American Ginseng Inhibits Induced COX-2 and NFKB Activation in Breast Cancer Cells

Elizabeth A. Peralta, Laura L. Murphy, James Minnis, Somaja Louis, Gary Dunnington

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: Epidemiologic evidence suggests reduced breast cancer mortality in users of American Ginseng (AG) (Panax quinquefolium). We hypothesized that AG extract decreases proliferation of human breast cancer cells via an anti-inflammatory effect applicable to the prevention of breast and other cancers. Material and Methods: A defined lyophilized aqueous extract of AG (LEAG) was dissolved in DMSO 1mg/mL, and serially diluted in saline. The cell lines MDA MB 231 and MCF7 were stimulated with the phorbol ester PDBu and treated with 100-500 mcg/mL LEAG. Proliferation was measured by MDA assay. Induced COX-2 expression was assayed by ELISA. Activation of NFκB by phosphorylation of the p65 subunit was quantified by CASE (cellular activation of signaling ELISA). Results: Both cell lines had reduced proliferation when treated with LEAG. PDBu stimulation of MDA MB 231 increased expression of the COX-2 protein 20-fold at 48hours (P <0.005). COX-2 protein expression remained at baseline concentrations in PDBu- treated MDA MB 231 cells exposed to100 mcg/mL LEAG. The CASE assay showed a 4-fold increase in p65 activation 24hours after PDBu treatment in normal medium, while phosphorylated p65 dropped below baseline in the cells treated with PDBu plus LEAG. Conclusion: In MDA MB 231, COX-2 was inducible with PDBu. This induced COX-2 expression was blocked by 100 microgram/mL LEAG in a time course consistent with the decline in the activated p65 subunit of NFκB. These results provide an anti-inflammatory mechanism for a possible anti-cancer effect of American Ginseng.

Original languageEnglish (US)
Pages (from-to)261-267
Number of pages7
JournalJournal of Surgical Research
Volume157
Issue number2
DOIs
StatePublished - Dec 2009
Externally publishedYes

Fingerprint

Panax
Breast Neoplasms
Enzyme-Linked Immunosorbent Assay
Anti-Inflammatory Agents
Cell Line
Phorbol Esters
Dimethyl Sulfoxide
Proteins
Phosphorylation
Mortality
Neoplasms
Therapeutics

Keywords

  • breast cancer
  • COX-2
  • ginseng
  • inflammation
  • NFκB
  • prevention

ASJC Scopus subject areas

  • Surgery

Cite this

American Ginseng Inhibits Induced COX-2 and NFKB Activation in Breast Cancer Cells. / Peralta, Elizabeth A.; Murphy, Laura L.; Minnis, James; Louis, Somaja; Dunnington, Gary.

In: Journal of Surgical Research, Vol. 157, No. 2, 12.2009, p. 261-267.

Research output: Contribution to journalArticle

Peralta, Elizabeth A. ; Murphy, Laura L. ; Minnis, James ; Louis, Somaja ; Dunnington, Gary. / American Ginseng Inhibits Induced COX-2 and NFKB Activation in Breast Cancer Cells. In: Journal of Surgical Research. 2009 ; Vol. 157, No. 2. pp. 261-267.
@article{0b19cf8b96c54d4f8e4c84b6205340c4,
title = "American Ginseng Inhibits Induced COX-2 and NFKB Activation in Breast Cancer Cells",
abstract = "Background: Epidemiologic evidence suggests reduced breast cancer mortality in users of American Ginseng (AG) (Panax quinquefolium). We hypothesized that AG extract decreases proliferation of human breast cancer cells via an anti-inflammatory effect applicable to the prevention of breast and other cancers. Material and Methods: A defined lyophilized aqueous extract of AG (LEAG) was dissolved in DMSO 1mg/mL, and serially diluted in saline. The cell lines MDA MB 231 and MCF7 were stimulated with the phorbol ester PDBu and treated with 100-500 mcg/mL LEAG. Proliferation was measured by MDA assay. Induced COX-2 expression was assayed by ELISA. Activation of NFκB by phosphorylation of the p65 subunit was quantified by CASE (cellular activation of signaling ELISA). Results: Both cell lines had reduced proliferation when treated with LEAG. PDBu stimulation of MDA MB 231 increased expression of the COX-2 protein 20-fold at 48hours (P <0.005). COX-2 protein expression remained at baseline concentrations in PDBu- treated MDA MB 231 cells exposed to100 mcg/mL LEAG. The CASE assay showed a 4-fold increase in p65 activation 24hours after PDBu treatment in normal medium, while phosphorylated p65 dropped below baseline in the cells treated with PDBu plus LEAG. Conclusion: In MDA MB 231, COX-2 was inducible with PDBu. This induced COX-2 expression was blocked by 100 microgram/mL LEAG in a time course consistent with the decline in the activated p65 subunit of NFκB. These results provide an anti-inflammatory mechanism for a possible anti-cancer effect of American Ginseng.",
keywords = "breast cancer, COX-2, ginseng, inflammation, NFκB, prevention",
author = "Peralta, {Elizabeth A.} and Murphy, {Laura L.} and James Minnis and Somaja Louis and Gary Dunnington",
year = "2009",
month = "12",
doi = "10.1016/j.jss.2009.05.011",
language = "English (US)",
volume = "157",
pages = "261--267",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - American Ginseng Inhibits Induced COX-2 and NFKB Activation in Breast Cancer Cells

