Allelic analysis on chromosome 5 in sporadic colorectal cancer patients

Fang Zhang, Chongzhi Zhou, Yun Ling, Guoqiang Qiu, Shaochun Bai, Wanqing Liu, Lin He, Zhihai Peng

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

OBJECTIVE: To evaluate and map the putative tumor suppressor loci on chromosome 5 involved in tumor progress or metastasis. METHODS: Chromosome 5 of 83 patients with sporadic colorectal cancer was systemically screened. Fifteen microsatellite marker primers labeled with 3 different fluorescents were used to amplify the corresponding loci of the genome DNA. The PCR products were electrophoresed on a 377 PRISM sequencer and the fluorescent signals were analyzed with Genotyper and Genescan software. RESULTS: The highest loss of heterozygosity (LOH) ratio was found at D5S416 (48.15%) on 5p and at D5S471 (38.71%) on 5q. The region (5q13.3 - 31), where D5S471 and 3 neighboring loci (D5S428, D5S2027 and D5S2115) reside, presented high frequent LOH. CONCLUSION: The deletion of APC, MCC, CTNNA1 and IL cluster in the 5q 13.3 - 31.1 area play important role in the tumorogenesis of colorectal cancer, and the expected existence of another novel tumor suppressor gene on 5p is possible.

Original languageEnglish (US)
Pages (from-to)458-460
Number of pages3
JournalZhonghua zhong liu za zhi [Chinese journal of oncology]
Volume24
Issue number5
StatePublished - Sep 2002

ASJC Scopus subject areas

  • Medicine(all)

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    Zhang, F., Zhou, C., Ling, Y., Qiu, G., Bai, S., Liu, W., He, L., & Peng, Z. (2002). Allelic analysis on chromosome 5 in sporadic colorectal cancer patients. Zhonghua zhong liu za zhi [Chinese journal of oncology], 24(5), 458-460.