Amino-terminal identity of co-existent amyloid and non-amyloid immunoglobulin K light chain deposits. A human disease to study alterations of protein conformation

B. Kaplan, Ruben Vidal, A. Kumar, J. Ghiso, B. Frangione, Gloria Gallo

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31 Citations (Scopus)

Abstract

Tissue deposition of monoclonal immunoglobulin light chains is a serious complication in some patients with B cell proliferative disorders. The deposits are typically fibrillar and Congophilic in amyloid (AL) and non- fibrillar and Congophobic in light chain deposition disease (LCDD), and rarely coexist in the same patient. From post-mortem tissue of an individual with fibrillar and non-fibrillar κ light chain deposits in different sites, we separately extracted and analysed biochemically and immunochemically the non-amyloid deposits from isolated glomeruli, the amyloid from isolated renal arteries and the amyloid from myocardium in which the only deposits were amyloid restricted to mural arteries. Western blotting analysis of both the extracted amyloid and the non-amyloid deposits demonstrated 25-kD bands immunoreactive with anti-κ antibody, and the identity of the N-terminal amino acid sequences that belong to the variable region κ(IV) light chain subgroup. This is the first human disease in which antigenically similar but morphologically different deposits have been separately biochemically analysed. We propose that combined LCDD and AL is an ideal human disease to study the relationships and the factors that influence the conversion of non- amyloidogenic to amyloidogenic conformations.

Original languageEnglish (US)
Pages (from-to)472-478
Number of pages7
JournalClinical and Experimental Immunology
Volume110
Issue number3
StatePublished - 1997
Externally publishedYes

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Immunoglobulin Light Chains
Protein Conformation
Amyloid
Light
Amyloid Plaques
Renal Artery
Anti-Idiotypic Antibodies
Amino Acid Sequence
Myocardium
B-Lymphocytes
Arteries
Western Blotting

Keywords

  • Gammopathy
  • Monoclonal immunoglobulins
  • Protein conformation

ASJC Scopus subject areas

  • Immunology

Cite this

Amino-terminal identity of co-existent amyloid and non-amyloid immunoglobulin K light chain deposits. A human disease to study alterations of protein conformation. / Kaplan, B.; Vidal, Ruben; Kumar, A.; Ghiso, J.; Frangione, B.; Gallo, Gloria.

In: Clinical and Experimental Immunology, Vol. 110, No. 3, 1997, p. 472-478.

Research output: Contribution to journalArticle

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AU - Vidal, Ruben

AU - Kumar, A.

AU - Ghiso, J.

AU - Frangione, B.

AU - Gallo, Gloria

PY - 1997

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