AMF1 (GPS2) modulates p53 transactivation

Y. C. Peng, F. Kuo, D. E. Breiding, Y. F. Wang, C. P. Mansur, E. J. Androphy

Research output: Contribution to journalArticle

41 Scopus citations


We have reported that the papillomavirus E2 protein binds the nuclear factor AMF1 (also called G-protein pathway suppressor 2 or GPS2) and that their interaction is necessary for transcriptional activation by E2. It has also been shown that AMF1 can influence the activity of cellular transcription factors. These observations led us to test whether AMF1 regulates the functions of p53, a critical transcriptional activator that integrates stress signals and regulates cell cycle and programmed cell death. We report that AMF1 associates with p53 in vivo and in vitro and facilitates the p53 response by augmenting p53-dependent transcription. Overexpression of AMF1 in U2OS cells increases basal level p21WAF1/CIP1 expression and causes a G1 arrest. U2OS cells stably overexpressing AMF1 show increased apoptosis upon exposure to UV irradiation. These data demonstrate that AMF1 modulates p53 activities.

Original languageEnglish (US)
Pages (from-to)5913-5924
Number of pages12
JournalMolecular and cellular biology
Issue number17
StatePublished - 2001

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'AMF1 (GPS2) modulates p53 transactivation'. Together they form a unique fingerprint.

Cite this