Alterations of yeast artificial chromosome transgenic sequences in stretched embryonic stem-cell chromatin visualized by fluorescence in situ hybridization

C. Rosenberg, A. K. Voltz, B. T. Lamb, G. Stetten, J. D. Gearhart

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Transgenic mice have been generated from embryonic stem (ES) cells carrying functional genes cloned within yeast artificial chromosomes (YACs). Information on the integrity and organization of the inserted sequences, including the, number of copies and their orientation to each other, is still limited by current methods. We have applied fluorescence in situ hybridization to stretched chromatin preparations from YAC-transfected ES cells to analyze the organization and copy number of the integrated sequences.

Original languageEnglish (US)
Pages (from-to)67-70
Number of pages4
JournalCytogenetic and Genome Research
Volume75
Issue number1
DOIs
StatePublished - Jan 1 1996

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'Alterations of yeast artificial chromosome transgenic sequences in stretched embryonic stem-cell chromatin visualized by fluorescence in situ hybridization'. Together they form a unique fingerprint.

Cite this