Allogeneic responses of human umbilical cord blood

G. Risdon, J. Gaddy, Hal Broxmeyer

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Human umbilical cord blood (CB) is increasingly used as an alternative to bone marrow as a source of stem and progenitor cells for pediatric patients. We have evaluated the alloreactive potential of cord blood T cells in vitro. Our findings demonstrate that in a primary mixed leukocyte culture CB T cells demonstrate strong proliferative responses to allogeneic stimulation but little to no generation of cytotoxic effector function. Furthermore, restimulation of primary cultures results in a state of proliferative unresponsiveness. Such diminished cytotoxic and proliferative responses may, in part, be related to the low incidence of graft vs. host disease thus far noted in human CB transplants.

Original languageEnglish
Pages (from-to)566-572
Number of pages7
JournalBlood Cells
Volume20
Issue number2-3
StatePublished - 1994

Fingerprint

Fetal Blood
Blood Cells
Stem Cells
T-Lymphocytes
Transplants
Leukocytes
Bone Marrow
Pediatrics
Incidence

Keywords

  • allogeneic response
  • graft vs. host disease
  • human umbilical cord blood
  • mononuclear cells

ASJC Scopus subject areas

  • Hematology

Cite this

Risdon, G., Gaddy, J., & Broxmeyer, H. (1994). Allogeneic responses of human umbilical cord blood. Blood Cells, 20(2-3), 566-572.

Allogeneic responses of human umbilical cord blood. / Risdon, G.; Gaddy, J.; Broxmeyer, Hal.

In: Blood Cells, Vol. 20, No. 2-3, 1994, p. 566-572.

Research output: Contribution to journalArticle

Risdon, G, Gaddy, J & Broxmeyer, H 1994, 'Allogeneic responses of human umbilical cord blood', Blood Cells, vol. 20, no. 2-3, pp. 566-572.
Risdon, G. ; Gaddy, J. ; Broxmeyer, Hal. / Allogeneic responses of human umbilical cord blood. In: Blood Cells. 1994 ; Vol. 20, No. 2-3. pp. 566-572.
@article{86f60c1e8e4b425ebc62452147130d5a,
title = "Allogeneic responses of human umbilical cord blood",
abstract = "Human umbilical cord blood (CB) is increasingly used as an alternative to bone marrow as a source of stem and progenitor cells for pediatric patients. We have evaluated the alloreactive potential of cord blood T cells in vitro. Our findings demonstrate that in a primary mixed leukocyte culture CB T cells demonstrate strong proliferative responses to allogeneic stimulation but little to no generation of cytotoxic effector function. Furthermore, restimulation of primary cultures results in a state of proliferative unresponsiveness. Such diminished cytotoxic and proliferative responses may, in part, be related to the low incidence of graft vs. host disease thus far noted in human CB transplants.",
keywords = "allogeneic response, graft vs. host disease, human umbilical cord blood, mononuclear cells",
author = "G. Risdon and J. Gaddy and Hal Broxmeyer",
year = "1994",
language = "English",
volume = "20",
pages = "566--572",
journal = "Blood Cells, Molecules, and Diseases",
issn = "1079-9796",
publisher = "Academic Press Inc.",
number = "2-3",

}

TY - JOUR

T1 - Allogeneic responses of human umbilical cord blood

AU - Risdon, G.

AU - Gaddy, J.

AU - Broxmeyer, Hal

PY - 1994

Y1 - 1994

N2 - Human umbilical cord blood (CB) is increasingly used as an alternative to bone marrow as a source of stem and progenitor cells for pediatric patients. We have evaluated the alloreactive potential of cord blood T cells in vitro. Our findings demonstrate that in a primary mixed leukocyte culture CB T cells demonstrate strong proliferative responses to allogeneic stimulation but little to no generation of cytotoxic effector function. Furthermore, restimulation of primary cultures results in a state of proliferative unresponsiveness. Such diminished cytotoxic and proliferative responses may, in part, be related to the low incidence of graft vs. host disease thus far noted in human CB transplants.

AB - Human umbilical cord blood (CB) is increasingly used as an alternative to bone marrow as a source of stem and progenitor cells for pediatric patients. We have evaluated the alloreactive potential of cord blood T cells in vitro. Our findings demonstrate that in a primary mixed leukocyte culture CB T cells demonstrate strong proliferative responses to allogeneic stimulation but little to no generation of cytotoxic effector function. Furthermore, restimulation of primary cultures results in a state of proliferative unresponsiveness. Such diminished cytotoxic and proliferative responses may, in part, be related to the low incidence of graft vs. host disease thus far noted in human CB transplants.

KW - allogeneic response

KW - graft vs. host disease

KW - human umbilical cord blood

KW - mononuclear cells

UR - http://www.scopus.com/inward/record.url?scp=0028567860&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028567860&partnerID=8YFLogxK

M3 - Article

VL - 20

SP - 566

EP - 572

JO - Blood Cells, Molecules, and Diseases

JF - Blood Cells, Molecules, and Diseases

SN - 1079-9796

IS - 2-3

ER -