Ames hypopituitary dwarf mice demonstrate imbalanced myelopoiesis between bone marrow and spleen

Maegan L. Capitano, Brahmananda R. Chitteti, Scott Cooper, Edward Srour, Andrzej Bartke, Hal Broxmeyer

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ames hypopituitary dwarf mice are deficient in growth hormone, thyroid-stimulating hormone, and prolactin. The phenotype of these mice demonstrates irregularities in the immune system with skewing of the normal cytokine milieu towards a more anti-inflammatory environment. However, the hematopoietic stem and progenitor cell composition of the bone marrow (BM) and spleen in Ames dwarf mice has not been well characterized. We found that there was a significant decrease in overall cell count when comparing the BM and spleen of 4-5. month old dwarf mice to their littermate controls. Upon adjusting counts to differences in body weight between the dwarf and control mice, the number of granulocyte-macrophage progenitors, confirmed by immunophenotyping and colony-formation assay was increased in the BM. In contrast, the numbers of all myeloid progenitor populations in the spleen were greatly reduced, as confirmed by colony-formation assays. This suggests that there is a shift of myelopoiesis from the spleen to the BM of Ames dwarf mice; however, this shift does not appear to involve erythropoiesis. The reasons for this unusual shift in spleen to marrow hematopoiesis in Ames dwarf mice are yet to be determined but may relate to the decreased hormone levels in these mice.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalBlood Cells, Molecules and Diseases
Volume55
Issue number1
DOIs
StatePublished - Jun 1 2015

Fingerprint

Myelopoiesis
Spleen
Bone Marrow
Hematopoietic Stem Cells
Immunophenotyping
Granulocyte-Macrophage Progenitor Cells
Erythropoiesis
Hematopoiesis
Thyrotropin
Prolactin
Growth Hormone
Immune System
Anti-Inflammatory Agents
Cell Count
Body Weight
Hormones
Cytokines
Phenotype

Keywords

  • Ames dwarf mice
  • Growth hormone
  • Hematopoietic progenitor cell
  • Prolactin
  • Thyroid-stimulating hormone

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Hematology
  • Cell Biology

Cite this

Ames hypopituitary dwarf mice demonstrate imbalanced myelopoiesis between bone marrow and spleen. / Capitano, Maegan L.; Chitteti, Brahmananda R.; Cooper, Scott; Srour, Edward; Bartke, Andrzej; Broxmeyer, Hal.

In: Blood Cells, Molecules and Diseases, Vol. 55, No. 1, 01.06.2015, p. 15-20.

Research output: Contribution to journalArticle

Capitano, Maegan L. ; Chitteti, Brahmananda R. ; Cooper, Scott ; Srour, Edward ; Bartke, Andrzej ; Broxmeyer, Hal. / Ames hypopituitary dwarf mice demonstrate imbalanced myelopoiesis between bone marrow and spleen. In: Blood Cells, Molecules and Diseases. 2015 ; Vol. 55, No. 1. pp. 15-20.
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