AMF-1/Gps2 binds p300 and enhances its interaction with papillomavirus E2 proteins

Yu Cai Peng, David E. Breiding, Francis Sverdrup, James Richard, Elliot Androphy

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The cellular protein AMF-1 (Gps2) positively modulates gene expression by the papillomavirus E2 protein (D. E. Breiding et al., Mol. Cell. Biol. 17:7208-7219, 1997). We show here that AMF-1 also binds the transcriptional coactivator p300 in vitro and in vivo. E2 interacted weakly with p300. These observations led to a model in which AMF-1 recruits p300 into a complex with E2. Cotransfection of AMF-1 or p300 stimulated levels of E2-dependent transcription, while cotransfection of both AMF-1 and p300 showed an additive effect. The functional significance of p300 recruitment for E2 transactivation was evidenced by repression of E2-activated transcription by adenovirus E1A, which inhibits both coactivator and acetylase activities of p300. Antibodies to AMF-1 or E2 immunoprecipitated histone acetylase activity from cell lysates. Western blotting using antibody against acetyl-lysine failed to detect acetylation of AMF-1 or E2 in complex with p300. These results suggest that AMF-1 facilitates the recruitment of p300 and its histone acetylase activity into complexes with E2 and represents a novel mechanism of transcriptional activation.

Original languageEnglish (US)
Pages (from-to)5872-5879
Number of pages8
JournalJournal of Virology
Volume74
Issue number13
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Papillomaviridae
transcriptional activation
Transcriptional Activation
transcription (genetics)
Acetylesterase
Histone Acetyltransferases
antibodies
Antibodies
acetylation
Adenoviridae
Acetylation
additive effect
Lysine
lysine
Western blotting
Proteins
proteins
Western Blotting
cells
Gene Expression

ASJC Scopus subject areas

  • Immunology

Cite this

AMF-1/Gps2 binds p300 and enhances its interaction with papillomavirus E2 proteins. / Peng, Yu Cai; Breiding, David E.; Sverdrup, Francis; Richard, James; Androphy, Elliot.

In: Journal of Virology, Vol. 74, No. 13, 2000, p. 5872-5879.

Research output: Contribution to journalArticle

Peng, Yu Cai ; Breiding, David E. ; Sverdrup, Francis ; Richard, James ; Androphy, Elliot. / AMF-1/Gps2 binds p300 and enhances its interaction with papillomavirus E2 proteins. In: Journal of Virology. 2000 ; Vol. 74, No. 13. pp. 5872-5879.
@article{e221cd6cb12e4ea3a592e0ce8021f095,
title = "AMF-1/Gps2 binds p300 and enhances its interaction with papillomavirus E2 proteins",
abstract = "The cellular protein AMF-1 (Gps2) positively modulates gene expression by the papillomavirus E2 protein (D. E. Breiding et al., Mol. Cell. Biol. 17:7208-7219, 1997). We show here that AMF-1 also binds the transcriptional coactivator p300 in vitro and in vivo. E2 interacted weakly with p300. These observations led to a model in which AMF-1 recruits p300 into a complex with E2. Cotransfection of AMF-1 or p300 stimulated levels of E2-dependent transcription, while cotransfection of both AMF-1 and p300 showed an additive effect. The functional significance of p300 recruitment for E2 transactivation was evidenced by repression of E2-activated transcription by adenovirus E1A, which inhibits both coactivator and acetylase activities of p300. Antibodies to AMF-1 or E2 immunoprecipitated histone acetylase activity from cell lysates. Western blotting using antibody against acetyl-lysine failed to detect acetylation of AMF-1 or E2 in complex with p300. These results suggest that AMF-1 facilitates the recruitment of p300 and its histone acetylase activity into complexes with E2 and represents a novel mechanism of transcriptional activation.",
author = "Peng, {Yu Cai} and Breiding, {David E.} and Francis Sverdrup and James Richard and Elliot Androphy",
year = "2000",
doi = "10.1128/JVI.74.13.5872-5879.2000",
language = "English (US)",
volume = "74",
pages = "5872--5879",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "13",

}

TY - JOUR

T1 - AMF-1/Gps2 binds p300 and enhances its interaction with papillomavirus E2 proteins

AU - Peng, Yu Cai

AU - Breiding, David E.

AU - Sverdrup, Francis

AU - Richard, James

AU - Androphy, Elliot

PY - 2000

Y1 - 2000

N2 - The cellular protein AMF-1 (Gps2) positively modulates gene expression by the papillomavirus E2 protein (D. E. Breiding et al., Mol. Cell. Biol. 17:7208-7219, 1997). We show here that AMF-1 also binds the transcriptional coactivator p300 in vitro and in vivo. E2 interacted weakly with p300. These observations led to a model in which AMF-1 recruits p300 into a complex with E2. Cotransfection of AMF-1 or p300 stimulated levels of E2-dependent transcription, while cotransfection of both AMF-1 and p300 showed an additive effect. The functional significance of p300 recruitment for E2 transactivation was evidenced by repression of E2-activated transcription by adenovirus E1A, which inhibits both coactivator and acetylase activities of p300. Antibodies to AMF-1 or E2 immunoprecipitated histone acetylase activity from cell lysates. Western blotting using antibody against acetyl-lysine failed to detect acetylation of AMF-1 or E2 in complex with p300. These results suggest that AMF-1 facilitates the recruitment of p300 and its histone acetylase activity into complexes with E2 and represents a novel mechanism of transcriptional activation.

AB - The cellular protein AMF-1 (Gps2) positively modulates gene expression by the papillomavirus E2 protein (D. E. Breiding et al., Mol. Cell. Biol. 17:7208-7219, 1997). We show here that AMF-1 also binds the transcriptional coactivator p300 in vitro and in vivo. E2 interacted weakly with p300. These observations led to a model in which AMF-1 recruits p300 into a complex with E2. Cotransfection of AMF-1 or p300 stimulated levels of E2-dependent transcription, while cotransfection of both AMF-1 and p300 showed an additive effect. The functional significance of p300 recruitment for E2 transactivation was evidenced by repression of E2-activated transcription by adenovirus E1A, which inhibits both coactivator and acetylase activities of p300. Antibodies to AMF-1 or E2 immunoprecipitated histone acetylase activity from cell lysates. Western blotting using antibody against acetyl-lysine failed to detect acetylation of AMF-1 or E2 in complex with p300. These results suggest that AMF-1 facilitates the recruitment of p300 and its histone acetylase activity into complexes with E2 and represents a novel mechanism of transcriptional activation.

UR - http://www.scopus.com/inward/record.url?scp=0034051288&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034051288&partnerID=8YFLogxK

U2 - 10.1128/JVI.74.13.5872-5879.2000

DO - 10.1128/JVI.74.13.5872-5879.2000

M3 - Article

VL - 74

SP - 5872

EP - 5879

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 13

ER -