Altered IGFBP5 gene expression in the cerebellar external germinal layer of weaver mutant mice

Wei Hua Lee, Guo Ming Wang, Teresa Lo, Lazaros C. Triarhou, Bernardino Ghetti

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The IGF system components play important roles in cerebellar development as demonstrated by their specific spatial-temporal expression. IGF-I, type I IGF receptor (IGFR-I), IGFBP2 and IGFBP5 mRNA are localized in distinct cell populations, and all are expressed at the highest levels at the peak of Purkinje cell growth, active synaptogenesis and dendritic formation. To understand IGF-I's action at the cellular level, in situ hybridization was employed to investigate the distribution of IGF system gene transcripts in the cerebellum of weaver mutant mice (wv/wv). Although located ectopically, the surviving Purkinje cells express IGF-I mRNA at the same level in wv/wv as in +/+. No alteration in the cellular distribution or mRNA levels was observed with IGFBP2, or IGFR-I mRNAs. However, the pattern of IGFBP5 expression is altered in the external germinal layer of wv/wv mice. Not only is IGFBP5 expressed by more granule cell precursors of wv/wv cerebellum, but its mRNA level is 2.3 fold that of +/+. The altered IGFBP5 gene expression in granule cell precursors may modulate the interaction of IGF-I with IGFR-I in ways that contribute to their massive death occurring in the development of wv/wv cerebellum.

Original languageEnglish (US)
Pages (from-to)259-268
Number of pages10
JournalMolecular Brain Research
Volume30
Issue number2
DOIs
StatePublished - Jun 1995

Keywords

  • Cerebellar development
  • External germinal layer
  • Gene expression
  • Insulin-like growth factor I
  • Weaver mutant mouse

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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