Alterations of yeast artificial chromosome transgenic sequences in stretched embryonic stem-cell chromatin visualized by fluorescence in situ hybridization

C. Rosenberg, A. K. Voltz, Bruce Lamb, B. T. Lamb, G. Stetten, J. D. Gearhart

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Transgenic mice have been generated from embryonic stem (ES) cells carrying functional genes cloned within yeast artificial chromosomes (YACs). Information on the integrity and organization of the inserted sequences, including the number of copies and their orientation to each other, is still limited by current methods. We have applied fluorescence in situ hybridization to stretched chromatin preparations from YAC-transfected ES cells to analyze the organization and copy number of the integrated sequences.

Original languageEnglish (US)
Pages (from-to)67-70
Number of pages4
JournalCytogenetics and Cell Genetics
Volume75
Issue number1
StatePublished - 1996
Externally publishedYes

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Yeast Artificial Chromosomes
Embryonic Stem Cells
Fluorescence In Situ Hybridization
Chromatin
Transgenic Mice
Genes

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Alterations of yeast artificial chromosome transgenic sequences in stretched embryonic stem-cell chromatin visualized by fluorescence in situ hybridization. / Rosenberg, C.; Voltz, A. K.; Lamb, Bruce; Lamb, B. T.; Stetten, G.; Gearhart, J. D.

In: Cytogenetics and Cell Genetics, Vol. 75, No. 1, 1996, p. 67-70.

Research output: Contribution to journalArticle

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AU - Stetten, G.

AU - Gearhart, J. D.

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