Alterations of the p53 tumor-suppressor gene and K-ras oncogene in perihilar cholangiocarcinomas from a high-incidence area

Patrick D J Sturm, Inge O. Baas, Marjon J. Clement, Attila Nakeeb, G. Johan A Offerhaus, Ralph H. Hruban, Henry A. Pitt

Research output: Contribution to journalArticle

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Abstract

We observed a clustering of cholangiocarcinoma in a part of West Virginia. We analyzed the frequency and type of alterations in the p53 tumor- suppressor gene and the K-ras oncogene to determine whether cholangiocarcinomas from this high-incidence area differ from other cholangiocarcinomas at the molecular level. We studied 12 carcinomas of patients from the high-incidence area (West Virginia group), and 15 carcinomas of patients from nearby states (non-West Virginia group). Over- expression of the p53 gene product, accompanying most mutations in the p53 gene, was determined by immunohistochemistry. p53 sequence analysis of exons 5, 6, 7, and 8 of the p53-immunohistochemical-positive carcinomas was also performed. K-ras codon 12 mutations were detected by the polymerase chain reaction and allele-specific oligonucleotide hybridization. Significantly more cholangiocarcinomas from the West Virginia group were p53- immunohistochemical-positive than from the non-West Virginia group (67% vs. 20%; p

Original languageEnglish (US)
Pages (from-to)695-698
Number of pages4
JournalInternational Journal of Cancer
Volume78
Issue number6
DOIs
StatePublished - 1998
Externally publishedYes

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Klatskin Tumor
ras Genes
Cholangiocarcinoma
Tumor Suppressor Genes
Incidence
p53 Genes
Carcinoma
Mutation
Codon
Oligonucleotides
Sequence Analysis
Cluster Analysis
Exons
Immunohistochemistry
Alleles
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Alterations of the p53 tumor-suppressor gene and K-ras oncogene in perihilar cholangiocarcinomas from a high-incidence area. / Sturm, Patrick D J; Baas, Inge O.; Clement, Marjon J.; Nakeeb, Attila; Offerhaus, G. Johan A; Hruban, Ralph H.; Pitt, Henry A.

In: International Journal of Cancer, Vol. 78, No. 6, 1998, p. 695-698.

Research output: Contribution to journalArticle

Sturm, Patrick D J ; Baas, Inge O. ; Clement, Marjon J. ; Nakeeb, Attila ; Offerhaus, G. Johan A ; Hruban, Ralph H. ; Pitt, Henry A. / Alterations of the p53 tumor-suppressor gene and K-ras oncogene in perihilar cholangiocarcinomas from a high-incidence area. In: International Journal of Cancer. 1998 ; Vol. 78, No. 6. pp. 695-698.
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