Alterations to the en bloc basic fuchsin staining protocol for the demonstration of microdamage produced in vivo

D. B. Burr, M. Hooser

Research output: Contribution to journalArticle

209 Scopus citations

Abstract

En bloc staining with basic fuchsin has become the method of choice to demonstrate bone microdamage produced in vivo. Several alterations have recently been made to the protocol. This technical note presents the new protocols, which include staining through a graded series of alcohols under vacuum, and eliminating the original evaporation stage in the staining protocol. Reasons for variations in staining quality can be related to inadequate dehydration, failure to stain under vacuum, the source of the basic fuchsin, or the solubility of basic fuchsin in water. The most common reasons for over- or understaining are the time left in stain, and the density of the bone. Limitations to the technique include the fact that probably not all cracks are stained by the technique, and the technique is not useful for studies that involve bone with highly variable densities in a single section.

Original languageEnglish (US)
Pages (from-to)431-433
Number of pages3
JournalBone
Volume17
Issue number4
DOIs
StatePublished - Oct 1995

Keywords

  • Bone
  • Histomorphometry
  • Microdamage

ASJC Scopus subject areas

  • Physiology
  • Endocrinology, Diabetes and Metabolism
  • Histology
  • Hematology

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