Alterations in glial fibrillary acidic protein (GFAP) mRNA levels in the hamster facial motor nucleus: Effects of axotomy and testosterone

Kathryn Jones, Nancy B. Kinderman, Monica M. Oblinger

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Testosterone propionate (TP) administered at the time of facial nerve injury in the hamster accelerates the rate of regeneration. In this study, we tested the hypothesis that the mechanism by which TP augments peripheral nerve regeneration involves regulation of glial fibrillary acidic protein (GFAP) mRNA in the facial motor nucleus. Castrated male hamsters were subjected to right facial nerve transection, with half the animals implanted subcutaneously with Silastic capsules containing exogenous TP and the remainder sham implanted. Postoperative survival times were 0.25, 1, 2, 4, 7, and 14 d. Qualitative/quantitative analyses of both film and emulsion autoradiograms were accomplished. Axotomy, with or without TP, resulted in a dramatic increase in GFAP mRNA levels by 1 d postoperative on the axotomized side, relative to controls. GFAP mRNA levels remained elevated throughout all postoperative times in both the nonhormone- and TP-treated animals. Qualitative examination of the film autoradiograms indicated a generalized decrease in the amount of GFAP mRNA in the control and axotomized nuclei of TP-treated animals when compared to the control and axotomized nuclei, respectively, of nonhormone-treated animals. Statistical comparison of the values obtained for both the film and emulsion autoradiograms confirmed this impression. Thus, while the injury-induced increases in GFAP mRNA expression were not blocked by TP, the overall extent of the increase was significantly tempered by steroid treatment. These data suggest that hormonal modulation of the astrocytic response to peripheral nerve injury may be a contributing factor in the ability of steroids to enhance the regenerative capacities of injured motor neurons.

Original languageEnglish (US)
Pages (from-to)1359-1366
Number of pages8
JournalNeurochemical Research
Volume22
Issue number11
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

Testosterone Propionate
Axotomy
Glial Fibrillary Acidic Protein
Cricetinae
Testosterone
Messenger RNA
Animals
Emulsions
Facial Nerve Injuries
Steroids
Peripheral Nerve Injuries
Nerve Regeneration
Facial Nerve
Motor Neurons
Facial Nucleus
Peripheral Nerves
Neurons
Capsules
Regeneration
Modulation

Keywords

  • Androgens
  • Astrocytes
  • Axonal injury
  • GFAP
  • Gonadal steroids
  • Peripheral nervo regeneration
  • Reactive gliesis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Alterations in glial fibrillary acidic protein (GFAP) mRNA levels in the hamster facial motor nucleus : Effects of axotomy and testosterone. / Jones, Kathryn; Kinderman, Nancy B.; Oblinger, Monica M.

In: Neurochemical Research, Vol. 22, No. 11, 1997, p. 1359-1366.

Research output: Contribution to journalArticle

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