Alteration in the expression of signaling parameters following carbon ion irradiation

Anirban Mitra, Nagesh Bhat, Asitikanta Sarma, Malini Krishna

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Ionizing radiation induces DNA damage, which generates a complex array of genotoxic responses. These responses depend on the type of DNA damage, which in turn can lead to unique cellular responses. High LET radiation results in clustered damages. This evokes specific signaling responses, which can be cytotoxic or cytoprotective in nature. In the present study the effect of carbon ion irradiation on p44/42 MAPK and NF-κB, which are essentially survival factors, have been studied. Moreover, the effect of inhibition of DNA-PK, which is an important component of DNA repair mechanism, with wortmanin on these signaling factors has been studied. The expression of p44/42 MAPK was different at 0.1 Gy and 1 Gy and wortmanin was found to inhibit its expression. NF-κB expression was higher at 1 Gy than at 0.1 Gy and its expression is unaffected by inhibition of DNA-PK. The notable findings of this study are that the responses to high and low dose of high LET radiation are essentially different and the 6 h time point post irradiation is crucial in deciding the response and needs further investigation.

Original languageEnglish (US)
Pages (from-to)169-173
Number of pages5
JournalMolecular and Cellular Biochemistry
Volume276
Issue number1-2
DOIs
StatePublished - Aug 2005
Externally publishedYes

Fingerprint

Linear Energy Transfer
Mitogen-Activated Protein Kinase 3
Ion bombardment
DNA Damage
Carbon
Ions
Radiation
DNA
Ionizing Radiation
DNA Repair
Ionizing radiation
Dosimetry
Repair
Irradiation

Keywords

  • High LET radiation
  • NF-κB
  • p44/42MAPK
  • Signaling
  • Wortmanin

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Alteration in the expression of signaling parameters following carbon ion irradiation. / Mitra, Anirban; Bhat, Nagesh; Sarma, Asitikanta; Krishna, Malini.

In: Molecular and Cellular Biochemistry, Vol. 276, No. 1-2, 08.2005, p. 169-173.

Research output: Contribution to journalArticle

Mitra, Anirban ; Bhat, Nagesh ; Sarma, Asitikanta ; Krishna, Malini. / Alteration in the expression of signaling parameters following carbon ion irradiation. In: Molecular and Cellular Biochemistry. 2005 ; Vol. 276, No. 1-2. pp. 169-173.
@article{b9a6a169accd4cebae152a7b54fdb7d9,
title = "Alteration in the expression of signaling parameters following carbon ion irradiation",
abstract = "Ionizing radiation induces DNA damage, which generates a complex array of genotoxic responses. These responses depend on the type of DNA damage, which in turn can lead to unique cellular responses. High LET radiation results in clustered damages. This evokes specific signaling responses, which can be cytotoxic or cytoprotective in nature. In the present study the effect of carbon ion irradiation on p44/42 MAPK and NF-κB, which are essentially survival factors, have been studied. Moreover, the effect of inhibition of DNA-PK, which is an important component of DNA repair mechanism, with wortmanin on these signaling factors has been studied. The expression of p44/42 MAPK was different at 0.1 Gy and 1 Gy and wortmanin was found to inhibit its expression. NF-κB expression was higher at 1 Gy than at 0.1 Gy and its expression is unaffected by inhibition of DNA-PK. The notable findings of this study are that the responses to high and low dose of high LET radiation are essentially different and the 6 h time point post irradiation is crucial in deciding the response and needs further investigation.",
keywords = "High LET radiation, NF-κB, p44/42MAPK, Signaling, Wortmanin",
author = "Anirban Mitra and Nagesh Bhat and Asitikanta Sarma and Malini Krishna",
year = "2005",
month = "8",
doi = "10.1007/s11010-005-3903-5",
language = "English (US)",
volume = "276",
pages = "169--173",
journal = "Molecular and Cellular Biochemistry",
issn = "0300-8177",
publisher = "Springer Netherlands",
number = "1-2",

}

TY - JOUR

T1 - Alteration in the expression of signaling parameters following carbon ion irradiation

AU - Mitra, Anirban

AU - Bhat, Nagesh

AU - Sarma, Asitikanta

AU - Krishna, Malini

PY - 2005/8

Y1 - 2005/8

N2 - Ionizing radiation induces DNA damage, which generates a complex array of genotoxic responses. These responses depend on the type of DNA damage, which in turn can lead to unique cellular responses. High LET radiation results in clustered damages. This evokes specific signaling responses, which can be cytotoxic or cytoprotective in nature. In the present study the effect of carbon ion irradiation on p44/42 MAPK and NF-κB, which are essentially survival factors, have been studied. Moreover, the effect of inhibition of DNA-PK, which is an important component of DNA repair mechanism, with wortmanin on these signaling factors has been studied. The expression of p44/42 MAPK was different at 0.1 Gy and 1 Gy and wortmanin was found to inhibit its expression. NF-κB expression was higher at 1 Gy than at 0.1 Gy and its expression is unaffected by inhibition of DNA-PK. The notable findings of this study are that the responses to high and low dose of high LET radiation are essentially different and the 6 h time point post irradiation is crucial in deciding the response and needs further investigation.

AB - Ionizing radiation induces DNA damage, which generates a complex array of genotoxic responses. These responses depend on the type of DNA damage, which in turn can lead to unique cellular responses. High LET radiation results in clustered damages. This evokes specific signaling responses, which can be cytotoxic or cytoprotective in nature. In the present study the effect of carbon ion irradiation on p44/42 MAPK and NF-κB, which are essentially survival factors, have been studied. Moreover, the effect of inhibition of DNA-PK, which is an important component of DNA repair mechanism, with wortmanin on these signaling factors has been studied. The expression of p44/42 MAPK was different at 0.1 Gy and 1 Gy and wortmanin was found to inhibit its expression. NF-κB expression was higher at 1 Gy than at 0.1 Gy and its expression is unaffected by inhibition of DNA-PK. The notable findings of this study are that the responses to high and low dose of high LET radiation are essentially different and the 6 h time point post irradiation is crucial in deciding the response and needs further investigation.

KW - High LET radiation

KW - NF-κB

KW - p44/42MAPK

KW - Signaling

KW - Wortmanin

UR - http://www.scopus.com/inward/record.url?scp=24144463772&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24144463772&partnerID=8YFLogxK

U2 - 10.1007/s11010-005-3903-5

DO - 10.1007/s11010-005-3903-5

M3 - Article

VL - 276

SP - 169

EP - 173

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

SN - 0300-8177

IS - 1-2

ER -