Alloantigen priming induces a state of unresponsiveness in human umbilical cord blood T cells

Grant Risdon, Jay Gaddy, Masato Horie, Hal Broxmeyer

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Induction of alloreactivity in human adult and umbilical cord blood T cells was evaluated in mixed leukocyte culture by exposure to an allogeneic lymphoblastoid line that expresses known costimulatory molecules. Initial exposure to alloantigen-presenting cells (allo-APC) induced strong proliferative responses in both adult and cord blood T cells. However, in contrast to adult T cells, cord blood T cells exhibited little proliferation after restimulation with donor APC. Primed cord blood T cells could respond to interleukin 2 (IL-2), but unresponsiveness to alloantigen was not overcome by addition of exogenous IL-2. Unresponsiveness was long-lasting and appeared to be maintained by a combination of induction of anergy and activity of CD8+ suppressor cells. This information may contribute to use of human cord blood as an allogeneic source of transplantable stem cells.

Original languageEnglish
Pages (from-to)2413-2417
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number6
DOIs
StatePublished - Mar 14 1995

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Isoantigens
Fetal Blood
Blood Cells
T-Lymphocytes
Interleukin-2
Leukocytes
Stem Cells

Keywords

  • T lymphocytes
  • tolerance

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Alloantigen priming induces a state of unresponsiveness in human umbilical cord blood T cells. / Risdon, Grant; Gaddy, Jay; Horie, Masato; Broxmeyer, Hal.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 6, 14.03.1995, p. 2413-2417.

Research output: Contribution to journalArticle

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