Alterations of amino acid transmitter systems in spinal cords of chronic paraplegic dogs

W. J. McBride, P. V. Hall, E. Chernet, J. T. Patrick, Scott Shapiro

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The high-affinity uptake of [3H]serotonin, [3H]glutamate, and [3H]γ-aminobutyric acid ([3H]GABA) and the Na+-independent binding of [3H]glutamate and [3H]GABA were studied using spinal cord preparations obtained from normal mongrel dogs and from dogs made paraplegia by midthoracic spinal cord crush. Lumbosacral regions of the spinal cord were removed either before (1 week) or after (3 to 8 weeks) onset of spasticity. A myelin-free synaptosomal fraction was obtained by centrifugation and used for studying high-affinity uptake and for preparing synaptic plasma membranes for Na+-independent binding experiments. For the paraplegic groups, the uptake of 30 nM [3H]serotonin was 66 and 18% of control values after 1 and 3 weeks, respectively. Eadie-Hofstee analysis of [3H]serotonin uptake showed a 90% reduction in V(max) for the paraplegic group relative to control values, thereby indicating the expected loss of descending serotonergic pathways. The high-affinity uptakes of 1 μM [3H]glutamate and [3H]GABA were the same in both the control and nonspastic paraplegic groups after 1 week. However, after 3 weeks, the uptakes of [3H]glutamate and [3H]GABA were 60-70% higher for the spastic group than for the control animals. For both amino acids, Eadie-Hofstee plots revealed no difference in K(m) and higher V(max) for the spastic group relative to control values. After 1 and 3 weeks, the Na+-independent binding of 5 nM [3H]glutamate was 40-85% higher and the binding of 10 nM [3H]GABA was 40-60% lower for the paraplegic groups relative to the values for the control animals. Scatchard analysis revealed significant changes in B(max) values for both amino acids. Overall, the data indicate an increase in segmental amino acid excitatory influence which occurred when signs of spasticity were evident.

Original languageEnglish
Pages (from-to)1625-1631
Number of pages7
JournalJournal of Neurochemistry
Volume42
Issue number6
StatePublished - 1984

Fingerprint

Glutamic Acid
Transmitters
Spinal Cord
gamma-Aminobutyric Acid
Dogs
Amino Acids
Serotonin
Muscle Spasticity
Animals
Aminobutyrates
Lumbosacral Region
Synaptic Membranes
Excitatory Amino Acids
Paraplegia
Myelin Sheath
Centrifugation
Cell membranes
Cell Membrane
Control Groups
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

McBride, W. J., Hall, P. V., Chernet, E., Patrick, J. T., & Shapiro, S. (1984). Alterations of amino acid transmitter systems in spinal cords of chronic paraplegic dogs. Journal of Neurochemistry, 42(6), 1625-1631.

Alterations of amino acid transmitter systems in spinal cords of chronic paraplegic dogs. / McBride, W. J.; Hall, P. V.; Chernet, E.; Patrick, J. T.; Shapiro, Scott.

In: Journal of Neurochemistry, Vol. 42, No. 6, 1984, p. 1625-1631.

Research output: Contribution to journalArticle

McBride, WJ, Hall, PV, Chernet, E, Patrick, JT & Shapiro, S 1984, 'Alterations of amino acid transmitter systems in spinal cords of chronic paraplegic dogs', Journal of Neurochemistry, vol. 42, no. 6, pp. 1625-1631.
McBride, W. J. ; Hall, P. V. ; Chernet, E. ; Patrick, J. T. ; Shapiro, Scott. / Alterations of amino acid transmitter systems in spinal cords of chronic paraplegic dogs. In: Journal of Neurochemistry. 1984 ; Vol. 42, No. 6. pp. 1625-1631.
@article{516f53d93fb640b986856164990b55ed,
title = "Alterations of amino acid transmitter systems in spinal cords of chronic paraplegic dogs",
abstract = "The high-affinity uptake of [3H]serotonin, [3H]glutamate, and [3H]γ-aminobutyric acid ([3H]GABA) and the Na+-independent binding of [3H]glutamate and [3H]GABA were studied using spinal cord preparations obtained from normal mongrel dogs and from dogs made paraplegia by midthoracic spinal cord crush. Lumbosacral regions of the spinal cord were removed either before (1 week) or after (3 to 8 weeks) onset of spasticity. A myelin-free synaptosomal fraction was obtained by centrifugation and used for studying high-affinity uptake and for preparing synaptic plasma membranes for Na+-independent binding experiments. For the paraplegic groups, the uptake of 30 nM [3H]serotonin was 66 and 18{\%} of control values after 1 and 3 weeks, respectively. Eadie-Hofstee analysis of [3H]serotonin uptake showed a 90{\%} reduction in V(max) for the paraplegic group relative to control values, thereby indicating the expected loss of descending serotonergic pathways. The high-affinity uptakes of 1 μM [3H]glutamate and [3H]GABA were the same in both the control and nonspastic paraplegic groups after 1 week. However, after 3 weeks, the uptakes of [3H]glutamate and [3H]GABA were 60-70{\%} higher for the spastic group than for the control animals. For both amino acids, Eadie-Hofstee plots revealed no difference in K(m) and higher V(max) for the spastic group relative to control values. After 1 and 3 weeks, the Na+-independent binding of 5 nM [3H]glutamate was 40-85{\%} higher and the binding of 10 nM [3H]GABA was 40-60{\%} lower for the paraplegic groups relative to the values for the control animals. Scatchard analysis revealed significant changes in B(max) values for both amino acids. Overall, the data indicate an increase in segmental amino acid excitatory influence which occurred when signs of spasticity were evident.",
author = "McBride, {W. J.} and Hall, {P. V.} and E. Chernet and Patrick, {J. T.} and Scott Shapiro",
year = "1984",
language = "English",
volume = "42",
pages = "1625--1631",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Alterations of amino acid transmitter systems in spinal cords of chronic paraplegic dogs

AU - McBride, W. J.

AU - Hall, P. V.

AU - Chernet, E.

AU - Patrick, J. T.

AU - Shapiro, Scott

PY - 1984

Y1 - 1984

N2 - The high-affinity uptake of [3H]serotonin, [3H]glutamate, and [3H]γ-aminobutyric acid ([3H]GABA) and the Na+-independent binding of [3H]glutamate and [3H]GABA were studied using spinal cord preparations obtained from normal mongrel dogs and from dogs made paraplegia by midthoracic spinal cord crush. Lumbosacral regions of the spinal cord were removed either before (1 week) or after (3 to 8 weeks) onset of spasticity. A myelin-free synaptosomal fraction was obtained by centrifugation and used for studying high-affinity uptake and for preparing synaptic plasma membranes for Na+-independent binding experiments. For the paraplegic groups, the uptake of 30 nM [3H]serotonin was 66 and 18% of control values after 1 and 3 weeks, respectively. Eadie-Hofstee analysis of [3H]serotonin uptake showed a 90% reduction in V(max) for the paraplegic group relative to control values, thereby indicating the expected loss of descending serotonergic pathways. The high-affinity uptakes of 1 μM [3H]glutamate and [3H]GABA were the same in both the control and nonspastic paraplegic groups after 1 week. However, after 3 weeks, the uptakes of [3H]glutamate and [3H]GABA were 60-70% higher for the spastic group than for the control animals. For both amino acids, Eadie-Hofstee plots revealed no difference in K(m) and higher V(max) for the spastic group relative to control values. After 1 and 3 weeks, the Na+-independent binding of 5 nM [3H]glutamate was 40-85% higher and the binding of 10 nM [3H]GABA was 40-60% lower for the paraplegic groups relative to the values for the control animals. Scatchard analysis revealed significant changes in B(max) values for both amino acids. Overall, the data indicate an increase in segmental amino acid excitatory influence which occurred when signs of spasticity were evident.

AB - The high-affinity uptake of [3H]serotonin, [3H]glutamate, and [3H]γ-aminobutyric acid ([3H]GABA) and the Na+-independent binding of [3H]glutamate and [3H]GABA were studied using spinal cord preparations obtained from normal mongrel dogs and from dogs made paraplegia by midthoracic spinal cord crush. Lumbosacral regions of the spinal cord were removed either before (1 week) or after (3 to 8 weeks) onset of spasticity. A myelin-free synaptosomal fraction was obtained by centrifugation and used for studying high-affinity uptake and for preparing synaptic plasma membranes for Na+-independent binding experiments. For the paraplegic groups, the uptake of 30 nM [3H]serotonin was 66 and 18% of control values after 1 and 3 weeks, respectively. Eadie-Hofstee analysis of [3H]serotonin uptake showed a 90% reduction in V(max) for the paraplegic group relative to control values, thereby indicating the expected loss of descending serotonergic pathways. The high-affinity uptakes of 1 μM [3H]glutamate and [3H]GABA were the same in both the control and nonspastic paraplegic groups after 1 week. However, after 3 weeks, the uptakes of [3H]glutamate and [3H]GABA were 60-70% higher for the spastic group than for the control animals. For both amino acids, Eadie-Hofstee plots revealed no difference in K(m) and higher V(max) for the spastic group relative to control values. After 1 and 3 weeks, the Na+-independent binding of 5 nM [3H]glutamate was 40-85% higher and the binding of 10 nM [3H]GABA was 40-60% lower for the paraplegic groups relative to the values for the control animals. Scatchard analysis revealed significant changes in B(max) values for both amino acids. Overall, the data indicate an increase in segmental amino acid excitatory influence which occurred when signs of spasticity were evident.

UR - http://www.scopus.com/inward/record.url?scp=0021272185&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021272185&partnerID=8YFLogxK

M3 - Article

C2 - 6144726

AN - SCOPUS:0021272185

VL - 42

SP - 1625

EP - 1631

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 6

ER -