Altered kinetics of Tap-1 gene expression in macrophages following stimulation with both IFN-γ and LPS

Lorraine A. Cramer, Michael Klemsz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

With recent studies suggesting a key role for professional antigen presenting cells in the induction of major histocompatibility class I cellular immune responses, we initiated studies on the regulation of Tap-1 and Tap-2 gene expression in macrophages. Stimulation of the human macrophage cell line THP-1 with interferon-γ (IFN-γ) resulted in maximal induction of both Tap-1 and Tap-2 mRNA within 24 hr. Nuclear run-on analyses showed that the increased expression of Tap-1 and Tap-2 was controlled at the level of transcription. Half-life studies demonstrated that mRNAs for both genes became destabilized after stimulation of THP-1 cells with IFN-γ for 24 hr, suggesting that a posttranscriptional mechanism down-regulates TAP gene expression following activation. Treatment of cells with both IFN-γ and lipopolysaccharide (LPS) altered the kinetics and amount of Tap-1 mRNA and protein expression, compared to those with stimulation with IFN-γ alone. These data suggest that LPS enhances the ability of macrophages stimulated with IFN-γ to initiate a cellular immune response by altering the kinetics of TAP gene expression.

Original languageEnglish
Pages (from-to)53-61
Number of pages9
JournalCellular Immunology
Volume178
Issue number1
DOIs
StatePublished - May 25 1997

Fingerprint

Interferons
Lipopolysaccharides
Macrophages
Gene Expression
Cellular Immunity
Messenger RNA
Professional Role
Histocompatibility
Aptitude
Antigen-Presenting Cells
Half-Life
Down-Regulation
Cell Line
Genes
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Altered kinetics of Tap-1 gene expression in macrophages following stimulation with both IFN-γ and LPS. / Cramer, Lorraine A.; Klemsz, Michael.

In: Cellular Immunology, Vol. 178, No. 1, 25.05.1997, p. 53-61.

Research output: Contribution to journalArticle

@article{cf2d0366dbe0446eaf42f8cba8bb199d,
title = "Altered kinetics of Tap-1 gene expression in macrophages following stimulation with both IFN-γ and LPS",
abstract = "With recent studies suggesting a key role for professional antigen presenting cells in the induction of major histocompatibility class I cellular immune responses, we initiated studies on the regulation of Tap-1 and Tap-2 gene expression in macrophages. Stimulation of the human macrophage cell line THP-1 with interferon-γ (IFN-γ) resulted in maximal induction of both Tap-1 and Tap-2 mRNA within 24 hr. Nuclear run-on analyses showed that the increased expression of Tap-1 and Tap-2 was controlled at the level of transcription. Half-life studies demonstrated that mRNAs for both genes became destabilized after stimulation of THP-1 cells with IFN-γ for 24 hr, suggesting that a posttranscriptional mechanism down-regulates TAP gene expression following activation. Treatment of cells with both IFN-γ and lipopolysaccharide (LPS) altered the kinetics and amount of Tap-1 mRNA and protein expression, compared to those with stimulation with IFN-γ alone. These data suggest that LPS enhances the ability of macrophages stimulated with IFN-γ to initiate a cellular immune response by altering the kinetics of TAP gene expression.",
author = "Cramer, {Lorraine A.} and Michael Klemsz",
year = "1997",
month = "5",
day = "25",
doi = "10.1006/cimm.1997.1118",
language = "English",
volume = "178",
pages = "53--61",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Altered kinetics of Tap-1 gene expression in macrophages following stimulation with both IFN-γ and LPS

AU - Cramer, Lorraine A.

AU - Klemsz, Michael

PY - 1997/5/25

Y1 - 1997/5/25

N2 - With recent studies suggesting a key role for professional antigen presenting cells in the induction of major histocompatibility class I cellular immune responses, we initiated studies on the regulation of Tap-1 and Tap-2 gene expression in macrophages. Stimulation of the human macrophage cell line THP-1 with interferon-γ (IFN-γ) resulted in maximal induction of both Tap-1 and Tap-2 mRNA within 24 hr. Nuclear run-on analyses showed that the increased expression of Tap-1 and Tap-2 was controlled at the level of transcription. Half-life studies demonstrated that mRNAs for both genes became destabilized after stimulation of THP-1 cells with IFN-γ for 24 hr, suggesting that a posttranscriptional mechanism down-regulates TAP gene expression following activation. Treatment of cells with both IFN-γ and lipopolysaccharide (LPS) altered the kinetics and amount of Tap-1 mRNA and protein expression, compared to those with stimulation with IFN-γ alone. These data suggest that LPS enhances the ability of macrophages stimulated with IFN-γ to initiate a cellular immune response by altering the kinetics of TAP gene expression.

AB - With recent studies suggesting a key role for professional antigen presenting cells in the induction of major histocompatibility class I cellular immune responses, we initiated studies on the regulation of Tap-1 and Tap-2 gene expression in macrophages. Stimulation of the human macrophage cell line THP-1 with interferon-γ (IFN-γ) resulted in maximal induction of both Tap-1 and Tap-2 mRNA within 24 hr. Nuclear run-on analyses showed that the increased expression of Tap-1 and Tap-2 was controlled at the level of transcription. Half-life studies demonstrated that mRNAs for both genes became destabilized after stimulation of THP-1 cells with IFN-γ for 24 hr, suggesting that a posttranscriptional mechanism down-regulates TAP gene expression following activation. Treatment of cells with both IFN-γ and lipopolysaccharide (LPS) altered the kinetics and amount of Tap-1 mRNA and protein expression, compared to those with stimulation with IFN-γ alone. These data suggest that LPS enhances the ability of macrophages stimulated with IFN-γ to initiate a cellular immune response by altering the kinetics of TAP gene expression.

UR - http://www.scopus.com/inward/record.url?scp=0031586043&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031586043&partnerID=8YFLogxK

U2 - 10.1006/cimm.1997.1118

DO - 10.1006/cimm.1997.1118

M3 - Article

C2 - 9184698

AN - SCOPUS:0031586043

VL - 178

SP - 53

EP - 61

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -