Altered localization and functionality of TAR DNA binding protein 43 (TDP-43) in niemann-pick disease type C

A. Dardis, S. Zampieri, S. Canterini, K. L. Newell, C. Stuani, J. R. Murrell, B. Ghetti, M. T. Fiorenza, B. Bembi, E. Buratti

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8 Scopus citations


Niemann-Pick type C (NPC) disease is a lysosomal storage disorder characterized by the occurrence of visceral and neurological symptoms. At present, the molecular mechanisms causing neurodegeneration in this disease are unknown. Here we report the altered expression and/or mislocalization of the TAR-DNA binding protein 43 (TDP-43) in both NPC mouse and in a human neuronal model of the disease. We also report the neuropathologic study of a NPC patient's brain, showing that while TDP-43 is below immunohistochemical detection in nuclei of cerebellar Purkinje cells, it has a predominant localization in the cytoplasm of these cells. From a functional point of view, the TDP-43 mislocalization, that occurs in a human experimental neuronal model system, is associated with specific alterations in TDP-43 controlled genes. Most interestingly, treatment with N-Acetyl-cysteine (NAC) or beta-cyclodextrin (CD) can partially restore TDP-43 nuclear localization. Taken together, the results of these studies extend the role of TDP-43 beyond the Amyotrophic lateral sclerosis (ALS)/frontotemporal dementia (FTD)/Alzheimer disease (AD) spectrum. These findings may open novel research/therapeutic avenues for a better understanding of both NPC disease and the TDP-43 proteinopathy disease mechanism.

Original languageEnglish (US)
Article number52
JournalActa Neuropathologica Communications
Issue number1
StatePublished - Jan 1 2016



  • Lysosomal diseases
  • NPC1
  • Niemann Pick C
  • TDP-43

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Dardis, A., Zampieri, S., Canterini, S., Newell, K. L., Stuani, C., Murrell, J. R., Ghetti, B., Fiorenza, M. T., Bembi, B., & Buratti, E. (2016). Altered localization and functionality of TAR DNA binding protein 43 (TDP-43) in niemann-pick disease type C. Acta Neuropathologica Communications, 4(1), [52].