Aldosterone production after short-term culture of rat adrenal capsule: Responsiveness to angiotensin II, potassium and acth

J. Howard Pratt, Deborah A. Turner, James A. McAteer

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

In vitro studies of aldosterone production have traditionally used freshly isolated adrenal glomerulosa tissue. In the present study we examined the effects of short-term culture of rat adrenal capsule on its secretory capacity by measuring both basal and stimulated aldosterone production. Capsules were maintained in culture for 24 h, and then responses to administered angiotensin II (1 × 10-7M), potassium (an increase of 2mM) and ACTH (1 × 10-8M) were determined during perifusion. Results were compared with responses by freshly isolated adrenal capsule. Although short-term culture reduced basal aldosterone production, responsiveness to administered stimuli was intact and often was greater than that observed with fresh capsular tissue. The results indicate that short-term culture of zona glomerulosa provides a suitable in vitro preparation for examining aldosterone secretory responsiveness to stimuli.

Original languageEnglish (US)
Pages (from-to)355-359
Number of pages5
JournalJournal of Steroid Biochemistry
Volume26
Issue number3
DOIs
StatePublished - Mar 1987

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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