Alignment and composition of laminin-polycaprolactone nanofiber blends enhance peripheral nerve regeneration

Rebekah A. Neal, Sunil S. Tholpady, Patricia L. Foley, Nathan Swami, Roy C. Ogle, Edward A. Botchwey

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Peripheral nerve transection occurs commonly in traumatic injury, causing deficits distal to the injury site. Conduits for repair currently on the market are hollow tubes; however, they often fail due to slow regeneration over long gaps. To facilitate increased regeneration speed and functional recovery, the ideal conduit should provide biochemically relevant signals and physical guidance cues, thus playing an active role in regeneration. To that end, laminin and laminin-polycaprolactone (PCL) blend nanofibers were fabricated to mimic peripheral nerve basement membrane. In vitro assays established 10% (wt) laminin content is sufficient to retain neurite-promoting effects of laminin. In addition, modified collector plate design to introduce an insulating gap enabled the fabrication of aligned nanofibers. The effects of laminin content and fiber orientation were evaluated in rat tibial nerve defect model. The lumens of conduits were filled with nanofiber meshes of varying laminin content and alignment to assess changes in motor and sensory recovery. Retrograde nerve conduction speed at 6 weeks was significantly faster in animals receiving aligned nanofiber conduits than in those receiving random nanofiber conduits. Animals receiving nanofiber-filled conduits showed some conduction in both anterograde and retrograde directions, whereas in animals receiving hollow conduits, no impulse conduction was detected. Aligned PCL nanofibers significantly improved motor function; aligned laminin blend nanofibers yielded the best sensory function recovery. In both cases, nanofiber-filled conduits resulted in better functional recovery than hollow conduits. These studies provide a firm foundation for the use of natural-synthetic blend electrospun nanofibers to enhance existing hollow nerve guidance conduits.

Original languageEnglish (US)
Pages (from-to)406-423
Number of pages18
JournalJournal of Biomedical Materials Research - Part A
Volume100 A
Issue number2
DOIs
StatePublished - Feb 2012
Externally publishedYes

Fingerprint

Nanofibers
Polycaprolactone
Nerve Regeneration
Laminin
Peripheral Nerves
Chemical analysis
Regeneration
Recovery
Animals
polycaprolactone
Tibial Nerve
Neural Conduction
Wounds and Injuries
Neurites
Fiber reinforced materials
Basement Membrane
Cues
Rats
Assays
Repair

Keywords

  • biomimetic material
  • ECM
  • laminin
  • nanotopography
  • nerve regeneration

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys
  • Medicine(all)

Cite this

Alignment and composition of laminin-polycaprolactone nanofiber blends enhance peripheral nerve regeneration. / Neal, Rebekah A.; Tholpady, Sunil S.; Foley, Patricia L.; Swami, Nathan; Ogle, Roy C.; Botchwey, Edward A.

In: Journal of Biomedical Materials Research - Part A, Vol. 100 A, No. 2, 02.2012, p. 406-423.

Research output: Contribution to journalArticle

Neal, Rebekah A. ; Tholpady, Sunil S. ; Foley, Patricia L. ; Swami, Nathan ; Ogle, Roy C. ; Botchwey, Edward A. / Alignment and composition of laminin-polycaprolactone nanofiber blends enhance peripheral nerve regeneration. In: Journal of Biomedical Materials Research - Part A. 2012 ; Vol. 100 A, No. 2. pp. 406-423.
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