Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1

Qingwen Jin, Bashar Alkhatib, Kenneth Cornetta, Ghalib Alkhatib

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Recent studies have demonstrated that neuropilin 1 (NP-1) is involved in HTLV-1 entry; however, the role NP-1 plays in this process is not understood. We demonstrated that ectopic expression of human NP-1 but not NP-2 cDNA increased susceptibility to HTLV-1. SiRNA-mediated inhibition of NP-1 expression correlated with significant reduction of HTLV-1 Env-mediated fusion. The vascular endothelial growth factor (VEGF165) caused downmodulation of surface NP-1 and inhibited HTLV-1 infection of U87 cells. In contrast, VEGF165 partially inhibited infection of primary astrocytes and had no significant effect on infection of HeLa cells. VEGF165 and antibodies to the glucose transporter protein 1 (anti-GLUT-1) were both needed to block infection of primary astrocytes, however, only anti-GLUT-1 antibodies were sufficient to block infection of HeLa cells. HTLV-1 Env forms complexes with both NP-1 and GLUT-1 in primary human astrocytes. The alternate usage of these two cellular receptors may have important implications regarding HTLV-1 neuro-tropism.

Original languageEnglish (US)
Pages (from-to)203-212
Number of pages10
JournalVirology
Volume396
Issue number2
DOIs
StatePublished - Jan 20 2010

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Keywords

  • HTLV-1
  • Neuropilin 1
  • Neurotropism
  • Virus entry

ASJC Scopus subject areas

  • Virology

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