Alloantigen priming induces a state of unresponsiveness in human umbilical cord blood T cells

Grant Risdon, Jay Gaddy, Masato Horie, Hal E. Broxmeyer

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

Induction of alloreactivity in human adult and umbilical cord blood T cells was evaluated in mixed leukocyte culture by exposure to an allogeneic lymphoblastoid line that expresses known costimulatory molecules. Initial exposure to alloantigen-presenting cells (allo-APC) induced strong proliferative responses in both adult and cord blood T cells. However, in contrast to adult T cells, cord blood T cells exhibited little proliferation after restimulation with donor APC. Primed cord blood T cells could respond to interleukin 2 (IL-2), but unresponsiveness to alloantigen was not overcome by addition of exogenous IL-2. Unresponsiveness was long-lasting and appeared to be maintained by a combination of induction of anergy and activity of CD8+ suppressor cells. This information may contribute to use of human cord blood as an allogeneic source of transplantable stem cells.

Original languageEnglish (US)
Pages (from-to)2413-2417
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number6
DOIs
StatePublished - Mar 14 1995

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Keywords

  • T lymphocytes
  • tolerance

ASJC Scopus subject areas

  • Genetics
  • General

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