Alcohol enhances unprovoked 22-28 kHz USVs and suppresses USV mean frequency in High Alcohol Drinking (HAD-1) male rats

Neha Thakore, James M. Reno, Rueben A. Gonzales, Timothy Schallert, Richard Bell, W. Todd Maddox, Christine L. Duvauchelle

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Heightened emotional states increase impulsive behaviors such as excessive ethanol consumption in humans. Though positive and negative affective states in rodents can be monitored in real-time through ultrasonic vocalization (USV) emissions, few animal studies have focused on the role of emotional status as a stimulus for initial ethanol drinking. Our laboratory has recently developed reliable, high-speed analysis techniques to compile USV data during multiple-hour drinking sessions. Since High Alcohol Drinking (HAD-1) rats are selectively bred to voluntarily consume intoxicating levels of alcohol, we hypothesized that USVs emitted by HAD-1 rats would reveal unique emotional phenotypes predictive of alcohol intake and sensitive to alcohol experience. In this study, male HAD-1 rats had access to water, 15% and 30% EtOH or water only (i.e., Controls) during 8 weeks of daily 7-h drinking-in-the-dark (DID) sessions. USVs, associated with both positive (i.e., 50-55 kHz frequency-modulated or FM) and negative (i.e., 22-28 kHz) emotional states, emitted during these daily DID sessions were examined. Findings showed basal 22-28. kHz USVs were emitted by both EtOH-Naïve (Control) and EtOH-experienced rats, alcohol experience enhanced 22-28. kHz USV emissions, and USV acoustic parameters (i.e., mean frequency in kHz) of both positive and negative USVs were significantly suppressed by chronic alcohol experience. These data suggest that negative affective status initiates and maintains excessive alcohol intake in selectively bred HAD-1 rats and support the notion that unprovoked emissions of negative affect-associated USVs (i.e., 22-28 kHz) predict vulnerability to excessive alcohol intake in distinct rodent models.

Original languageEnglish (US)
Pages (from-to)228-236
Number of pages9
JournalBehavioural Brain Research
Volume302
DOIs
StatePublished - Apr 1 2016

Fingerprint

Ultrasonics
Alcohol Drinking
Alcohols
Drinking
Rodentia
Ethanol
Impulsive Behavior
Water
Acoustics
Phenotype

Keywords

  • Drinking-in-the-dark
  • Emotional status
  • Excessive alcohol intake
  • Negative affect
  • WAAVES

ASJC Scopus subject areas

  • Behavioral Neuroscience

Cite this

Alcohol enhances unprovoked 22-28 kHz USVs and suppresses USV mean frequency in High Alcohol Drinking (HAD-1) male rats. / Thakore, Neha; Reno, James M.; Gonzales, Rueben A.; Schallert, Timothy; Bell, Richard; Maddox, W. Todd; Duvauchelle, Christine L.

In: Behavioural Brain Research, Vol. 302, 01.04.2016, p. 228-236.

Research output: Contribution to journalArticle

Thakore, Neha ; Reno, James M. ; Gonzales, Rueben A. ; Schallert, Timothy ; Bell, Richard ; Maddox, W. Todd ; Duvauchelle, Christine L. / Alcohol enhances unprovoked 22-28 kHz USVs and suppresses USV mean frequency in High Alcohol Drinking (HAD-1) male rats. In: Behavioural Brain Research. 2016 ; Vol. 302. pp. 228-236.
@article{7772116b8baa4054920780a6611bafeb,
title = "Alcohol enhances unprovoked 22-28 kHz USVs and suppresses USV mean frequency in High Alcohol Drinking (HAD-1) male rats",
abstract = "Heightened emotional states increase impulsive behaviors such as excessive ethanol consumption in humans. Though positive and negative affective states in rodents can be monitored in real-time through ultrasonic vocalization (USV) emissions, few animal studies have focused on the role of emotional status as a stimulus for initial ethanol drinking. Our laboratory has recently developed reliable, high-speed analysis techniques to compile USV data during multiple-hour drinking sessions. Since High Alcohol Drinking (HAD-1) rats are selectively bred to voluntarily consume intoxicating levels of alcohol, we hypothesized that USVs emitted by HAD-1 rats would reveal unique emotional phenotypes predictive of alcohol intake and sensitive to alcohol experience. In this study, male HAD-1 rats had access to water, 15{\%} and 30{\%} EtOH or water only (i.e., Controls) during 8 weeks of daily 7-h drinking-in-the-dark (DID) sessions. USVs, associated with both positive (i.e., 50-55 kHz frequency-modulated or FM) and negative (i.e., 22-28 kHz) emotional states, emitted during these daily DID sessions were examined. Findings showed basal 22-28. kHz USVs were emitted by both EtOH-Na{\"i}ve (Control) and EtOH-experienced rats, alcohol experience enhanced 22-28. kHz USV emissions, and USV acoustic parameters (i.e., mean frequency in kHz) of both positive and negative USVs were significantly suppressed by chronic alcohol experience. These data suggest that negative affective status initiates and maintains excessive alcohol intake in selectively bred HAD-1 rats and support the notion that unprovoked emissions of negative affect-associated USVs (i.e., 22-28 kHz) predict vulnerability to excessive alcohol intake in distinct rodent models.",
keywords = "Drinking-in-the-dark, Emotional status, Excessive alcohol intake, Negative affect, WAAVES",
author = "Neha Thakore and Reno, {James M.} and Gonzales, {Rueben A.} and Timothy Schallert and Richard Bell and Maddox, {W. Todd} and Duvauchelle, {Christine L.}",
year = "2016",
month = "4",
day = "1",
doi = "10.1016/j.bbr.2016.01.042",
language = "English (US)",
volume = "302",
pages = "228--236",
journal = "Behavioural Brain Research",
issn = "0166-4328",
publisher = "Elsevier",

