Allelic imbalance of the mutant and wild-type RET allele in MEN 2A-associated medullary thyroid carcinoma

Christian A. Koch, Steve C. Huang, Jeffrey F. Moley, Norio Azumi, George P. Chrousos, Robert F. Gagel, Zhengping Zhuang, Karel Pacak, Alexander Vortmeyer

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Germline mutations of the RET proto-oncogene are responsible for the familial tumor syndrome called multiple endocrine neoplasia type 2 (MEN 2) that includes medullary thyroid carcinoma (MTC). Although inherited mutations of RET lead to tumor formation in patients with MEN 2, it is not understood why only selected cells develop into tumors. We have recently shown that duplication of the mutated RET allele or loss of the wild-type allele might represent mechanisms of tumorigenesis in patients with MEN 2A-related pheochromocytoma. We now analysed 19 DNA samples of MTC (15 of which were non-microdissected, four of which were microdissected) from patients with MEN 2A. Using polymorphic marker and phosphorimage densitometry analyses, we found allelic imbalance of the mutated and wild-type RET allele in six of 19 DNA MTC samples. Of note, two of the four microdissected tumor DNA samples showed allelic imbalance of RET, whereas only four of the 15 non-microdissected MTC samples did. These results underscore the significance of microdissection in the analysis of tumor DNA. In our study, some of the non-microdissected tumor DNA samples may have failed to display allelic imbalance of RET, because of contamination of tumor DNA with nonneoplastic DNA or noninformative microsatellite marker analysis. Taken together, our results suggest allelic imbalance between mutated and wild-type RET as a possible mechanism for tumor formation in some patients with MEN 2A-related MTC.

Original languageEnglish (US)
Pages (from-to)7809-7811
Number of pages3
JournalOncogene
Volume20
Issue number53
DOIs
StatePublished - Nov 22 2001
Externally publishedYes

Fingerprint

Allelic Imbalance
Multiple Endocrine Neoplasia Type 2a
Alleles
DNA
Neoplasms
DNA Contamination
Microdissection
Proto-Oncogenes
Medullary Thyroid cancer
Germ-Line Mutation
Densitometry
Pheochromocytoma
Microsatellite Repeats
Carcinogenesis
Mutation

Keywords

  • Allelic imbalance
  • Medullary thyroid carcinoma
  • MEN 2
  • RET

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Allelic imbalance of the mutant and wild-type RET allele in MEN 2A-associated medullary thyroid carcinoma. / Koch, Christian A.; Huang, Steve C.; Moley, Jeffrey F.; Azumi, Norio; Chrousos, George P.; Gagel, Robert F.; Zhuang, Zhengping; Pacak, Karel; Vortmeyer, Alexander.

In: Oncogene, Vol. 20, No. 53, 22.11.2001, p. 7809-7811.

Research output: Contribution to journalArticle

Koch, CA, Huang, SC, Moley, JF, Azumi, N, Chrousos, GP, Gagel, RF, Zhuang, Z, Pacak, K & Vortmeyer, A 2001, 'Allelic imbalance of the mutant and wild-type RET allele in MEN 2A-associated medullary thyroid carcinoma', Oncogene, vol. 20, no. 53, pp. 7809-7811. https://doi.org/10.1038/sj.onc.1204991
Koch CA, Huang SC, Moley JF, Azumi N, Chrousos GP, Gagel RF et al. Allelic imbalance of the mutant and wild-type RET allele in MEN 2A-associated medullary thyroid carcinoma. Oncogene. 2001 Nov 22;20(53):7809-7811. https://doi.org/10.1038/sj.onc.1204991
Koch, Christian A. ; Huang, Steve C. ; Moley, Jeffrey F. ; Azumi, Norio ; Chrousos, George P. ; Gagel, Robert F. ; Zhuang, Zhengping ; Pacak, Karel ; Vortmeyer, Alexander. / Allelic imbalance of the mutant and wild-type RET allele in MEN 2A-associated medullary thyroid carcinoma. In: Oncogene. 2001 ; Vol. 20, No. 53. pp. 7809-7811.
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