Alcohol activates the hedgehog pathway and induces related procarcinogenic processes in the alcohol-preferring rat model of hepatocarcinogenesis

Isaac S. Chan, Cynthia D. Guy, Mariana V. Machado, Abigail Wank, Vishnu Kadiyala, Gregory Michelotti, Steve Choi, Marzena Swiderska-Syn, Gamze Karaca, Thiago A. Pereira, Michele T. Yip-Schneider, C. Max Schmidt, Anna Mae Diehl

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Background: Alcohol consumption promotes hepatocellular carcinoma (HCC). The responsible mechanisms are not well understood. Hepatocarcinogenesis increases with age and is enhanced by factors that impose a demand for liver regeneration. Because alcohol is hepatotoxic, habitual alcohol ingestion evokes a recurrent demand for hepatic regeneration. The alcohol-preferring (P) rat model mimics the level of alcohol consumption by humans who habitually abuse alcohol. Previously, we showed that habitual heavy alcohol ingestion amplified age-related hepatocarcinogenesis in P rats, with over 80% of alcohol-consuming P rats developing HCCs after 18 months of alcohol exposure, compared with only 5% of water-drinking controls. Methods: Herein, we used quantitative real-time PCR and quantitative immunocytochemistry to compare liver tissues from alcohol-consuming P rats and water-fed P rat controls after 6, 12, or 18 months of drinking. We aimed to identify potential mechanisms that might underlie the differences in liver cancer formation and hypothesized that chronic alcohol ingestion would activate Hedgehog (HH), a regenerative signaling pathway that is overactivated in HCC. Results: Chronic alcohol ingestion amplified age-related degenerative changes in hepatocytes, but did not cause appreciable liver inflammation or fibrosis even after 18 months of heavy drinking. HH signaling was also enhanced by alcohol exposure, as evidenced by increased levels of mRNAs encoding HH ligands, HH-regulated transcription factors, and HH target genes. Immunocytochemistry confirmed increased alcohol-related accumulation of HH ligand-producing cells and HH-responsive target cells. HH-related regenerative responses were also induced in alcohol-exposed rats. Three of these processes (i.e., deregulated progenitor expansion, the reverse Warburg effect, and epithelial-to-mesenchymal transitions) are known to promote cancer growth in other tissues. Conclusions: Alcohol-related changes in Hedgehog signaling and resultant deregulation of liver cell replacement might promote hepatocarcinogenesis.

Original languageEnglish (US)
Pages (from-to)787-800
Number of pages14
JournalAlcoholism: Clinical and Experimental Research
Volume38
Issue number3
DOIs
StatePublished - Mar 2014

Keywords

  • Epithelial-to-mesenchymal transitions
  • Hedgehog
  • Liver cancer
  • Liver progenitors
  • Reverse Warburg effect

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Psychiatry and Mental health
  • Toxicology

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    Chan, I. S., Guy, C. D., Machado, M. V., Wank, A., Kadiyala, V., Michelotti, G., Choi, S., Swiderska-Syn, M., Karaca, G., Pereira, T. A., Yip-Schneider, M. T., Max Schmidt, C., & Diehl, A. M. (2014). Alcohol activates the hedgehog pathway and induces related procarcinogenic processes in the alcohol-preferring rat model of hepatocarcinogenesis. Alcoholism: Clinical and Experimental Research, 38(3), 787-800. https://doi.org/10.1111/acer.12279