}

TY - JOUR

T1 - Alcohol enhances unprovoked 22-28 kHz USVs and suppresses USV mean frequency in High Alcohol Drinking (HAD-1) male rats

AU - Thakore, Neha

AU - Reno, James M.

AU - Gonzales, Rueben A.

AU - Schallert, Timothy

AU - Bell, Richard

AU - Maddox, W. Todd

AU - Duvauchelle, Christine L.

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Heightened emotional states increase impulsive behaviors such as excessive ethanol consumption in humans. Though positive and negative affective states in rodents can be monitored in real-time through ultrasonic vocalization (USV) emissions, few animal studies have focused on the role of emotional status as a stimulus for initial ethanol drinking. Our laboratory has recently developed reliable, high-speed analysis techniques to compile USV data during multiple-hour drinking sessions. Since High Alcohol Drinking (HAD-1) rats are selectively bred to voluntarily consume intoxicating levels of alcohol, we hypothesized that USVs emitted by HAD-1 rats would reveal unique emotional phenotypes predictive of alcohol intake and sensitive to alcohol experience. In this study, male HAD-1 rats had access to water, 15% and 30% EtOH or water only (i.e., Controls) during 8 weeks of daily 7-h drinking-in-the-dark (DID) sessions. USVs, associated with both positive (i.e., 50-55 kHz frequency-modulated or FM) and negative (i.e., 22-28 kHz) emotional states, emitted during these daily DID sessions were examined. Findings showed basal 22-28. kHz USVs were emitted by both EtOH-Naïve (Control) and EtOH-experienced rats, alcohol experience enhanced 22-28. kHz USV emissions, and USV acoustic parameters (i.e., mean frequency in kHz) of both positive and negative USVs were significantly suppressed by chronic alcohol experience. These data suggest that negative affective status initiates and maintains excessive alcohol intake in selectively bred HAD-1 rats and support the notion that unprovoked emissions of negative affect-associated USVs (i.e., 22-28 kHz) predict vulnerability to excessive alcohol intake in distinct rodent models.

AB - Heightened emotional states increase impulsive behaviors such as excessive ethanol consumption in humans. Though positive and negative affective states in rodents can be monitored in real-time through ultrasonic vocalization (USV) emissions, few animal studies have focused on the role of emotional status as a stimulus for initial ethanol drinking. Our laboratory has recently developed reliable, high-speed analysis techniques to compile USV data during multiple-hour drinking sessions. Since High Alcohol Drinking (HAD-1) rats are selectively bred to voluntarily consume intoxicating levels of alcohol, we hypothesized that USVs emitted by HAD-1 rats would reveal unique emotional phenotypes predictive of alcohol intake and sensitive to alcohol experience. In this study, male HAD-1 rats had access to water, 15% and 30% EtOH or water only (i.e., Controls) during 8 weeks of daily 7-h drinking-in-the-dark (DID) sessions. USVs, associated with both positive (i.e., 50-55 kHz frequency-modulated or FM) and negative (i.e., 22-28 kHz) emotional states, emitted during these daily DID sessions were examined. Findings showed basal 22-28. kHz USVs were emitted by both EtOH-Naïve (Control) and EtOH-experienced rats, alcohol experience enhanced 22-28. kHz USV emissions, and USV acoustic parameters (i.e., mean frequency in kHz) of both positive and negative USVs were significantly suppressed by chronic alcohol experience. These data suggest that negative affective status initiates and maintains excessive alcohol intake in selectively bred HAD-1 rats and support the notion that unprovoked emissions of negative affect-associated USVs (i.e., 22-28 kHz) predict vulnerability to excessive alcohol intake in distinct rodent models.

KW - Drinking-in-the-dark

KW - Emotional status

KW - Excessive alcohol intake

KW - Negative affect

KW - WAAVES

UR - http://www.scopus.com/inward/record.url?scp=84955260965&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84955260965&partnerID=8YFLogxK

U2 - 10.1016/j.bbr.2016.01.042

DO - 10.1016/j.bbr.2016.01.042

M3 - Article

VL - 302

SP - 228

EP - 236

JO - Behavioural Brain Research

JF - Behavioural Brain Research

SN - 0166-4328

ER -