AMF1 (GPS2) modulates p53 transactivation

Y. C. Peng, F. Kuo, D. E. Breiding, Y. F. Wang, C. P. Mansur, Elliot Androphy

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

We have reported that the papillomavirus E2 protein binds the nuclear factor AMF1 (also called G-protein pathway suppressor 2 or GPS2) and that their interaction is necessary for transcriptional activation by E2. It has also been shown that AMF1 can influence the activity of cellular transcription factors. These observations led us to test whether AMF1 regulates the functions of p53, a critical transcriptional activator that integrates stress signals and regulates cell cycle and programmed cell death. We report that AMF1 associates with p53 in vivo and in vitro and facilitates the p53 response by augmenting p53-dependent transcription. Overexpression of AMF1 in U2OS cells increases basal level p21WAF1/CIP1 expression and causes a G1 arrest. U2OS cells stably overexpressing AMF1 show increased apoptosis upon exposure to UV irradiation. These data demonstrate that AMF1 modulates p53 activities.

Original languageEnglish (US)
Pages (from-to)5913-5924
Number of pages12
JournalMolecular and Cellular Biology
Volume21
Issue number17
DOIs
StatePublished - 2001
Externally publishedYes

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Transcriptional Activation
Nuclear Proteins
GTP-Binding Proteins
Cell Cycle
Cell Death
Transcription Factors
Apoptosis
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Peng, Y. C., Kuo, F., Breiding, D. E., Wang, Y. F., Mansur, C. P., & Androphy, E. (2001). AMF1 (GPS2) modulates p53 transactivation. Molecular and Cellular Biology, 21(17), 5913-5924. https://doi.org/10.1128/MCB.21.17.5913-5924.2001

AMF1 (GPS2) modulates p53 transactivation. / Peng, Y. C.; Kuo, F.; Breiding, D. E.; Wang, Y. F.; Mansur, C. P.; Androphy, Elliot.

In: Molecular and Cellular Biology, Vol. 21, No. 17, 2001, p. 5913-5924.

Research output: Contribution to journalArticle

Peng, YC, Kuo, F, Breiding, DE, Wang, YF, Mansur, CP & Androphy, E 2001, 'AMF1 (GPS2) modulates p53 transactivation', Molecular and Cellular Biology, vol. 21, no. 17, pp. 5913-5924. https://doi.org/10.1128/MCB.21.17.5913-5924.2001
Peng, Y. C. ; Kuo, F. ; Breiding, D. E. ; Wang, Y. F. ; Mansur, C. P. ; Androphy, Elliot. / AMF1 (GPS2) modulates p53 transactivation. In: Molecular and Cellular Biology. 2001 ; Vol. 21, No. 17. pp. 5913-5924.
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