Alterations in expression of myosin and myosin light chain kinases in response to vascular injury

Patricia J. Gallagher, Yijun Jin, Gayla Killough, Emily K. Blue, Volkhard Lindner

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Histochemical analysis of balloon-injured rat carotid arteries revealed a coordinated expression of nonmuscle myosin heavy chain-A and -B (NM-A and NM-B) in response to injury. Expression of these nonmuscle myosin forms shifts from the media to the adventitia and intima. In contrast, expression of smooth muscle myosin heavy chain-1 (SM-1) within the media is not altered, whereas smooth muscle myosin heavy chain-2 (SM-2) expression declines. Western blotting shows a statistically significant increase in expression of NM-A that occurs within 6 h in response to carotid injury, suggesting this myosin form may be an appropriate experimental marker for proliferating, migrating cells in injured vessels. No overall change in the relative expression level of NM-B was detected, suggesting that compensatory declines in media expression are balanced by increases in the intima and adventitia. Expression of SM-1 did not change in response to injury, whereas the expression of SM-2 significantly declined between 24 h and 7 days. Expression of myosin light chain kinase is also negatively regulated, and the decline in its expression parallels downregulation of SM-2.

Original languageEnglish (US)
Pages (from-to)C1078-C1087
JournalAmerican Journal of Physiology - Cell Physiology
Volume279
Issue number4 48-4
DOIs
StatePublished - 2000

Keywords

  • Restenosis
  • Smooth muscle
  • Vascular remodeling

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

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