Allelic analysis on chromosome 5 in sporadic colorectal cancer patients

Fang Zhang, Chongzhi Zhou, Yun Ling, Guoqiang Qiu, Shaochun Bai, Wanqing Liu, Lin He, Zhihai Peng

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

OBJECTIVE: To evaluate and map the putative tumor suppressor loci on chromosome 5 involved in tumor progress or metastasis. METHODS: Chromosome 5 of 83 patients with sporadic colorectal cancer was systemically screened. Fifteen microsatellite marker primers labeled with 3 different fluorescents were used to amplify the corresponding loci of the genome DNA. The PCR products were electrophoresed on a 377 PRISM sequencer and the fluorescent signals were analyzed with Genotyper and Genescan software. RESULTS: The highest loss of heterozygosity (LOH) ratio was found at D5S416 (48.15%) on 5p and at D5S471 (38.71%) on 5q. The region (5q13.3 - 31), where D5S471 and 3 neighboring loci (D5S428, D5S2027 and D5S2115) reside, presented high frequent LOH. CONCLUSION: The deletion of APC, MCC, CTNNA1 and IL cluster in the 5q 13.3 - 31.1 area play important role in the tumorogenesis of colorectal cancer, and the expected existence of another novel tumor suppressor gene on 5p is possible.

Original languageEnglish (US)
Pages (from-to)458-460
Number of pages3
JournalZhonghua zhong liu za zhi [Chinese journal of oncology]
Volume24
Issue number5
StatePublished - Sep 2002
Externally publishedYes

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Chromosomes, Human, Pair 5
Loss of Heterozygosity
Colorectal Neoplasms
Tumor Suppressor Genes
Microsatellite Repeats
Neoplasms
Software
Genome
Neoplasm Metastasis
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Zhang, F., Zhou, C., Ling, Y., Qiu, G., Bai, S., Liu, W., ... Peng, Z. (2002). Allelic analysis on chromosome 5 in sporadic colorectal cancer patients. Zhonghua zhong liu za zhi [Chinese journal of oncology], 24(5), 458-460.

Allelic analysis on chromosome 5 in sporadic colorectal cancer patients. / Zhang, Fang; Zhou, Chongzhi; Ling, Yun; Qiu, Guoqiang; Bai, Shaochun; Liu, Wanqing; He, Lin; Peng, Zhihai.

In: Zhonghua zhong liu za zhi [Chinese journal of oncology], Vol. 24, No. 5, 09.2002, p. 458-460.

Research output: Contribution to journalArticle

Zhang, F, Zhou, C, Ling, Y, Qiu, G, Bai, S, Liu, W, He, L & Peng, Z 2002, 'Allelic analysis on chromosome 5 in sporadic colorectal cancer patients', Zhonghua zhong liu za zhi [Chinese journal of oncology], vol. 24, no. 5, pp. 458-460.
Zhang, Fang ; Zhou, Chongzhi ; Ling, Yun ; Qiu, Guoqiang ; Bai, Shaochun ; Liu, Wanqing ; He, Lin ; Peng, Zhihai. / Allelic analysis on chromosome 5 in sporadic colorectal cancer patients. In: Zhonghua zhong liu za zhi [Chinese journal of oncology]. 2002 ; Vol. 24, No. 5. pp. 458-460.
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AU - Liu, Wanqing

AU - He, Lin

AU - Peng, Zhihai

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N2 - OBJECTIVE: To evaluate and map the putative tumor suppressor loci on chromosome 5 involved in tumor progress or metastasis. METHODS: Chromosome 5 of 83 patients with sporadic colorectal cancer was systemically screened. Fifteen microsatellite marker primers labeled with 3 different fluorescents were used to amplify the corresponding loci of the genome DNA. The PCR products were electrophoresed on a 377 PRISM sequencer and the fluorescent signals were analyzed with Genotyper and Genescan software. RESULTS: The highest loss of heterozygosity (LOH) ratio was found at D5S416 (48.15%) on 5p and at D5S471 (38.71%) on 5q. The region (5q13.3 - 31), where D5S471 and 3 neighboring loci (D5S428, D5S2027 and D5S2115) reside, presented high frequent LOH. CONCLUSION: The deletion of APC, MCC, CTNNA1 and IL cluster in the 5q 13.3 - 31.1 area play important role in the tumorogenesis of colorectal cancer, and the expected existence of another novel tumor suppressor gene on 5p is possible.

AB - OBJECTIVE: To evaluate and map the putative tumor suppressor loci on chromosome 5 involved in tumor progress or metastasis. METHODS: Chromosome 5 of 83 patients with sporadic colorectal cancer was systemically screened. Fifteen microsatellite marker primers labeled with 3 different fluorescents were used to amplify the corresponding loci of the genome DNA. The PCR products were electrophoresed on a 377 PRISM sequencer and the fluorescent signals were analyzed with Genotyper and Genescan software. RESULTS: The highest loss of heterozygosity (LOH) ratio was found at D5S416 (48.15%) on 5p and at D5S471 (38.71%) on 5q. The region (5q13.3 - 31), where D5S471 and 3 neighboring loci (D5S428, D5S2027 and D5S2115) reside, presented high frequent LOH. CONCLUSION: The deletion of APC, MCC, CTNNA1 and IL cluster in the 5q 13.3 - 31.1 area play important role in the tumorogenesis of colorectal cancer, and the expected existence of another novel tumor suppressor gene on 5p is possible.

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