Alveolar Macrophages from HIV-Infected Subjects are Resistant to Mycobacterium tuberculosis In Vitro

Richard B. Day, Yana Wang, Kenneth S. Knox, Rajamouli Pasula, William J. Martin, Homer Twigg

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

HIV-infected individuals frequently develop Mycobacterium tuberculosis (MTB) infection. Alveolar macrophages (AM) are the initial host defense against this organism. We measured MTB growth in AM from normal and HIV-infected subjects after in vitro exposure. Intracellular growth of MTB was reduced in AM from HIV-infected subjects compared with normal macrophages. This was confined to subjects with CD4 counts greater than 200/μl. Growth of avirulent mycobacteria in HIV macrophages was significantly less than virulent MTB. Because avirulent MTB is more sensitive to tumor necrosis factor-α (TNF-α), we examined the relationship between cytokine secretion and mycobacterial growth. Higher AM spontaneous TNF-α secretion was associated with reduced MTB growth in normal AM. This relationship was not seen in HIV-infected subjects, suggesting that other factors contributed to mycobacteria resistance. Mycobacteria-induced TNF-α secretion was inversely associated with growth in normal AM but not in HIV-infected subjects. Finally, binding and internalization of MTB was augmented in HIV macrophages compared with normal, demonstrating that reduced intracellular MTB growth was not due to impaired phagocytosis. In conclusion, the increased incidence of MTB infection in HIV-infected subjects does not appear to be due to a defect in macrophage innate immunity.

Original languageEnglish
Pages (from-to)403-410
Number of pages8
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume30
Issue number3
DOIs
StatePublished - Mar 2004

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Alveolar Macrophages
Mycobacterium tuberculosis
HIV
Macrophages
Growth
Mycobacterium
Tumor Necrosis Factor-alpha
Mycobacterium Infections
In Vitro Techniques
CD4 Lymphocyte Count
Phagocytosis
Innate Immunity
Cytokines
Defects
Incidence

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Alveolar Macrophages from HIV-Infected Subjects are Resistant to Mycobacterium tuberculosis In Vitro. / Day, Richard B.; Wang, Yana; Knox, Kenneth S.; Pasula, Rajamouli; Martin, William J.; Twigg, Homer.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 30, No. 3, 03.2004, p. 403-410.

Research output: Contribution to journalArticle

Day, Richard B. ; Wang, Yana ; Knox, Kenneth S. ; Pasula, Rajamouli ; Martin, William J. ; Twigg, Homer. / Alveolar Macrophages from HIV-Infected Subjects are Resistant to Mycobacterium tuberculosis In Vitro. In: American Journal of Respiratory Cell and Molecular Biology. 2004 ; Vol. 30, No. 3. pp. 403-410.
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