Alcohol-induced inhibition of alveolar macrophage oxidant release in vivo and in vitro

V. B. Antony, S. W. Godbey, J. W. Hott, Sherry Queener

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Alcohol consumption is known to predispose the host to more frequent and severe bacterial infections, suggesting that alcohol compromises the normal immune function of the lung. The pulmonary alveolar macrophage is the resident host defense cell in the lung and forms the first line of defense against invading microorganisms. One of the mechanisms whereby alveolar macrophages kill bacteria is by releasing toxic oxygen radical species, such as superoxide anion and hydrogen peroxide. We hypothesized that chronic alcohol consumption caused alveolar macrophage dysfunction leading to inhibition of oxidant production when stimulated. Our data demonstrate that alveolar macrophages harvested from alcohol-treated rats release significantly lower quantity (p <0.05) of both superoxide anion and hydrogen peroxide when stimulated with several different types of stimuli including heat-killed Staphylococcus aureus, soluble immune complexes or phorbol myristate acetate. Pair-fed control rats who received isocaloric quantities of maltose dextrin in their diet to compensate for the alcohol were able to produce oxidants in equal quantities when stimulated, to rats who were fed a normal diet. Similar results were noted in vitro experiments when alveolar macrophages harvested from normal rats were incubated in vitro in alcohol- containing media and then stimulated with the aforementioned stimuli. Alveolar macrophages, which had been incubated in alcohol for 4 hr, showed significant decreases in their ability to produce superoxide anion. This defect was noticeable for a period up to 8 hr following removal of alveolar macrophages from the alcohol-containing media. The inability of alveolar macrophages to release oxidants may cause a significant break in their bacteriocidal capacity leading to the establishment of overt pulmonary parenchymal infection.

Original languageEnglish (US)
Pages (from-to)389-393
Number of pages5
JournalAlcoholism: Clinical and Experimental Research
Volume17
Issue number2
StatePublished - 1993

Fingerprint

Alveolar Macrophages
Oxidants
Alcohols
Superoxides
Rats
Nutrition
Hydrogen Peroxide
Alcohol Drinking
Lung
Rat control
Diet
Maltose
In Vitro Techniques
Inhibition (Psychology)
Poisons
Tetradecanoylphorbol Acetate
Aptitude
Antigen-Antibody Complex
Microorganisms
Bacterial Infections

Keywords

  • Alcohol
  • Alveolar Macrophage
  • Oxidants
  • Pulmonary Infection

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Alcohol-induced inhibition of alveolar macrophage oxidant release in vivo and in vitro. / Antony, V. B.; Godbey, S. W.; Hott, J. W.; Queener, Sherry.

In: Alcoholism: Clinical and Experimental Research, Vol. 17, No. 2, 1993, p. 389-393.

Research output: Contribution to journalArticle

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