Alcohol-naïve USVs distinguish male HAD-1 from LAD-1 rat strains

Nitish Mittal, Neha Thakore, James M. Reno, Richard Bell, W. Todd Maddox, Timothy Schallert, Christine L. Duvauchelle

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Ultrasonic vocalizations (USVs) are mediated through specific dopaminergic and cholinergic neural pathways and serve as real-time measures of positive and negative emotional status in rodents. Although most USV studies focus primarily on USV counts, each USV possesses a number of characteristics shown to reflect activity in the associated neurotransmitter system. In the present study, we recorded spontaneously emitted USVs from alcohol-naïve high alcohol drinking (HAD-1) and low alcohol drinking (LAD-1) rats. Using our recently developed WAAVES algorithm, we quantified four acoustic characteristics (mean frequency, duration, power, and bandwidth) from each 22–28 kHz and 50–55 kHz frequency-modulated (FM) USV. This rich USV representation allowed us to apply advanced statistical techniques to identify the USV acoustic characteristics that distinguished HAD-1 from LAD-1 rats. Linear mixed models (LMM) examined the predictability of each USV characteristic in isolation and linear discriminant analysis (LDA), and binomial logistic regression examined the predictability of linear combinations of the USV characteristics as a group. Results revealed significant differences in acoustic characteristics between HAD-1 and LAD-1 rats in both 22–28 kHz and 50–55 kHz FM USVs. In other words, these rats selectively bred for high- and low-alcohol consumption can be identified as HAD-1 or LAD-1 rats with high classification accuracy (approximately 92–100%) exclusively based on their emitted 22–28 kHz and 50–55 kHz FM USV acoustic characteristics. In addition, acoustic characteristics of 22–28 kHz and 50–55 kHz FM USVs emitted by alcohol-naïve HAD-1 and LAD-1 rats significantly correlate with their future alcohol consumption. Our current findings provide novel evidence that USV acoustic characteristics can be used to discriminate between alcohol-naïve HAD-1 and LAD-1 rats, and may serve as biomarkers in rodents with a predisposition for, or against, excessive alcohol intake.

Original languageEnglish (US)
Pages (from-to)9-17
Number of pages9
JournalAlcohol
Volume68
DOIs
StatePublished - May 1 2018

Fingerprint

Ultrasonics
Rats
acoustics
alcohol
Alcohols
Acoustics
alcohol consumption
Alcohol Drinking
discriminant analysis
social isolation
Rodentia
logistics
Neural Pathways
regression
Biomarkers
Discriminant Analysis
Discriminant analysis
Cholinergic Agents
evidence
Neurotransmitter Agents

Keywords

  • Alcoholism biomarker
  • Binomial logistic regression
  • Linear discriminant analysis
  • Linear mixed model
  • Selectively bred rats

ASJC Scopus subject areas

  • Health(social science)
  • Biochemistry
  • Toxicology
  • Neurology
  • Behavioral Neuroscience

Cite this

Mittal, N., Thakore, N., Reno, J. M., Bell, R., Maddox, W. T., Schallert, T., & Duvauchelle, C. L. (2018). Alcohol-naïve USVs distinguish male HAD-1 from LAD-1 rat strains. Alcohol, 68, 9-17. https://doi.org/10.1016/j.alcohol.2017.09.003

Alcohol-naïve USVs distinguish male HAD-1 from LAD-1 rat strains. / Mittal, Nitish; Thakore, Neha; Reno, James M.; Bell, Richard; Maddox, W. Todd; Schallert, Timothy; Duvauchelle, Christine L.

In: Alcohol, Vol. 68, 01.05.2018, p. 9-17.

Research output: Contribution to journalArticle

Mittal, N, Thakore, N, Reno, JM, Bell, R, Maddox, WT, Schallert, T & Duvauchelle, CL 2018, 'Alcohol-naïve USVs distinguish male HAD-1 from LAD-1 rat strains', Alcohol, vol. 68, pp. 9-17. https://doi.org/10.1016/j.alcohol.2017.09.003
Mittal N, Thakore N, Reno JM, Bell R, Maddox WT, Schallert T et al. Alcohol-naïve USVs distinguish male HAD-1 from LAD-1 rat strains. Alcohol. 2018 May 1;68:9-17. https://doi.org/10.1016/j.alcohol.2017.09.003
Mittal, Nitish ; Thakore, Neha ; Reno, James M. ; Bell, Richard ; Maddox, W. Todd ; Schallert, Timothy ; Duvauchelle, Christine L. / Alcohol-naïve USVs distinguish male HAD-1 from LAD-1 rat strains. In: Alcohol. 2018 ; Vol. 68. pp. 9-17.
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abstract = "Ultrasonic vocalizations (USVs) are mediated through specific dopaminergic and cholinergic neural pathways and serve as real-time measures of positive and negative emotional status in rodents. Although most USV studies focus primarily on USV counts, each USV possesses a number of characteristics shown to reflect activity in the associated neurotransmitter system. In the present study, we recorded spontaneously emitted USVs from alcohol-na{\"i}ve high alcohol drinking (HAD-1) and low alcohol drinking (LAD-1) rats. Using our recently developed WAAVES algorithm, we quantified four acoustic characteristics (mean frequency, duration, power, and bandwidth) from each 22–28 kHz and 50–55 kHz frequency-modulated (FM) USV. This rich USV representation allowed us to apply advanced statistical techniques to identify the USV acoustic characteristics that distinguished HAD-1 from LAD-1 rats. Linear mixed models (LMM) examined the predictability of each USV characteristic in isolation and linear discriminant analysis (LDA), and binomial logistic regression examined the predictability of linear combinations of the USV characteristics as a group. Results revealed significant differences in acoustic characteristics between HAD-1 and LAD-1 rats in both 22–28 kHz and 50–55 kHz FM USVs. In other words, these rats selectively bred for high- and low-alcohol consumption can be identified as HAD-1 or LAD-1 rats with high classification accuracy (approximately 92–100{\%}) exclusively based on their emitted 22–28 kHz and 50–55 kHz FM USV acoustic characteristics. In addition, acoustic characteristics of 22–28 kHz and 50–55 kHz FM USVs emitted by alcohol-na{\"i}ve HAD-1 and LAD-1 rats significantly correlate with their future alcohol consumption. Our current findings provide novel evidence that USV acoustic characteristics can be used to discriminate between alcohol-na{\"i}ve HAD-1 and LAD-1 rats, and may serve as biomarkers in rodents with a predisposition for, or against, excessive alcohol intake.",
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