research Design
Phosphotransferases
Up-Regulation
Complementary DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Zinda, M. J., Johnson, M. A., Paul, J. D., Horn, C., Konicek, B. W., Zhao Hai Lu, H. L., ... Graff, J. R. (2001). AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon. Clinical Cancer Research, 7(8), 2475-2479.

AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon. / Zinda, M. J.; Johnson, M. A.; Paul, J. D.; Horn, C.; Konicek, B. W.; Zhao Hai Lu, Hai Lu; Sandusky, George; Thomas, J. E.; Neubauer, B. L.; Lai, M. T.; Graff, J. R.

In: Clinical Cancer Research, Vol. 7, No. 8, 2001, p. 2475-2479.

Research output: Contribution to journalArticle

Zinda, MJ, Johnson, MA, Paul, JD, Horn, C, Konicek, BW, Zhao Hai Lu, HL, Sandusky, G, Thomas, JE, Neubauer, BL, Lai, MT & Graff, JR 2001, 'AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon', Clinical Cancer Research, vol. 7, no. 8, pp. 2475-2479.
Zinda MJ, Johnson MA, Paul JD, Horn C, Konicek BW, Zhao Hai Lu HL et al. AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon. Clinical Cancer Research. 2001;7(8):2475-2479.
Zinda, M. J. ; Johnson, M. A. ; Paul, J. D. ; Horn, C. ; Konicek, B. W. ; Zhao Hai Lu, Hai Lu ; Sandusky, George ; Thomas, J. E. ; Neubauer, B. L. ; Lai, M. T. ; Graff, J. R. / AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon. In: Clinical Cancer Research. 2001 ; Vol. 7, No. 8. pp. 2475-2479.
@article{a42cfd4140af4260a11b611f98b9b7a9,
title = "AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon",
abstract = "Purpose: The AKT/PKB kinase controls many of the intracellular processes that are dysregulated in human cancer, including the suppression of apoptosis and anoikis and the induction of cell cycle progression. Three isoforms of AKT have been identified: AKT-1, -2, and -3. Selective up-regulation of AKT-3 RNA expression has been reported in hormone-independent breast and prostate cancer cell lines suggesting that AKT-3 expression may be increased with breast or prostate tumor progression. To determine whether AKT-3 RNA expression is selectively up-regulated in human cancers and whether the patterns of AKT RNA expression may change with tumor development, we examined AKT isoform expression by RT-PCR in human cancer cell lines, primary human cancers, and normal human tissues. Experimental Design: AKT-1, -2, and -3 RNA expression was examined by RT-PCR. Because up-regulated AKT-3 expression has been implicated in human breast and prostate cancer progression, we also examined AKT-3 expression levels by semiquantitative RT-PCR using matched normal/tumor first-strand cDNA pairs from colon, breast, prostate, and lung cancers. Results: Our data reveal that the overwhelming majority of both normal and tumor tissues express all three of the AKT isoforms. Moreover, semiquantitative RT-PCR of matched normal/tumor pairs confirmed similar AKT-3 RNA expression levels in both normal and tumor tissue. Conclusions: Our data show that both normal and tumor tissues express all three of the AKT isoforms and indicate that tumorigenesis does not involve a dramatic shift in the RNA expression patterns of the three AKT isoforms.",
author = "Zinda, {M. J.} and Johnson, {M. A.} and Paul, {J. D.} and C. Horn and Konicek, {B. W.} and {Zhao Hai Lu}, {Hai Lu} and George Sandusky and Thomas, {J. E.} and Neubauer, {B. L.} and Lai, {M. T.} and Graff, {J. R.}",
year = "2001",
language = "English (US)",
volume = "7",
pages = "2475--2479",
journal = "Clinical Cancer Research",
issn = "1078-0432",
publisher = "American Association for Cancer Research Inc.",
number = "8",

}

TY - JOUR

T1 - AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon

AU - Zinda, M. J.

AU - Johnson, M. A.

AU - Paul, J. D.

AU - Horn, C.

AU - Konicek, B. W.

AU - Zhao Hai Lu, Hai Lu

AU - Sandusky, George

AU - Thomas, J. E.

AU - Neubauer, B. L.

AU - Lai, M. T.

AU - Graff, J. R.

PY - 2001

Y1 - 2001

N2 - Purpose: The AKT/PKB kinase controls many of the intracellular processes that are dysregulated in human cancer, including the suppression of apoptosis and anoikis and the induction of cell cycle progression. Three isoforms of AKT have been identified: AKT-1, -2, and -3. Selective up-regulation of AKT-3 RNA expression has been reported in hormone-independent breast and prostate cancer cell lines suggesting that AKT-3 expression may be increased with breast or prostate tumor progression. To determine whether AKT-3 RNA expression is selectively up-regulated in human cancers and whether the patterns of AKT RNA expression may change with tumor development, we examined AKT isoform expression by RT-PCR in human cancer cell lines, primary human cancers, and normal human tissues. Experimental Design: AKT-1, -2, and -3 RNA expression was examined by RT-PCR. Because up-regulated AKT-3 expression has been implicated in human breast and prostate cancer progression, we also examined AKT-3 expression levels by semiquantitative RT-PCR using matched normal/tumor first-strand cDNA pairs from colon, breast, prostate, and lung cancers. Results: Our data reveal that the overwhelming majority of both normal and tumor tissues express all three of the AKT isoforms. Moreover, semiquantitative RT-PCR of matched normal/tumor pairs confirmed similar AKT-3 RNA expression levels in both normal and tumor tissue. Conclusions: Our data show that both normal and tumor tissues express all three of the AKT isoforms and indicate that tumorigenesis does not involve a dramatic shift in the RNA expression patterns of the three AKT isoforms.

AB - Purpose: The AKT/PKB kinase controls many of the intracellular processes that are dysregulated in human cancer, including the suppression of apoptosis and anoikis and the induction of cell cycle progression. Three isoforms of AKT have been identified: AKT-1, -2, and -3. Selective up-regulation of AKT-3 RNA expression has been reported in hormone-independent breast and prostate cancer cell lines suggesting that AKT-3 expression may be increased with breast or prostate tumor progression. To determine whether AKT-3 RNA expression is selectively up-regulated in human cancers and whether the patterns of AKT RNA expression may change with tumor development, we examined AKT isoform expression by RT-PCR in human cancer cell lines, primary human cancers, and normal human tissues. Experimental Design: AKT-1, -2, and -3 RNA expression was examined by RT-PCR. Because up-regulated AKT-3 expression has been implicated in human breast and prostate cancer progression, we also examined AKT-3 expression levels by semiquantitative RT-PCR using matched normal/tumor first-strand cDNA pairs from colon, breast, prostate, and lung cancers. Results: Our data reveal that the overwhelming majority of both normal and tumor tissues express all three of the AKT isoforms. Moreover, semiquantitative RT-PCR of matched normal/tumor pairs confirmed similar AKT-3 RNA expression levels in both normal and tumor tissue. Conclusions: Our data show that both normal and tumor tissues express all three of the AKT isoforms and indicate that tumorigenesis does not involve a dramatic shift in the RNA expression patterns of the three AKT isoforms.

UR - http://www.scopus.com/inward/record.url?scp=0034881622&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034881622&partnerID=8YFLogxK

M3 - Article

VL - 7

SP - 2475

EP - 2479

JO - Clinical Cancer Research

JF - Clinical Cancer Research

SN - 1078-0432

IS - 8

ER -