AU - Peralta, Elizabeth A.

AU - Murphy, Laura L.

AU - Minnis, James

AU - Louis, Somaja

AU - Dunnington, Gary

PY - 2009/12

Y1 - 2009/12

N2 - Background: Epidemiologic evidence suggests reduced breast cancer mortality in users of American Ginseng (AG) (Panax quinquefolium). We hypothesized that AG extract decreases proliferation of human breast cancer cells via an anti-inflammatory effect applicable to the prevention of breast and other cancers. Material and Methods: A defined lyophilized aqueous extract of AG (LEAG) was dissolved in DMSO 1mg/mL, and serially diluted in saline. The cell lines MDA MB 231 and MCF7 were stimulated with the phorbol ester PDBu and treated with 100-500 mcg/mL LEAG. Proliferation was measured by MDA assay. Induced COX-2 expression was assayed by ELISA. Activation of NFκB by phosphorylation of the p65 subunit was quantified by CASE (cellular activation of signaling ELISA). Results: Both cell lines had reduced proliferation when treated with LEAG. PDBu stimulation of MDA MB 231 increased expression of the COX-2 protein 20-fold at 48hours (P <0.005). COX-2 protein expression remained at baseline concentrations in PDBu- treated MDA MB 231 cells exposed to100 mcg/mL LEAG. The CASE assay showed a 4-fold increase in p65 activation 24hours after PDBu treatment in normal medium, while phosphorylated p65 dropped below baseline in the cells treated with PDBu plus LEAG. Conclusion: In MDA MB 231, COX-2 was inducible with PDBu. This induced COX-2 expression was blocked by 100 microgram/mL LEAG in a time course consistent with the decline in the activated p65 subunit of NFκB. These results provide an anti-inflammatory mechanism for a possible anti-cancer effect of American Ginseng.

AB - Background: Epidemiologic evidence suggests reduced breast cancer mortality in users of American Ginseng (AG) (Panax quinquefolium). We hypothesized that AG extract decreases proliferation of human breast cancer cells via an anti-inflammatory effect applicable to the prevention of breast and other cancers. Material and Methods: A defined lyophilized aqueous extract of AG (LEAG) was dissolved in DMSO 1mg/mL, and serially diluted in saline. The cell lines MDA MB 231 and MCF7 were stimulated with the phorbol ester PDBu and treated with 100-500 mcg/mL LEAG. Proliferation was measured by MDA assay. Induced COX-2 expression was assayed by ELISA. Activation of NFκB by phosphorylation of the p65 subunit was quantified by CASE (cellular activation of signaling ELISA). Results: Both cell lines had reduced proliferation when treated with LEAG. PDBu stimulation of MDA MB 231 increased expression of the COX-2 protein 20-fold at 48hours (P <0.005). COX-2 protein expression remained at baseline concentrations in PDBu- treated MDA MB 231 cells exposed to100 mcg/mL LEAG. The CASE assay showed a 4-fold increase in p65 activation 24hours after PDBu treatment in normal medium, while phosphorylated p65 dropped below baseline in the cells treated with PDBu plus LEAG. Conclusion: In MDA MB 231, COX-2 was inducible with PDBu. This induced COX-2 expression was blocked by 100 microgram/mL LEAG in a time course consistent with the decline in the activated p65 subunit of NFκB. These results provide an anti-inflammatory mechanism for a possible anti-cancer effect of American Ginseng.

KW - breast cancer

KW - COX-2

KW - ginseng

KW - inflammation

KW - NFκB

KW - prevention

UR - http://www.scopus.com/inward/record.url?scp=70450228666&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70450228666&partnerID=8YFLogxK

U2 - 10.1016/j.jss.2009.05.011

DO - 10.1016/j.jss.2009.05.011

M3 - Article

C2 - 19815237

AN - SCOPUS:70450228666

VL - 157

SP - 261

EP - 267

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 2

ER -