Alcohol-naïve alcohol-preferring (P) rats exhibit higher local cerebral glucose utilization than alcohol-nonpreferring (NP) and wistar rats

D. G. Smith, J. E. Learn, W. J. McBride, L. Lumeng, T. K. Li, J. M. Murphy

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: The present study determined local cerebral glucose utilization (LCGU) rates in alcohol-naïve alcohol-preferring (P), alcohol nonpreferring (NP), and outbred Wistar rats to test the hypothesis that innate differences in functional neuronal activity are present in limbic regions as a result of selective breeding for high-alcohol drinking behavior. Methods: All procedures were conducted during the dark cycle. 2-[14C]deoxyglucose ([14C]2-DG; 125 μCi/kg) was injected intravenously and timed arterial blood samples were collected during the following 45 min and assayed for glucose and [14C]2-DG content. Rats were then decapitated, the brains removed and frozen to -70°C, and 20 μm coronal sections were prepared for quantitative autoradiographic analysis. Results: Rates of LCGU were determined in 55 regions and subregions, including limbic, cortical, and subcortical structures. LCGU rates were significantly (p < 0.01) higher in several limbic (e.g., ventral tegmental area, nucleus accumbens shell, olfactory tubercle, medial prefrontal cortex, and lateral hypothalamus), cortical (e.g., parietal, temporal, occipital, cingulate, piriform, and entorhinal), and subcortical (e.g., thalamus, habenula, preoptic area, and striatum) regions in P rats, compared with NP and Wistar rats, whereas rates in Wistar rats were higher in a few regions (e.g., CA1 and CA3 regions of the posterior hippocampus) than NP rats. Conclusions: The data suggest that selective breeding for high-alcohol drinking produces intrinsically higher functional neuronal activity in the central nervous system regions of the high-alcohol consuming P line compared with low-alcohol drinking NP or Wistar rats, although these differences may not generalize to other rat lines selectively bred for divergent alcohol drinking.

Original languageEnglish
Pages (from-to)1309-1316
Number of pages8
JournalAlcoholism: Clinical and Experimental Research
Volume25
Issue number9
StatePublished - 2001

Fingerprint

Alcohol Drinking
Wistar Rats
Rats
Alcohols
Glucose
Tegmentum Mesencephali
Lateral Hypothalamic Area
Habenula
Drinking Behavior
Ventral Tegmental Area
Preoptic Area
Deoxyglucose
Nucleus Accumbens
Prefrontal Cortex
Thalamus
Hippocampus
Central Nervous System
Brain
Neurology
Blood

Keywords

  • 2-Deoxyglucose
  • Alcohol Nonpreferring Rats
  • Alcohol-Preferring Rats
  • Local Cerebral Glucose Utilization

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Alcohol-naïve alcohol-preferring (P) rats exhibit higher local cerebral glucose utilization than alcohol-nonpreferring (NP) and wistar rats. / Smith, D. G.; Learn, J. E.; McBride, W. J.; Lumeng, L.; Li, T. K.; Murphy, J. M.

In: Alcoholism: Clinical and Experimental Research, Vol. 25, No. 9, 2001, p. 1309-1316.

Research output: Contribution to journalArticle

Smith, D. G. ; Learn, J. E. ; McBride, W. J. ; Lumeng, L. ; Li, T. K. ; Murphy, J. M. / Alcohol-naïve alcohol-preferring (P) rats exhibit higher local cerebral glucose utilization than alcohol-nonpreferring (NP) and wistar rats. In: Alcoholism: Clinical and Experimental Research. 2001 ; Vol. 25, No. 9. pp. 1309-1316.
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T1 - Alcohol-naïve alcohol-preferring (P) rats exhibit higher local cerebral glucose utilization than alcohol-nonpreferring (NP) and wistar rats

AU - Smith, D. G.

AU - Learn, J. E.

AU - McBride, W. J.

AU - Lumeng, L.

AU - Li, T. K.

AU - Murphy, J. M.

PY - 2001

Y1 - 2001

N2 - Background: The present study determined local cerebral glucose utilization (LCGU) rates in alcohol-naïve alcohol-preferring (P), alcohol nonpreferring (NP), and outbred Wistar rats to test the hypothesis that innate differences in functional neuronal activity are present in limbic regions as a result of selective breeding for high-alcohol drinking behavior. Methods: All procedures were conducted during the dark cycle. 2-[14C]deoxyglucose ([14C]2-DG; 125 μCi/kg) was injected intravenously and timed arterial blood samples were collected during the following 45 min and assayed for glucose and [14C]2-DG content. Rats were then decapitated, the brains removed and frozen to -70°C, and 20 μm coronal sections were prepared for quantitative autoradiographic analysis. Results: Rates of LCGU were determined in 55 regions and subregions, including limbic, cortical, and subcortical structures. LCGU rates were significantly (p < 0.01) higher in several limbic (e.g., ventral tegmental area, nucleus accumbens shell, olfactory tubercle, medial prefrontal cortex, and lateral hypothalamus), cortical (e.g., parietal, temporal, occipital, cingulate, piriform, and entorhinal), and subcortical (e.g., thalamus, habenula, preoptic area, and striatum) regions in P rats, compared with NP and Wistar rats, whereas rates in Wistar rats were higher in a few regions (e.g., CA1 and CA3 regions of the posterior hippocampus) than NP rats. Conclusions: The data suggest that selective breeding for high-alcohol drinking produces intrinsically higher functional neuronal activity in the central nervous system regions of the high-alcohol consuming P line compared with low-alcohol drinking NP or Wistar rats, although these differences may not generalize to other rat lines selectively bred for divergent alcohol drinking.

AB - Background: The present study determined local cerebral glucose utilization (LCGU) rates in alcohol-naïve alcohol-preferring (P), alcohol nonpreferring (NP), and outbred Wistar rats to test the hypothesis that innate differences in functional neuronal activity are present in limbic regions as a result of selective breeding for high-alcohol drinking behavior. Methods: All procedures were conducted during the dark cycle. 2-[14C]deoxyglucose ([14C]2-DG; 125 μCi/kg) was injected intravenously and timed arterial blood samples were collected during the following 45 min and assayed for glucose and [14C]2-DG content. Rats were then decapitated, the brains removed and frozen to -70°C, and 20 μm coronal sections were prepared for quantitative autoradiographic analysis. Results: Rates of LCGU were determined in 55 regions and subregions, including limbic, cortical, and subcortical structures. LCGU rates were significantly (p < 0.01) higher in several limbic (e.g., ventral tegmental area, nucleus accumbens shell, olfactory tubercle, medial prefrontal cortex, and lateral hypothalamus), cortical (e.g., parietal, temporal, occipital, cingulate, piriform, and entorhinal), and subcortical (e.g., thalamus, habenula, preoptic area, and striatum) regions in P rats, compared with NP and Wistar rats, whereas rates in Wistar rats were higher in a few regions (e.g., CA1 and CA3 regions of the posterior hippocampus) than NP rats. Conclusions: The data suggest that selective breeding for high-alcohol drinking produces intrinsically higher functional neuronal activity in the central nervous system regions of the high-alcohol consuming P line compared with low-alcohol drinking NP or Wistar rats, although these differences may not generalize to other rat lines selectively bred for divergent alcohol drinking.

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