Alcohol intake of P rats is regulated by muscarinic receptors in the pedunculopontine nucleus and VTA

S. N. Katner, W. J. McBride, L. Lumeng, T. K. Li, J. M. Murphy

Research output: Contribution to journalArticle

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Abstract

Experiments were conducted to determine whether muscarinic receptors within the pedunculopontine nucleus (PPN) and ventral tegmental area (VTA) are involved in regulating ethanol drinking behavior in the alcohol-preferring P line of rats. Female P rats were given limited access (2 h/day) to 10% (v/v) ethanol and 0.0125% (g/100 ml) saccharin solutions. Food was available ad libitum. Cholinergic agents were microinjected unilaterally into the PPN or VTA immediately prior to ethanol access. Intra-PPN carbachol (1-4 μg/0.5 μl), which can inhibit cholinergic neuronal activity within the PPN, decreased ethanol (70% decrease at the highest dose; p < 0.05) and saccharin (90% decrease at the highest dose; p < 0.05) intake in a dose-dependent manner within the first 30 min. Intra-PPN scopolamine (5-15 μg/0.5 μl), which can stimulate cholinergic neuronal activity within the PPN, decreased ethanol intake in a dose-dependent manner within the first 30 min (65% decrease at the highest dose; p < 0.05) without reducing saccharin intake. Intra-VTA methylscopolamine (1-10 μg/0.5 μl), a muscarinic antagonist, significantly (p < 0.05) reduced ethanol (60% decrease at the highest dose) and saccharin (50% decrease at the highest dose) intakes during the 2-h access period. Intra-VTA carbachol, a cholinergic agonist (1 and 2 μg/0.5 μl) decreased ethanol consumption in a dose-dependent manner within the first 60 min (50% decrease at the highest dose) without reducing saccharin intake. Overall, these results support an involvement of the cholinergic PPN-VTA system in regulating alcohol drinking and general consummatory behaviors of the P line of rats.

Original languageEnglish
Pages (from-to)497-504
Number of pages8
JournalPharmacology Biochemistry and Behavior
Volume58
Issue number2
DOIs
StatePublished - Oct 1997

Fingerprint

Ventral Tegmental Area
Muscarinic Receptors
Saccharin
Rats
Ethanol
Alcohols
Cholinergic Agents
Carbachol
Consummatory Behavior
Cholinergic Agonists
Drinking Behavior
Muscarinic Antagonists
Scopolamine Hydrobromide
Alcohol Drinking
Food

Keywords

  • Alcohol drinking
  • Alcohol-preferring rats
  • Carbachol
  • Microinjection
  • Pedunculopontine nucleus
  • Scopolamine
  • Ventral tegmental area

ASJC Scopus subject areas

  • Biochemistry
  • Behavioral Neuroscience
  • Pharmacology

Cite this

Alcohol intake of P rats is regulated by muscarinic receptors in the pedunculopontine nucleus and VTA. / Katner, S. N.; McBride, W. J.; Lumeng, L.; Li, T. K.; Murphy, J. M.

In: Pharmacology Biochemistry and Behavior, Vol. 58, No. 2, 10.1997, p. 497-504.

Research output: Contribution to journalArticle

Katner, S. N. ; McBride, W. J. ; Lumeng, L. ; Li, T. K. ; Murphy, J. M. / Alcohol intake of P rats is regulated by muscarinic receptors in the pedunculopontine nucleus and VTA. In: Pharmacology Biochemistry and Behavior. 1997 ; Vol. 58, No. 2. pp. 497-504.
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